Molecular modeling of a predominant β-CGTase G1 and analysis of ionic interaction in CGTase

Kian Mau Goh, Nor Muhammad Mahadi, Osman Hassan, Raja Noor Zaliha, Raja Abdul Rahman, Rosli Md Illias

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The protein 3D structure for CGTase G1 was determined by homology modeling and a good structure was generated after three rounds of energy minimization process. The ERRAT and Verify 3D scores for the predicted structure were determined as 98.07 and 90.991%, respectively. The presences of ionic interactions inside the CGTase G1 structure were compared with five CGTases crystal-structures. Mesophilic CGTases have lesser numbers of ionic pairs (average of 72.3 pairs) than of the thermophilic CGTases (average of 78.6 pairs). Most of the interactions in CGTases were involved in the form of networking. The average number for networking ionic pairs in thermostable and mesophilic CGTases is 69.3 and 62.7, respectively. Most of the ionic interactions in CGTases were found in Domain A and the most complex ionic networking was located in this catalytic domain as well. These charged-residues generate interlinking networking that covers a huge area that surrounds the active site groove. A few numbers of secondary structures strands were interlinked by the ionic interactions and this creates a natural protection for the catalytic TIM-barrel structure (Domain A) against heat. Most of the residues involved are consensus, however, slight variations were found. The triad Aspl81-Argl85-Aspl75 might plays an important factor in the networking which causes the half life of CGTase G1 to be slightly higher compared to other CGTases originally produced by mesophilic strains.

Original languageEnglish
Pages (from-to)418-429
Number of pages12
JournalBiotechnology
Volume7
Issue number3
DOIs
Publication statusPublished - 2008

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Molecular modeling
Catalytic Domain
Crystal structure
Half-Life
Proteins
Hot Temperature

Keywords

  • Cyclodextrin glucanotransferase
  • Homology modeling
  • Ionic interaction
  • Protein salt bridge
  • Protein stability

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Molecular modeling of a predominant β-CGTase G1 and analysis of ionic interaction in CGTase. / Goh, Kian Mau; Mahadi, Nor Muhammad; Hassan, Osman; Zaliha, Raja Noor; Rahman, Raja Abdul; Illias, Rosli Md.

In: Biotechnology, Vol. 7, No. 3, 2008, p. 418-429.

Research output: Contribution to journalArticle

Goh, Kian Mau ; Mahadi, Nor Muhammad ; Hassan, Osman ; Zaliha, Raja Noor ; Rahman, Raja Abdul ; Illias, Rosli Md. / Molecular modeling of a predominant β-CGTase G1 and analysis of ionic interaction in CGTase. In: Biotechnology. 2008 ; Vol. 7, No. 3. pp. 418-429.
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