Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, rhynchophorus ferrugineus (Coleoptera: Dryophthoridae)

Research output: Contribution to journalArticle

Abstract

This study was conducted to assess intracellular bacteria Wolbachia in the ovaries of female Red Palm Weevil (RPW), Rhynchophorus ferrugineus from three populations in Malaysia by means of PCR using wsp-specific primer. The morphology and histology of ovary of RPW were also studied and examined by stereo microscope LEICA EZ4 HD that was equipped with LAS EZ software and light microscope Zeiss Axio Scope with iSolutionLite software respectively. We found that the adult female had two pairs of ovaries, lateral oviduct, common oviduct, bursa copulatrix, spermatheca and vagina. Histological study of the ovariole revealed that it is categorised under polytrophic ovariole. The ovariole is divided into four regions, the terminal filament, the germarium, the vitellarium and the stalk or calyx. Besides, the infection status showed that all three populations were not infected with Wolbachia. Our result suggests that no infection of Wolbachia in RPW reproductive system.

Original languageEnglish
Pages (from-to)183-193
Number of pages11
JournalSerangga
Volume23
Issue number2 Special Issue
Publication statusPublished - 1 Jan 2018

Fingerprint

Rhynchophorus ferrugineus
Wolbachia
histology
ovarioles
gonads
Coleoptera
screening
software
oviducts
Vitellaria
spermatheca
bacterium
vagina
reproductive system
light microscopes
calyx
infection
microscopes
Malaysia
organ

Keywords

  • Biopesticide
  • Ovary
  • Rhynchophorus ferrugineus
  • Wolbachia

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

Cite this

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title = "Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, rhynchophorus ferrugineus (Coleoptera: Dryophthoridae)",
abstract = "This study was conducted to assess intracellular bacteria Wolbachia in the ovaries of female Red Palm Weevil (RPW), Rhynchophorus ferrugineus from three populations in Malaysia by means of PCR using wsp-specific primer. The morphology and histology of ovary of RPW were also studied and examined by stereo microscope LEICA EZ4 HD that was equipped with LAS EZ software and light microscope Zeiss Axio Scope with iSolutionLite software respectively. We found that the adult female had two pairs of ovaries, lateral oviduct, common oviduct, bursa copulatrix, spermatheca and vagina. Histological study of the ovariole revealed that it is categorised under polytrophic ovariole. The ovariole is divided into four regions, the terminal filament, the germarium, the vitellarium and the stalk or calyx. Besides, the infection status showed that all three populations were not infected with Wolbachia. Our result suggests that no infection of Wolbachia in RPW reproductive system.",
keywords = "Biopesticide, Ovary, Rhynchophorus ferrugineus, Wolbachia",
author = "{Wan Nurul ‘Ain}, {Wan Mohd Nor} and Othman, {Nurul Wahida} and Salmah Yaakop and {Md Nor}, {Norefrina Shafinaz}",
year = "2018",
month = "1",
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volume = "23",
pages = "183--193",
journal = "Serangga",
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AU - Wan Nurul ‘Ain, Wan Mohd Nor

AU - Othman, Nurul Wahida

AU - Yaakop, Salmah

AU - Md Nor, Norefrina Shafinaz

PY - 2018/1/1

Y1 - 2018/1/1

N2 - This study was conducted to assess intracellular bacteria Wolbachia in the ovaries of female Red Palm Weevil (RPW), Rhynchophorus ferrugineus from three populations in Malaysia by means of PCR using wsp-specific primer. The morphology and histology of ovary of RPW were also studied and examined by stereo microscope LEICA EZ4 HD that was equipped with LAS EZ software and light microscope Zeiss Axio Scope with iSolutionLite software respectively. We found that the adult female had two pairs of ovaries, lateral oviduct, common oviduct, bursa copulatrix, spermatheca and vagina. Histological study of the ovariole revealed that it is categorised under polytrophic ovariole. The ovariole is divided into four regions, the terminal filament, the germarium, the vitellarium and the stalk or calyx. Besides, the infection status showed that all three populations were not infected with Wolbachia. Our result suggests that no infection of Wolbachia in RPW reproductive system.

AB - This study was conducted to assess intracellular bacteria Wolbachia in the ovaries of female Red Palm Weevil (RPW), Rhynchophorus ferrugineus from three populations in Malaysia by means of PCR using wsp-specific primer. The morphology and histology of ovary of RPW were also studied and examined by stereo microscope LEICA EZ4 HD that was equipped with LAS EZ software and light microscope Zeiss Axio Scope with iSolutionLite software respectively. We found that the adult female had two pairs of ovaries, lateral oviduct, common oviduct, bursa copulatrix, spermatheca and vagina. Histological study of the ovariole revealed that it is categorised under polytrophic ovariole. The ovariole is divided into four regions, the terminal filament, the germarium, the vitellarium and the stalk or calyx. Besides, the infection status showed that all three populations were not infected with Wolbachia. Our result suggests that no infection of Wolbachia in RPW reproductive system.

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