Morphology and molecular characterization of pseudo-nitzschia (bacillariophyceae) from malaysian borneo, including the new species pseudo-nitzschia circumpora sp. nov

Hong Chang Lim, Chui Pin Leaw, Suriyanti Nyun Pau Su, Sing Tung Teng, Gires Usup, Normawaty Mohammad-Noor, Nina Lundholm, Yuichi Kotaki, Po Teen Lim

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Field sampling was undertaken to investigate the occurrence of Pseudo-nitzschia Peragallo species in eight locations along the coast of Malaysian Borneo. A total of 108 strains of Pseudo-nitzschia species were isolated, and their morphology examined with SEM and TEM. Additionally, molecular data from nuclear-encoded partial LSU rDNA, and ITS regions, were characterized. A total of five species were confidently identified based on a combination of distinct morphological characteristics and supporting molecular evidence: P. brasiliana Lundholm, Hasle & Fryxell, P. cuspidata (Hasle) Hasle, P. dolorosa Lundholm & Moestrup, P. micropora Priisholm, Moestrup & Lundholm, and P. pungens (Grunow) Hasle var. pungens. However, one morphotype from Sarawak, while somewhat similar to P. caciantha, showed significant morphological distinction from this and any other of the currently described species. Most notably this morphotype possessed a characteristic pore arrangement in the poroids, with the fine pores in each perforation sector arranged in circles. Pair-wise sequence comparison of the LSU rDNA between this unidentified morphotype and P. caciantha Lundholm, Moestrup & Hasle, revealed 2.7% genetic divergence. Phylogenetic analyses strongly supported the monophyly of the morphotype. Based upon these supporting data it is here described as a new species, Pseudo-nitzschia circumpora sp. nov. A key to the six species of Pseudo-nitzschia from Malaysian Borneo is presented. Molecular signatures for all species were established based on structural comparisons of ITS2 rRNA transcripts.

Original languageEnglish
Pages (from-to)1232-1247
Number of pages16
JournalJournal of Phycology
Volume48
Issue number5
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Pseudonitzschia
Bacillariophyceae
Borneo
morphotype
new species
monophyly
perforation
ribosomal RNA
coasts
genetic variation
transmission electron microscopy
phylogeny
divergence
scanning electron microscopy
phylogenetics
coast
sampling

Keywords

  • Malaysia
  • Molecular signature
  • Morphology
  • Pseudo-nitzschia
  • Pseudo-nitzschia circumpora

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

Morphology and molecular characterization of pseudo-nitzschia (bacillariophyceae) from malaysian borneo, including the new species pseudo-nitzschia circumpora sp. nov. / Lim, Hong Chang; Leaw, Chui Pin; Su, Suriyanti Nyun Pau; Teng, Sing Tung; Usup, Gires; Mohammad-Noor, Normawaty; Lundholm, Nina; Kotaki, Yuichi; Lim, Po Teen.

In: Journal of Phycology, Vol. 48, No. 5, 10.2012, p. 1232-1247.

Research output: Contribution to journalArticle

Lim, Hong Chang ; Leaw, Chui Pin ; Su, Suriyanti Nyun Pau ; Teng, Sing Tung ; Usup, Gires ; Mohammad-Noor, Normawaty ; Lundholm, Nina ; Kotaki, Yuichi ; Lim, Po Teen. / Morphology and molecular characterization of pseudo-nitzschia (bacillariophyceae) from malaysian borneo, including the new species pseudo-nitzschia circumpora sp. nov. In: Journal of Phycology. 2012 ; Vol. 48, No. 5. pp. 1232-1247.
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abstract = "Field sampling was undertaken to investigate the occurrence of Pseudo-nitzschia Peragallo species in eight locations along the coast of Malaysian Borneo. A total of 108 strains of Pseudo-nitzschia species were isolated, and their morphology examined with SEM and TEM. Additionally, molecular data from nuclear-encoded partial LSU rDNA, and ITS regions, were characterized. A total of five species were confidently identified based on a combination of distinct morphological characteristics and supporting molecular evidence: P. brasiliana Lundholm, Hasle & Fryxell, P. cuspidata (Hasle) Hasle, P. dolorosa Lundholm & Moestrup, P. micropora Priisholm, Moestrup & Lundholm, and P. pungens (Grunow) Hasle var. pungens. However, one morphotype from Sarawak, while somewhat similar to P. caciantha, showed significant morphological distinction from this and any other of the currently described species. Most notably this morphotype possessed a characteristic pore arrangement in the poroids, with the fine pores in each perforation sector arranged in circles. Pair-wise sequence comparison of the LSU rDNA between this unidentified morphotype and P. caciantha Lundholm, Moestrup & Hasle, revealed 2.7{\%} genetic divergence. Phylogenetic analyses strongly supported the monophyly of the morphotype. Based upon these supporting data it is here described as a new species, Pseudo-nitzschia circumpora sp. nov. A key to the six species of Pseudo-nitzschia from Malaysian Borneo is presented. Molecular signatures for all species were established based on structural comparisons of ITS2 rRNA transcripts.",
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AU - Su, Suriyanti Nyun Pau

AU - Teng, Sing Tung

AU - Usup, Gires

AU - Mohammad-Noor, Normawaty

AU - Lundholm, Nina

AU - Kotaki, Yuichi

AU - Lim, Po Teen

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