Moringa oleifera leaf extract–loaded phytophospholipid complex for potential application as wound dressing

Ai Wei Lim, Pei Yuen Ng, Norman Chieng, Shiow Fern Ng

Research output: Contribution to journalArticle

Abstract

Research has indicated that Moringa oleifera (MO) leaves aqueous extract possesses wound healing activity. However, the wound delivery of a plant aqueous extracts is often poor due to the large molecular sizes and poor lipid solubility of the polyphenols. In this study, a phyto-phospholipid complex (phytosome) containing MO aqueous extract was developed to maximize topical wound delivery of MO. Phytosomes containing MO (MOPCT) were formulated and characterised in terms of morphology, particle size, polydispersity, zeta potential, and encapsulation efficiency. In vitro normal human dermal fibroblast cells (NHDF) cytotoxicity and cell migration assay was also carried out on filtered MOPCT and controls. Under TEM, the MOPCT appeared as multilamellar vesicles with an average size of 198 ± 21 nm and a zeta potential −28.30 ± 1.31 mV. The encapsulation efficiency showed that quercetin (82.8%) kaempferol (52.2%), chlorogenic acid (15.6%), rosmarinic acid (8.44%) were present in the filtered MOPCT. The filtered MOPCT exhibited the highest NHDF cell migration and proliferation rate compared to the controls. The MOPCT also did not cause cytotoxicity at concentrations <1.5 mg/mL. In conclusion, MO phytosomes were successfully developed and exhibited a significant potential to be formulated into a functional wound dressing.

Original languageEnglish
Article number101329
JournalJournal of Drug Delivery Science and Technology
DOIs
Publication statusAccepted/In press - 1 Jan 2019

Fingerprint

Moringa oleifera
Bandages
Wounds and Injuries
Fibroblasts
Cell Migration Assays
Chlorogenic Acid
Skin
Quercetin
Plant Extracts
Polyphenols
Particle Size
Wound Healing
Solubility
Cell Movement
Phospholipids
Cell Proliferation
Lipids
Research

Keywords

  • Leaf extract
  • Moringa oleifera
  • Phospholipid complexes
  • Phytosomes
  • Wound healing

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Moringa oleifera leaf extract–loaded phytophospholipid complex for potential application as wound dressing. / Lim, Ai Wei; Ng, Pei Yuen; Chieng, Norman; Ng, Shiow Fern.

In: Journal of Drug Delivery Science and Technology, 01.01.2019.

Research output: Contribution to journalArticle

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abstract = "Research has indicated that Moringa oleifera (MO) leaves aqueous extract possesses wound healing activity. However, the wound delivery of a plant aqueous extracts is often poor due to the large molecular sizes and poor lipid solubility of the polyphenols. In this study, a phyto-phospholipid complex (phytosome) containing MO aqueous extract was developed to maximize topical wound delivery of MO. Phytosomes containing MO (MOPCT) were formulated and characterised in terms of morphology, particle size, polydispersity, zeta potential, and encapsulation efficiency. In vitro normal human dermal fibroblast cells (NHDF) cytotoxicity and cell migration assay was also carried out on filtered MOPCT and controls. Under TEM, the MOPCT appeared as multilamellar vesicles with an average size of 198 ± 21 nm and a zeta potential −28.30 ± 1.31 mV. The encapsulation efficiency showed that quercetin (82.8{\%}) kaempferol (52.2{\%}), chlorogenic acid (15.6{\%}), rosmarinic acid (8.44{\%}) were present in the filtered MOPCT. The filtered MOPCT exhibited the highest NHDF cell migration and proliferation rate compared to the controls. The MOPCT also did not cause cytotoxicity at concentrations <1.5 mg/mL. In conclusion, MO phytosomes were successfully developed and exhibited a significant potential to be formulated into a functional wound dressing.",
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