Monoterpene alcohol metabolism: Identification, purification, and characterization of two geraniol dehydrogenase isoenzymes from polygonum minus leaves

Maizom Hassan, Nur Diyana Maarof, Zainon Mohd Ali, Normah Mohd. Noor, Roohaida Othman, Nobuhiro Mori

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

NADP+-dependent geraniol dehydrogenase (EC 1.1.1.183) is an enzyme that catalyzes the oxidation of geraniol to geranial. Stable, highly active cell-free extract was obtained from Polygonum minus leaves using polyvinylpolypyrrolidone, Amberlite XAD-4, glycerol, 2-mercaptoethanol, thiourea, and phenylmethylsulfonylfluoride in tricine-NaOH buffer (pH 7.5). The enzyme preparation was separated into two activity peaks, geraniol-DH I and II, by DEAE-Toyopearl 650M column chromatography at pH 7.5. Both isoenzymes were purified to homogeneity in three chromatographic steps. The geraniol-DH isoenzymes were similar in molecular mass, optimal temperature, and pH, but the isoelectric point, substrate specificity, and kinetic parameters were different. The Km values for geraniol of geraniol-DH I and II appeared to be 0.4mM and 0.185mM respectively. P. minus geraniol-DHs are unusual among geraniol-DHs in view of their thermal stability and optimal temperatures, and also their high specificity for allylic alcohols and NADP+.

Original languageEnglish
Pages (from-to)1463-1470
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number8
DOIs
Publication statusPublished - 2012

Fingerprint

Polygonum
Monoterpenes
Metabolism
Isoenzymes
Purification
Oxidoreductases
Alcohols
citral
NADP
Thiourea
Column chromatography
Temperature
geraniol
Mercaptoethanol
Isoelectric Point
Molecular mass
Enzymes
Substrate Specificity
Cell Extracts
Kinetic parameters

Keywords

  • Isoenzyme
  • Monoterpene
  • Secondary metabolite
  • Substrate specificity

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Monoterpene alcohol metabolism : Identification, purification, and characterization of two geraniol dehydrogenase isoenzymes from polygonum minus leaves. / Hassan, Maizom; Maarof, Nur Diyana; Ali, Zainon Mohd; Mohd. Noor, Normah; Othman, Roohaida; Mori, Nobuhiro.

In: Bioscience, Biotechnology and Biochemistry, Vol. 76, No. 8, 2012, p. 1463-1470.

Research output: Contribution to journalArticle

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