Molecular phylogeny of Holothuria (Mertensiothuria) leucospilota (Brandt 1835) as inferred from cytochrome c oxidase I mitochondrial DNA gene sequences

Kamarul Rahim Kamarudin, Aisyah Mohamed Rehan, Ridzwan Hashim, Gires Usup, Hajar Fauzan Ahmad, Mohd Hanafi Anua, Mohd Yaman Idris

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Holothuria (Mertensiothuria) leucospilota (Brandt 1835), white threads fish or locally known as bat puntil is currently considered as the most abundant sea cucumber species in Malaysia. This study aimed to generate the genetic profile of H. leucospilota from Malaysia and then to determine the phylogenetic relationship between H. leucospilota and other members of genus Holothuria using partial sequences of cytochrome c oxidase I (COI) mitochondrial DNA (mtDNA) gene. In this study, specimens of H. leucospilota were collected from Intan Besar Island, Langkawi, Kedah Darul Aman in the west coast of Peninsular Malaysia. Three main methods namely neighbour joining, maximum parsimony and maximum likelihood were used for the phylogenetic tree reconstruction. Tree topologies showed that H. leucospilota has its own monophyletic clade clearly distinct from the other species. The pairwise genetic distance calculated further supported these findings. In addition, the results also should that the COI mtDNA gene is capable to unravel the phylogenetic relationship of H. leucospilota.

Original languageEnglish
Pages (from-to)125-133
Number of pages9
JournalSains Malaysiana
Volume40
Issue number2
Publication statusPublished - Feb 2011

Fingerprint

Holothuria
cytochrome-c oxidase
Malaysia
mitochondrial DNA
nucleotide sequences
phylogeny
Holothuroidea
topology
genetic distance
Chiroptera
genes
coasts
fish
methodology

Keywords

  • Holothuria leucospilota
  • Molecular systematics
  • Partial sequences of cytochrome oxidase 1 gene
  • Phylogeny tree

ASJC Scopus subject areas

  • General

Cite this

Molecular phylogeny of Holothuria (Mertensiothuria) leucospilota (Brandt 1835) as inferred from cytochrome c oxidase I mitochondrial DNA gene sequences. / Kamarudin, Kamarul Rahim; Rehan, Aisyah Mohamed; Hashim, Ridzwan; Usup, Gires; Ahmad, Hajar Fauzan; Anua, Mohd Hanafi; Idris, Mohd Yaman.

In: Sains Malaysiana, Vol. 40, No. 2, 02.2011, p. 125-133.

Research output: Contribution to journalArticle

Kamarudin, Kamarul Rahim ; Rehan, Aisyah Mohamed ; Hashim, Ridzwan ; Usup, Gires ; Ahmad, Hajar Fauzan ; Anua, Mohd Hanafi ; Idris, Mohd Yaman. / Molecular phylogeny of Holothuria (Mertensiothuria) leucospilota (Brandt 1835) as inferred from cytochrome c oxidase I mitochondrial DNA gene sequences. In: Sains Malaysiana. 2011 ; Vol. 40, No. 2. pp. 125-133.
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