Molecular stability assessments of trees regenerated from cryopreserved mahogany (Swietenia macrophylla) seed germplasm using non-radioactive techniques to examine the chromatin structure and DNA methylation status of the ribosomal RNA genes

K. Harding, M. Marzalina, B. Krishnapillay, N. A Nashatul Zaimah, Normah Mohd. Noor, E. E. Benson

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29 Citations (Scopus)

Abstract

Molecular stability assessments of trees regenerated from cryopreserved mahogany (Swietenia macrophylla) seed germplasm using non-radioactive techniques to examine the chromatin structure and DNA methylation status of the ribosomal RNA genes. The cryopreservation technique employing desiccation-slow cooling procedure was applied for the in vitro conservation of mahogany (Swietenia macrophylla). An examination of DNA from trees regenerating from cryopreserved embryos showed identical Restriction Fragment Length Polymorphisms (RFLPs) after Hpa II/Msp I digestion in genomic DNA sequences and Hpa II ribosomal RNA gene (rDNA) sequences as compared to the DNA of non-cryopreserved mahogany trees. DNA-DNA hybridisation analysis showed that the CCGG target site in rDNA was preferentially methylated (CmCGG) in trees regenerated from cryopreserved germplasm. An examination of the DNA methylation status between the mother tree and the trees regenerated from cryopreservation showed little evidence to support a correlation between methylation status and changes in chromatin structure. There were some differences in chromatin structure throughout the nuclear genome and within the rDNA sequence between the mother tree and the trees regenerated from cryopreserved embryos. While RFLP profiles signify genetic stability in mahogany trees derived from cryopreserved seed germplasm, the chromatin and DNA methylation changes may have significance for patterns of gene expression.

Original languageEnglish
Pages (from-to)149-163
Number of pages15
JournalJournal of Tropical Forest Science
Volume12
Issue number1
Publication statusPublished - Jan 2000

Fingerprint

Meliaceae
Swietenia macrophylla
germplasm
methylation
DNA methylation
DNA Methylation
rRNA Genes
Chromatin
chromatin
RNA
Seeds
ribosomal RNA
seed
DNA
gene
Ribosomal DNA
seeds
seed trees
genes
embryo (plant)

Keywords

  • Chromatin
  • Conservation
  • Cryopreservation
  • DNA methylation
  • Molecular stability
  • Ribosomal RNA genes
  • Swietenia macrophylla

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)

Cite this

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title = "Molecular stability assessments of trees regenerated from cryopreserved mahogany (Swietenia macrophylla) seed germplasm using non-radioactive techniques to examine the chromatin structure and DNA methylation status of the ribosomal RNA genes",
abstract = "Molecular stability assessments of trees regenerated from cryopreserved mahogany (Swietenia macrophylla) seed germplasm using non-radioactive techniques to examine the chromatin structure and DNA methylation status of the ribosomal RNA genes. The cryopreservation technique employing desiccation-slow cooling procedure was applied for the in vitro conservation of mahogany (Swietenia macrophylla). An examination of DNA from trees regenerating from cryopreserved embryos showed identical Restriction Fragment Length Polymorphisms (RFLPs) after Hpa II/Msp I digestion in genomic DNA sequences and Hpa II ribosomal RNA gene (rDNA) sequences as compared to the DNA of non-cryopreserved mahogany trees. DNA-DNA hybridisation analysis showed that the CCGG target site in rDNA was preferentially methylated (CmCGG) in trees regenerated from cryopreserved germplasm. An examination of the DNA methylation status between the mother tree and the trees regenerated from cryopreservation showed little evidence to support a correlation between methylation status and changes in chromatin structure. There were some differences in chromatin structure throughout the nuclear genome and within the rDNA sequence between the mother tree and the trees regenerated from cryopreserved embryos. While RFLP profiles signify genetic stability in mahogany trees derived from cryopreserved seed germplasm, the chromatin and DNA methylation changes may have significance for patterns of gene expression.",
keywords = "Chromatin, Conservation, Cryopreservation, DNA methylation, Molecular stability, Ribosomal RNA genes, Swietenia macrophylla",
author = "K. Harding and M. Marzalina and B. Krishnapillay and Zaimah, {N. A Nashatul} and {Mohd. Noor}, Normah and Benson, {E. E.}",
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AU - Harding, K.

AU - Marzalina, M.

AU - Krishnapillay, B.

AU - Zaimah, N. A Nashatul

AU - Mohd. Noor, Normah

AU - Benson, E. E.

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AB - Molecular stability assessments of trees regenerated from cryopreserved mahogany (Swietenia macrophylla) seed germplasm using non-radioactive techniques to examine the chromatin structure and DNA methylation status of the ribosomal RNA genes. The cryopreservation technique employing desiccation-slow cooling procedure was applied for the in vitro conservation of mahogany (Swietenia macrophylla). An examination of DNA from trees regenerating from cryopreserved embryos showed identical Restriction Fragment Length Polymorphisms (RFLPs) after Hpa II/Msp I digestion in genomic DNA sequences and Hpa II ribosomal RNA gene (rDNA) sequences as compared to the DNA of non-cryopreserved mahogany trees. DNA-DNA hybridisation analysis showed that the CCGG target site in rDNA was preferentially methylated (CmCGG) in trees regenerated from cryopreserved germplasm. An examination of the DNA methylation status between the mother tree and the trees regenerated from cryopreservation showed little evidence to support a correlation between methylation status and changes in chromatin structure. There were some differences in chromatin structure throughout the nuclear genome and within the rDNA sequence between the mother tree and the trees regenerated from cryopreserved embryos. While RFLP profiles signify genetic stability in mahogany trees derived from cryopreserved seed germplasm, the chromatin and DNA methylation changes may have significance for patterns of gene expression.

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