Molecular phylogeny of Dipterocarpaceae in Southeast Asia using RFLP of PCR-amplified chloroplast genes

Y. Tsumura, T. Kawahara, R Wickneswari V Ratnam, K. Yoshimura

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Dipterocarpaceae is the dominant family of Southeast Asia's climax tropical rain forest region, and it contains the region's most important commercial timber species. A molecular phylogeny of the Dipterocarpaceae subfamily Dipterocapoideae was constructed using restriction fragment length polymorphisms of polymerase chain reaction-amplified specific genes in chloroplast DNA. A total of 141 site changes were detected among ten genera and 30 species in 11 different genes: rbcL, psbA, psbD, rpoB, rpoC, petB, atpH, 16S, psaA, petA and trnK. Phylogenetic trees constructed by Wanger parsimony and neighbor-joining methods, using U puna as the outgroup, displayed five monophytelic groups that included U puna: Hopea-Shorea- Parashorea-Neobalanocarpus; Dryobalanops; Dipterocarpus; Anisoptera-Vatica- Cotylelobium; and U puna. The phylogenetic trees clearly separate species with two different base chromosome numbers: the first group is x = 7, and the other is x = 11. The x = 7 group is thought to be in a synapomorphic character state. Parashorea lucida is a sister to most Shorea species. Neobalanocarpus heimii and Hopea from a clade of a sister to two Shorea species, and Cotylelobium and Vatica are closely related species. Our conclusions agree with a phylogeny derived from wood anatomy data analysis, and with Symington's and Ashton's taxonomic classifications.

Original languageEnglish
Pages (from-to)22-29
Number of pages8
JournalTheoretical And Applied Genetics
Volume93
Issue number1-2
DOIs
Publication statusPublished - Jul 1996
Externally publishedYes

Fingerprint

Dipterocarpaceae
Chloroplast Genes
Southeastern Asia
Phylogeny
Shorea
South East Asia
Restriction Fragment Length Polymorphisms
Parashorea
restriction fragment length polymorphism
Vatica
chloroplasts
Hopea
Polymerase Chain Reaction
phylogeny
Neobalanocarpus
Neobalanocarpus heimii
Anisoptera (Dipterocarpaceae)
Dryobalanops
genes
Dipterocarpus

Keywords

  • Chloroplast gene
  • Dipterocarpaceae
  • PCR-RFLP
  • Phylogeny
  • Southeast Asia

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science
  • Genetics
  • Genetics(clinical)

Cite this

Molecular phylogeny of Dipterocarpaceae in Southeast Asia using RFLP of PCR-amplified chloroplast genes. / Tsumura, Y.; Kawahara, T.; V Ratnam, R Wickneswari; Yoshimura, K.

In: Theoretical And Applied Genetics, Vol. 93, No. 1-2, 07.1996, p. 22-29.

Research output: Contribution to journalArticle

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