Morphological and histological changes during the somatic embryogenesis of mangosteen

M. Elviana, E. R. Rohani, Ismanizan Ismail, Normah Mohd. Noor

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Induction of somatic embryogenesis in leaf explants from young mangosteen seedlings using different concentrations and combinations of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) was investigated. The best medium inducing the formation of globular structures (40 %) was Murashige and Skoog medium with 0. 7 mg dm -3 BAP and 0. 7 mg dm -3 TDZ. For their further development, subculturing onto different maturation media was carried out, but these globular structures did not develop futher stages of somatic embryogenesis. However, they developed shoots after 90 d of culture on the original medium. Morphological and histological analyses were performed, and showed that the globular structures resembled closely the undifferentiated structure of the mangosteen seed. We propose that the development of mangosteen somatic embryos does not follow the typical course of somatic embryogenesis, but the course of development that is natural for mangosteen seed, where procambium is the only structure observed and there is no differentiated embryo.

Original languageEnglish
Pages (from-to)731-736
Number of pages6
JournalBiologia Plantarum
Volume55
Issue number4
DOIs
Publication statusPublished - Dec 2011

Fingerprint

mangosteens
somatic embryogenesis
seeds
somatic embryos
embryo (plant)
benzyladenine
explants
shoots
seedlings
leaves
thidiazuron

Keywords

  • Garcinia mangostana
  • globular embryo
  • seed structure
  • undifferentiated structure

ASJC Scopus subject areas

  • Plant Science
  • Horticulture

Cite this

Morphological and histological changes during the somatic embryogenesis of mangosteen. / Elviana, M.; Rohani, E. R.; Ismail, Ismanizan; Mohd. Noor, Normah.

In: Biologia Plantarum, Vol. 55, No. 4, 12.2011, p. 731-736.

Research output: Contribution to journalArticle

Elviana, M. ; Rohani, E. R. ; Ismail, Ismanizan ; Mohd. Noor, Normah. / Morphological and histological changes during the somatic embryogenesis of mangosteen. In: Biologia Plantarum. 2011 ; Vol. 55, No. 4. pp. 731-736.
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