Morphology and histology of the defensive glands of stick insect, abrosoma johorensis

Ayob Zeti Aktar, Nurul Wahida Othman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study was conducted to identify the morphology and histology of defensive glands of stick insect, Abrosoma johorensis (Phasmida: Aschiphasmatidae). Samples were collected from Mount of Ledang, Johor. Observation of gross morphology in-situ and ex-situ on the defensive gland of A. johorensis have been done using stereo microscope Carl Zeiss V12) connected to Canon EOS 1000D with LAS EZ software. Cell of A. johorensis’s defensive gland were stained using a special staining procedure that is Periodic Acid Schiff’s reagent and Alcian Blue method. Histological slides were viewed using light photomicroscope Carl Zeiss Axio Scope A1 with iSolutionLite v1.0 software. The defensive gland morphology showed that male individual of A. johorensis have a smaller size of gland compared to female individual. Histological study showed that the defensive gland structures consist of secretory epithelium and surrounded by layer of muscles known as longitudinal muscles and circular muscles. Observation on ultrastructure of the defensive gland was able to discover several components and organelle lies within the secretory epithelium region that might take part in the production of chemical secretion such as epithelial cells, pigment granules, ductules, vesicles, mitochondria, tracheols and secretory apparatus.

Original languageEnglish
Pages (from-to)99-109
Number of pages11
JournalSerangga
Volume23
Issue number2 Special Issue
Publication statusPublished - 1 Jan 2018

Fingerprint

Phasmida
histology
muscle
insect
muscles
epithelium
software
EOS
mitochondrion
ultrastructure
vesicle
secretion
microscopes
organelles
pigment
granules
epithelial cells
mitochondria
pigments
acids

Keywords

  • Defensive gland
  • Histology
  • Morphology
  • Stick insects
  • Ultrastructure

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

Cite this

Morphology and histology of the defensive glands of stick insect, abrosoma johorensis. / Zeti Aktar, Ayob; Othman, Nurul Wahida.

In: Serangga, Vol. 23, No. 2 Special Issue, 01.01.2018, p. 99-109.

Research output: Contribution to journalArticle

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