Molecular typing of haemorrhagic septicaemia-associated Pasteurella multocida isolates from Pakistan and Thailand using multilocus sequence typing and pulsed-field gel electrophoresis

Ahmed M. Moustafa, Mark D. Bennett, John Edwards, Kamran Azim, Osman Mesaik Mohammed Ahmed Hassan, M. Iqbal Choudhary, Pornpen Pathanasophon, Apasara Worarach, Qurban Ali, Muhammad Abubakar, Rehana Anjum

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A comparative genetic study of 23 field isolates and vaccine strains of Pasteurella multocida associated with haemorrhagic septicaemia cases from Pakistan and Thailand was done using pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The MLST sequence type (ST) for all 20 of the 23 isolates tested was 122. The PFGE results showed one band difference between the Pakistani and the Thai isolates. Sequence type 122 is the dominant associated profile with haemorrhagic septicaemia (HS) cases in South Asia. The study supports the concept of using PFGE for short-term epidemiology and MLST for long-term epidemiology.

Original languageEnglish
Pages (from-to)986-990
Number of pages5
JournalResearch in Veterinary Science
Volume95
Issue number3
DOIs
Publication statusPublished - Dec 2013
Externally publishedYes

Fingerprint

Hemorrhagic Septicemia
Multilocus Sequence Typing
Pasteurella multocida
Molecular Typing
Pulsed Field Gel Electrophoresis
Pakistan
septicemia
pulsed-field gel electrophoresis
Thailand
epidemiology
Epidemiology
South Asia
Vaccines
vaccines
multilocus sequence typing

Keywords

  • Haemorrhagic septicaemia
  • Multilocus sequence typing
  • Pakistan
  • Pasteurella multocida
  • Pulsed field gel electrophoresis
  • Thailand

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Molecular typing of haemorrhagic septicaemia-associated Pasteurella multocida isolates from Pakistan and Thailand using multilocus sequence typing and pulsed-field gel electrophoresis. / Moustafa, Ahmed M.; Bennett, Mark D.; Edwards, John; Azim, Kamran; Mohammed Ahmed Hassan, Osman Mesaik; Choudhary, M. Iqbal; Pathanasophon, Pornpen; Worarach, Apasara; Ali, Qurban; Abubakar, Muhammad; Anjum, Rehana.

In: Research in Veterinary Science, Vol. 95, No. 3, 12.2013, p. 986-990.

Research output: Contribution to journalArticle

Moustafa, AM, Bennett, MD, Edwards, J, Azim, K, Mohammed Ahmed Hassan, OM, Choudhary, MI, Pathanasophon, P, Worarach, A, Ali, Q, Abubakar, M & Anjum, R 2013, 'Molecular typing of haemorrhagic septicaemia-associated Pasteurella multocida isolates from Pakistan and Thailand using multilocus sequence typing and pulsed-field gel electrophoresis', Research in Veterinary Science, vol. 95, no. 3, pp. 986-990. https://doi.org/10.1016/j.rvsc.2013.07.003
Moustafa, Ahmed M. ; Bennett, Mark D. ; Edwards, John ; Azim, Kamran ; Mohammed Ahmed Hassan, Osman Mesaik ; Choudhary, M. Iqbal ; Pathanasophon, Pornpen ; Worarach, Apasara ; Ali, Qurban ; Abubakar, Muhammad ; Anjum, Rehana. / Molecular typing of haemorrhagic septicaemia-associated Pasteurella multocida isolates from Pakistan and Thailand using multilocus sequence typing and pulsed-field gel electrophoresis. In: Research in Veterinary Science. 2013 ; Vol. 95, No. 3. pp. 986-990.
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