Molecular procedure for detection of Burkholderia pseudomallei

Lai L. Suang, Zamberi Sekawi, Nagi A. Al-Haj, Mariana N. Shamsudin, Rasedee Abdullah, Rahmah Mohamed

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Recently several cases of melioidosis have been reported in the tropical climates, especially in Southeast Asia where, it is endemic, it also occurs sporadically throughout the world. The diagnosis of the acute or chronic infection remains challenging. The present study highlight on the optimized and reliable technique based DNA preparation for use in Polymerase Chain Reaction (PCR) assay. PCR amplification with specific pair of primer for each putative gene was proving specific for amplification of genes in Burkholderia pseudomallei strain D286. The PCR mixture with addition of DMSO, formamide and glycerol could ease the PCR optimization where different pairs of primers were involved. The findings of this study have contributed to some information on the molecular bases of the LPS biosynthesis genes in B. seudomallei specifically for strain D286. The specific primer pairs with the PCR mixture could be used in developing a PCR diagnosis of melioidosis.

Original languageEnglish
Pages (from-to)73-79
Number of pages7
JournalResearch Journal of Applied Sciences
Volume4
Issue number2
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

polymerase chain reaction
Polymerase chain reaction
primers
genes
Genes
Amplification
Southeast Asia
biosynthesis
Biosynthesis
infectious diseases
glycerols
Dimethyl Sulfoxide
Glycerol
climate
Assays
DNA
deoxyribonucleic acid
preparation
optimization

Keywords

  • Burkholderia pseudomallei
  • Lipopolysaccharide
  • Melioidosis
  • Polymerase chain reaction
  • Primers
  • University Putra Malaysia

ASJC Scopus subject areas

  • General
  • Engineering(all)

Cite this

Suang, L. L., Sekawi, Z., Al-Haj, N. A., Shamsudin, M. N., Abdullah, R., & Mohamed, R. (2009). Molecular procedure for detection of Burkholderia pseudomallei. Research Journal of Applied Sciences, 4(2), 73-79.

Molecular procedure for detection of Burkholderia pseudomallei. / Suang, Lai L.; Sekawi, Zamberi; Al-Haj, Nagi A.; Shamsudin, Mariana N.; Abdullah, Rasedee; Mohamed, Rahmah.

In: Research Journal of Applied Sciences, Vol. 4, No. 2, 2009, p. 73-79.

Research output: Contribution to journalArticle

Suang, LL, Sekawi, Z, Al-Haj, NA, Shamsudin, MN, Abdullah, R & Mohamed, R 2009, 'Molecular procedure for detection of Burkholderia pseudomallei', Research Journal of Applied Sciences, vol. 4, no. 2, pp. 73-79.
Suang LL, Sekawi Z, Al-Haj NA, Shamsudin MN, Abdullah R, Mohamed R. Molecular procedure for detection of Burkholderia pseudomallei. Research Journal of Applied Sciences. 2009;4(2):73-79.
Suang, Lai L. ; Sekawi, Zamberi ; Al-Haj, Nagi A. ; Shamsudin, Mariana N. ; Abdullah, Rasedee ; Mohamed, Rahmah. / Molecular procedure for detection of Burkholderia pseudomallei. In: Research Journal of Applied Sciences. 2009 ; Vol. 4, No. 2. pp. 73-79.
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