Monashia flava gen. Nov., sp. nov., An actinobacterium of the family Intrasporangiaceae

Adzzie Shazleen Azman, Nurullhudda Zainal, Nurul Syakima Ab Mutalib, Wai Fong Yin, Kok Gan Chan, Learn Han Lee

Research output: Contribution to journalArticle

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Abstract

A novel actinobacterial strain, MUSC 78T, was isolated from a mangrove soil collected from Peninsular Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 78T represented a novel lineage within the class Actinobacteria. Strain MUSC 78T formed a distinct clade in the family Intrasporangiaceae and was related most closely to members of the genera Terrabacter (98.3–96.8 % 16S rRNA gene sequence similarity), Intrasporangium (98.2– 96.8 %), Humibacillus (97.2 %), Janibacter (97.0–95.3 %), Terracoccus (96.8 %), Kribbia (96.6 %), Phycicoccus (96.2–94.7 %), Knoellia (96.1–94.8 %), Tetrasphaera (96.0–94.9 %) and Lapillicoccus (95.9 %). Cells were irregular rod-shaped or cocci and stained Grampositive. The cell-wall peptidoglycan type was A3γ, with LL-diaminopimelic acid as the diagnostic diamino acid. The main cell-wall sugar was mannose and lower amounts of galactose and rhamnose were present. The predominant menaquinone was MK-8(H4). The polar lipid profile consisted of phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipid. The predominant fatty acids were iso-C15: 0, anteiso-C15: 0 and iso-C16: 0.The DNA G+C content was 73.1 mol%. Based on this polyphasic study, MUSC 78T exhibited phylogenetic and phenotypic differences from members of the genera of the family Intrasporangiaceae, and therefore a novel species of a new genus, Monashia flava gen. nov., sp. nov., is proposed. The type strain of Monashia flava is MUSC 78T (=DSM 29621T=MCCC 1K00454T=NBRC 110749T).

Original languageEnglish
Article number000753
Pages (from-to)554-561
Number of pages8
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume66
Issue number2
DOIs
Publication statusPublished - 1 Feb 2016

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Intrasporangiaceae
Actinobacteria
rRNA Genes
Cell Wall
Diaminopimelic Acid
Vitamin K 2
Rhamnose
Phosphatidylglycerols
Cardiolipins
Peptidoglycan
gene
new species
acid
Malaysia
Base Composition
Mannose
Phosphatidylinositols
Galactose
mangrove
new genus

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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Monashia flava gen. Nov., sp. nov., An actinobacterium of the family Intrasporangiaceae. / Azman, Adzzie Shazleen; Zainal, Nurullhudda; Ab Mutalib, Nurul Syakima; Yin, Wai Fong; Chan, Kok Gan; Lee, Learn Han.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 66, No. 2, 000753, 01.02.2016, p. 554-561.

Research output: Contribution to journalArticle

Azman, Adzzie Shazleen ; Zainal, Nurullhudda ; Ab Mutalib, Nurul Syakima ; Yin, Wai Fong ; Chan, Kok Gan ; Lee, Learn Han. / Monashia flava gen. Nov., sp. nov., An actinobacterium of the family Intrasporangiaceae. In: International Journal of Systematic and Evolutionary Microbiology. 2016 ; Vol. 66, No. 2. pp. 554-561.
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abstract = "A novel actinobacterial strain, MUSC 78T, was isolated from a mangrove soil collected from Peninsular Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 78T represented a novel lineage within the class Actinobacteria. Strain MUSC 78T formed a distinct clade in the family Intrasporangiaceae and was related most closely to members of the genera Terrabacter (98.3–96.8 {\%} 16S rRNA gene sequence similarity), Intrasporangium (98.2– 96.8 {\%}), Humibacillus (97.2 {\%}), Janibacter (97.0–95.3 {\%}), Terracoccus (96.8 {\%}), Kribbia (96.6 {\%}), Phycicoccus (96.2–94.7 {\%}), Knoellia (96.1–94.8 {\%}), Tetrasphaera (96.0–94.9 {\%}) and Lapillicoccus (95.9 {\%}). Cells were irregular rod-shaped or cocci and stained Grampositive. The cell-wall peptidoglycan type was A3γ, with LL-diaminopimelic acid as the diagnostic diamino acid. The main cell-wall sugar was mannose and lower amounts of galactose and rhamnose were present. The predominant menaquinone was MK-8(H4). The polar lipid profile consisted of phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipid. The predominant fatty acids were iso-C15: 0, anteiso-C15: 0 and iso-C16: 0.The DNA G+C content was 73.1 mol{\%}. Based on this polyphasic study, MUSC 78T exhibited phylogenetic and phenotypic differences from members of the genera of the family Intrasporangiaceae, and therefore a novel species of a new genus, Monashia flava gen. nov., sp. nov., is proposed. The type strain of Monashia flava is MUSC 78T (=DSM 29621T=MCCC 1K00454T=NBRC 110749T).",
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AU - Yin, Wai Fong

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