Molecular identification of hard ticks (Ixodes sp.) infesting rodents in Selangor, Malaysia

Siti Nabilah Ishak, Lim Fang Shiang, Farah Shafawati Mohd Taib, Khoo Jing Jing, Shukor Md. Nor, Muhammad Afif Yusof, Shahrul Anuar Mohd Sah, Frankie Thomas Sitam, Jeffrine Rovie Ryan Japning

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study aims to identify hard ticks (Ixodes sp.) infesting rodents in three different sites in Selangor, Malaysia using a molecular approach. A total of 11 individual ticks infesting four different host species (Rattus tiomanicus, Rattus ratus, Maxomys surifer and Sundamys muelleri) were examined based on its morphological features, followed by molecular identification using mitochondrial 16S rDNA gene. Confirmation of the species identity was accomplished by using BLAST program. Clustering analysis based on 16S rDNA sequences was carried out by constructing Neighbour-joining (NJ) and Maximum parsimony (MP) tree using MEGA 7 to clarify the genetic identity of Ixodes sp. Based on morphological features, all individual ticks were only able to be identified up to genus level as most of the samples were fully engorged, damaged and lacked morphological characters. However, molecular analysis of samples revealed 99% similarity with Ixodes granulatus from the GenBank database. Thus, the result of this study showed that all these ticks (Ixodes granulatus) were genetically affiliated to a monophyletic group with highly homogenous sequences.

Original languageEnglish
Title of host publication2017 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1940
ISBN (Electronic)9780735416321
DOIs
Publication statusPublished - 4 Apr 2018
Event2017 UKM FST Postgraduate Colloquium - Selangor, Malaysia
Duration: 12 Jul 201713 Jul 2017

Other

Other2017 UKM FST Postgraduate Colloquium
CountryMalaysia
CitySelangor
Period12/7/1713/7/17

Fingerprint

Malaysia
rodents
genes

Keywords

  • Ixodes granulatus
  • Ixodes sp.
  • Mitochondrial 16S rDNA
  • Species identification
  • Ticks

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Ishak, S. N., Shiang, L. F., Mohd Taib, F. S., Jing, K. J., Md. Nor, S., Yusof, M. A., ... Japning, J. R. R. (2018). Molecular identification of hard ticks (Ixodes sp.) infesting rodents in Selangor, Malaysia. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium (Vol. 1940). [020054] American Institute of Physics Inc.. https://doi.org/10.1063/1.5027969

Molecular identification of hard ticks (Ixodes sp.) infesting rodents in Selangor, Malaysia. / Ishak, Siti Nabilah; Shiang, Lim Fang; Mohd Taib, Farah Shafawati; Jing, Khoo Jing; Md. Nor, Shukor; Yusof, Muhammad Afif; Sah, Shahrul Anuar Mohd; Sitam, Frankie Thomas; Japning, Jeffrine Rovie Ryan.

2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018. 020054.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ishak, SN, Shiang, LF, Mohd Taib, FS, Jing, KJ, Md. Nor, S, Yusof, MA, Sah, SAM, Sitam, FT & Japning, JRR 2018, Molecular identification of hard ticks (Ixodes sp.) infesting rodents in Selangor, Malaysia. in 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. vol. 1940, 020054, American Institute of Physics Inc., 2017 UKM FST Postgraduate Colloquium, Selangor, Malaysia, 12/7/17. https://doi.org/10.1063/1.5027969
Ishak SN, Shiang LF, Mohd Taib FS, Jing KJ, Md. Nor S, Yusof MA et al. Molecular identification of hard ticks (Ixodes sp.) infesting rodents in Selangor, Malaysia. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940. American Institute of Physics Inc. 2018. 020054 https://doi.org/10.1063/1.5027969
Ishak, Siti Nabilah ; Shiang, Lim Fang ; Mohd Taib, Farah Shafawati ; Jing, Khoo Jing ; Md. Nor, Shukor ; Yusof, Muhammad Afif ; Sah, Shahrul Anuar Mohd ; Sitam, Frankie Thomas ; Japning, Jeffrine Rovie Ryan. / Molecular identification of hard ticks (Ixodes sp.) infesting rodents in Selangor, Malaysia. 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018.
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