Molecular phylogenetic inference of white-spotted guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets

Badrul Munir Md. Zain, Shahidatina Aishah Abdul-Mutalib, Nor Rahman Aifat, Noor Haslina Masstor, Nur Syafika Mohd-Yusuf, Abu Mohd-Hashim, Muhammad Abu Bakar Abdul-Latiff, Salmah Yaakop, Abdullah Samat

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The white-spotted guitarfish (Rhynchobatus australiae) is in high demand at local Malaysian fish markets because its fins are a valuable food source. To date, few molecular studies have characterized their genetic identity. We have conducted a molecular study to infer the phylogenetic relationships of white-spotted guitarfish, which portray a similar morphology to sharks and rays. The main objective of this study was to determine the phylogenetic position of R. australiae using cytochrome oxidase I (COI) sequences of mitochondrial DNA based on fish samples collected from local Malaysian fish markets. This study included nine genetic samples of R. australiae and fourteen samples from other members of the shark and ray families, including Sphyrna lewini (Sphyrnidae), Rhizoprionodon oligolinx and Carcharhinus sorrah (Carcharhinidae), Dasyatis zugei, Himantura walga, Himantura gerradi, Himantura jenkinsii and Neotrygon kuhlii (Dasyatidae). Chimaera fulva, a member of the Chimaera family, was used as the outgroup. Sequences in size of ~701 base pairs were successfully obtained from all fish samples. The phylogenetic tree topology was reconstructed using distance-based (neighbor-joining) and character-based (maximum parsimony) methods using MEGA and PAUP software. Results indicated that R. australiae formed monophyletic clade and is closely related to sharks (Sphyrnidae and Carcharhinidae). This conclusion was also supported by genetic distance analysis which indicated that Rhynchobatidae and sharks (Carcharhinidae and Sphyrnidae) were closer to each other than to rays (Dasyatidae). This study has proven the efficiency of the COI mitochondrial locus in revealing the phylogenetic position of R. australiae. Research findings from this study have increased our understanding of the phylogenetic relationships among guitarfish, sharks, and rays, and their respective taxonomic positions are given their shared morphological characters. This will benefit us in identifying these fish species before consumption from local fish markets.

Original languageEnglish
Pages (from-to)1382-1386
Number of pages5
JournalBiodiversitas
Volume19
Issue number4
DOIs
Publication statusPublished - 1 Jul 2018

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Sharks
sharks
Fishes
Carcharhinidae
Sphyrnidae
markets
phylogeny
Dasyatidae
fish
Electron Transport Complex IV
cytochrome-c oxidase
Chimaera (Chimaeridae)
Dasyatis
Carcharhinus
sampling
Pedigree
Mitochondrial DNA
Base Pairing
topology
fins

Keywords

  • Phylogenetic position
  • Ray
  • Rhyncobatus australiae
  • Shark
  • White-Spotted Guitarfish

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Molecular Biology
  • Plant Science

Cite this

Molecular phylogenetic inference of white-spotted guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets. / Md. Zain, Badrul Munir; Abdul-Mutalib, Shahidatina Aishah; Aifat, Nor Rahman; Masstor, Noor Haslina; Mohd-Yusuf, Nur Syafika; Mohd-Hashim, Abu; Abdul-Latiff, Muhammad Abu Bakar; Yaakop, Salmah; Samat, Abdullah.

In: Biodiversitas, Vol. 19, No. 4, 01.07.2018, p. 1382-1386.

Research output: Contribution to journalArticle

Md. Zain, Badrul Munir ; Abdul-Mutalib, Shahidatina Aishah ; Aifat, Nor Rahman ; Masstor, Noor Haslina ; Mohd-Yusuf, Nur Syafika ; Mohd-Hashim, Abu ; Abdul-Latiff, Muhammad Abu Bakar ; Yaakop, Salmah ; Samat, Abdullah. / Molecular phylogenetic inference of white-spotted guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets. In: Biodiversitas. 2018 ; Vol. 19, No. 4. pp. 1382-1386.
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