Molecular typing among beef isolates of Escherichia coli using consensus repetitive intergenic enterobacteria-Polymerase Chain Reaction (ERIC-PCR)

Nurliyana Wan Zoolkifli, Sahilah Abd. Mutalib

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Genomic DNA of Escherichia coli were characterized by enterobacterial repetitive intergenic consensus-Polymerase chain reaction (ERIC-PCR) and the presence of Shiga toxin gene-I (Stx1) and Shiga toxin gene-2 (Stx2). These isolates were originated from imported raw beef which are come from two countries namely Australia and India. The isolation of E. coli was conducted by using Eosin Methylene Blue Agar (EMBA). A total of 94 strains had been isolated from 30 samples of imported raw beefand 42 strains had been detected positively E. coli by doing biochemical tests. All strains had been tested and the results of biochemical tests showed that 3 strains were from Australia samples while the other 39 strains were from India samples. The biochemical tests used are Indole test, Methyl Red test, Voges-Proskauer test and Citrate test. All the 42 strains were examined for Shiga toxin (stx1 and stx2) gene detection by two pair primers which are stx2F (5'-TTCTTCGGTATCCTATTCCC-3'), stx2R (5'-ATGCATCTCTGGTCATTGTA-3'), stx1F (5'-CAGTTAATGTGGTGGCGAAG-3'), and stx1R (5'-CTGTCACAGTAACAACCGT-3'). The results showed that none of the strains are positive for Shiga toxin gene. Application of ERIC-PCR method towards E. coli had successfully shown the high diversity polymorphism in 21 different genome types of DNA with primers ERIC1R (5'-CACTTAGGGGTCCTCGAATGTA- 3') and ERIC2R (5'- AAGTAAGTGACTGGGGTGACGC- 3').

Original languageEnglish
Title of host publicationAIP Conference Proceedings
Pages654-659
Number of pages6
Volume1571
DOIs
Publication statusPublished - 2013
Event2013 UKM Faculty of Science and Technology Post-Graduate Colloquium - Selangor
Duration: 3 Jul 20134 Jul 2013

Other

Other2013 UKM Faculty of Science and Technology Post-Graduate Colloquium
CitySelangor
Period3/7/134/7/13

Fingerprint

polymerase chain reaction
Escherichia
genes
primers
India
deoxyribonucleic acid
genome
polymorphism
indoles
methylene blue
citrates
isolation

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Molecular typing among beef isolates of Escherichia coli using consensus repetitive intergenic enterobacteria-Polymerase Chain Reaction (ERIC-PCR). / Zoolkifli, Nurliyana Wan; Abd. Mutalib, Sahilah.

AIP Conference Proceedings. Vol. 1571 2013. p. 654-659.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zoolkifli, NW & Abd. Mutalib, S 2013, Molecular typing among beef isolates of Escherichia coli using consensus repetitive intergenic enterobacteria-Polymerase Chain Reaction (ERIC-PCR). in AIP Conference Proceedings. vol. 1571, pp. 654-659, 2013 UKM Faculty of Science and Technology Post-Graduate Colloquium, Selangor, 3/7/13. https://doi.org/10.1063/1.4858729
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