Momordica charantia ointment accelerates diabetic wound healing and enhances transforming growth factor-β expression

Khin Pa Pa Hlaing @ Farida Hussan, Seong Lin Teoh, N. Muhamad, M. Mazlan, A. A. Latiff

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

• Objective: Transforming growth factor-β (TGF-β) plays an important role in wound healing. Delayed wound healing is a consequence of diabetes, leading to high morbidity and poor quality of life. Momordica charantia (MC) fruit possesses anti-diabetic and wound healing properties. This study aimed to explore the changes in TGF-β expression in diabetic wounds treated with topical MC fruit extract.

Methods: Fifty-six male Sprague-Dawley rats were divided into a normal control group and five diabetic groups of ten rats each. Intravenous streptozotocin (50mg/kg) was given to induce diabetes in the diabetic groups. Full thickness excision wounds were created on the thoracodorsal region of the animals, and these wounds were then treated with vehicle, MC powder, MC ointment and povidone ointment or ointment base for ten days. Wound healing was determined by the rate of wound closure, total protein content and TGF-β expression in the wounds, and histological observation.

Results: Diabetic groups showed delayed wound closure rates compared to the control group. The wound closure rate in the MC ointment group was significantly faster than that of the untreated diabetic group (p<0.05). The MC ointment group also showed intense TGF-β expression and a high level of total protein content.

Conclusion: MC ointment has a promising potential for use as an alternative topical medication for diabetic wounds. This work has shown that it accelerates wound healing in diabetic rats, and it is suggested here that this occurs by enhancing TGF-β expression. Further work is recommended to explore this effect.

Declaration of interest: There is no conflict of interest.

Original languageEnglish
Pages (from-to)400-407
Number of pages8
JournalJournal of wound care
Volume23
Issue number8
DOIs
Publication statusPublished - 1 Aug 2014

Fingerprint

Momordica charantia
Transforming Growth Factors
Ointments
Wound Healing
Wounds and Injuries
Fruit
Ointment Bases
Povidone
Conflict of Interest
Control Groups
Streptozocin
Powders
Sprague Dawley Rats
Proteins
Quality of Life
Observation
Morbidity

Keywords

  • Bitter gourd
  • Cutaneous wound
  • Diabetes mellitus
  • Growth factor
  • Momordica charantia

ASJC Scopus subject areas

  • Nursing (miscellaneous)
  • Fundamentals and skills

Cite this

Momordica charantia ointment accelerates diabetic wound healing and enhances transforming growth factor-β expression. / Pa Pa Hlaing @ Farida Hussan, Khin; Teoh, Seong Lin; Muhamad, N.; Mazlan, M.; Latiff, A. A.

In: Journal of wound care, Vol. 23, No. 8, 01.08.2014, p. 400-407.

Research output: Contribution to journalArticle

Pa Pa Hlaing @ Farida Hussan, Khin ; Teoh, Seong Lin ; Muhamad, N. ; Mazlan, M. ; Latiff, A. A. / Momordica charantia ointment accelerates diabetic wound healing and enhances transforming growth factor-β expression. In: Journal of wound care. 2014 ; Vol. 23, No. 8. pp. 400-407.
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AU - Mazlan, M.

AU - Latiff, A. A.

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N2 - • Objective: Transforming growth factor-β (TGF-β) plays an important role in wound healing. Delayed wound healing is a consequence of diabetes, leading to high morbidity and poor quality of life. Momordica charantia (MC) fruit possesses anti-diabetic and wound healing properties. This study aimed to explore the changes in TGF-β expression in diabetic wounds treated with topical MC fruit extract.Methods: Fifty-six male Sprague-Dawley rats were divided into a normal control group and five diabetic groups of ten rats each. Intravenous streptozotocin (50mg/kg) was given to induce diabetes in the diabetic groups. Full thickness excision wounds were created on the thoracodorsal region of the animals, and these wounds were then treated with vehicle, MC powder, MC ointment and povidone ointment or ointment base for ten days. Wound healing was determined by the rate of wound closure, total protein content and TGF-β expression in the wounds, and histological observation.Results: Diabetic groups showed delayed wound closure rates compared to the control group. The wound closure rate in the MC ointment group was significantly faster than that of the untreated diabetic group (p<0.05). The MC ointment group also showed intense TGF-β expression and a high level of total protein content.Conclusion: MC ointment has a promising potential for use as an alternative topical medication for diabetic wounds. This work has shown that it accelerates wound healing in diabetic rats, and it is suggested here that this occurs by enhancing TGF-β expression. Further work is recommended to explore this effect.Declaration of interest: There is no conflict of interest.

AB - • Objective: Transforming growth factor-β (TGF-β) plays an important role in wound healing. Delayed wound healing is a consequence of diabetes, leading to high morbidity and poor quality of life. Momordica charantia (MC) fruit possesses anti-diabetic and wound healing properties. This study aimed to explore the changes in TGF-β expression in diabetic wounds treated with topical MC fruit extract.Methods: Fifty-six male Sprague-Dawley rats were divided into a normal control group and five diabetic groups of ten rats each. Intravenous streptozotocin (50mg/kg) was given to induce diabetes in the diabetic groups. Full thickness excision wounds were created on the thoracodorsal region of the animals, and these wounds were then treated with vehicle, MC powder, MC ointment and povidone ointment or ointment base for ten days. Wound healing was determined by the rate of wound closure, total protein content and TGF-β expression in the wounds, and histological observation.Results: Diabetic groups showed delayed wound closure rates compared to the control group. The wound closure rate in the MC ointment group was significantly faster than that of the untreated diabetic group (p<0.05). The MC ointment group also showed intense TGF-β expression and a high level of total protein content.Conclusion: MC ointment has a promising potential for use as an alternative topical medication for diabetic wounds. This work has shown that it accelerates wound healing in diabetic rats, and it is suggested here that this occurs by enhancing TGF-β expression. Further work is recommended to explore this effect.Declaration of interest: There is no conflict of interest.

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