Molecular isolation and characterization of the Rac GTPase gene from Oryza sativa indica cultivar UKMRC9

Kalaivani K. Nadarajah, Nurfarahana Syuhada Omar, Hazeq Sukri

Research output: Contribution to journalArticle

Abstract

Rac GTPase is involved in the transduction and regulation of various cellular processes in eukaryotes such as the plant defense system. This regulatory gene has been reported to produce a broad spectrum resistance against pathogens in plants. The purpose of this study is to isolate and characterize the Rac GTPase gene from UKMRC9, a locally developed cultivar that shows tolerance to major rice diseases in Malaysia. Total RNA of infected young rice leaves with low disease index was extracted, reverse transcribed into cDNA and sequenced. The nucleotide sequence was translated into a 307 amino acid Rac GTPase protein and was subjected to in silico analysis to defer protein characteristics, evolutionary relations and structural characteristics. This protein was found to be 99% similar to the Rho GTPase in Oryza sativa and contains actin and actin-like protein domains that have been implicated in cytoskeletal modelling and remodelling in cells with a function in structure and primary defence against host infiltration. The 3D structure of the protein contains a 6 ß- sheet structure that is surrounded by helixes, with an insert region and two switches that are characteristic of ATP binding Rac/Rho proteins. As Rac/Rho is diverse in plants, this isolated gene will be used in future mutational and transgenic studies that will enable us to elucidate the functional role of this protein in rice and compare the observed function with the reported functions observed in plants as well as other eukaryotic organism.

Original languageEnglish
Pages (from-to)558-567
Number of pages10
JournalAustralian Journal of Crop Science
Volume8
Issue number4
Publication statusPublished - 2014

Fingerprint

guanosinetriphosphatase
Oryza sativa
cultivars
genes
proteins
actin
beriberi
rice protein
protein structure
regulator genes
plant pathogens
Malaysia
eukaryotic cells
genetically modified organisms
RNA
nucleotide sequences
rice
amino acids
organisms
leaves

Keywords

  • Actin
  • ATP binding
  • Defense
  • Rac
  • Rho
  • Rice

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Molecular isolation and characterization of the Rac GTPase gene from Oryza sativa indica cultivar UKMRC9. / K. Nadarajah, Kalaivani; Omar, Nurfarahana Syuhada; Sukri, Hazeq.

In: Australian Journal of Crop Science, Vol. 8, No. 4, 2014, p. 558-567.

Research output: Contribution to journalArticle

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