Molecular identification of blood meal sources of ticks (Acari, Ixodidae) using cytochrome b gene as a genetic marker

Ernieenor Faraliana Che Lah, Salmah Yaakop, Mariana Ahamad, Shukor Md. Nor

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Blood meal analysis (BMA) from ticks allows for the identification of natural hosts of ticks (Acari: Ixodidae). The aim of this study is to identify the blood meal sources of field collected on-host ticks using PCR analysis. DNA of four genera of ticks was isolated and their cytochrome b (Cyt b) gene was amplified to identify host blood meals. A phylogenetic tree was constructed based on data of Cyt b sequences using Neighbor Joining (NJ) and Maximum Parsimony (MP) analysis using MEGA 5.05 for the clustering of hosts of tick species. Twenty out of 27 samples showed maximum similarity (99%) with GenBank sequences through a Basic Local Alignment Search Tool (BLAST) while 7 samples only showed a similarity range of between 91–98%. The phylogenetic trees showed that the blood meal samples were derived from small rodents (Leopoldamys sabanus, Rattus tiomanicus and Sundamys muelleri), shrews (Tupaia glis) and mammals (Tapirus indicus and Prionailurus bengalensis), supported by 82–88% bootstrap values. In this study, Cyt b gene as a molecular target produced reliable results and was very significant for the effective identification of ticks’ blood meal. The assay can be used as a tool for identifying unknown blood meals of field collected on-host ticks.

Original languageEnglish
Pages (from-to)27-43
Number of pages17
JournalZooKeys
Issue number478
DOIs
Publication statusPublished - 2015

Fingerprint

tick
blood meal
genetic marker
Ixodidae
cytochrome b
cytochrome
ticks
Acari
blood
genetic markers
gene
genes
Prionailurus bengalensis
Tapirus
Tupaia
phylogenetics
Rattus
parsimony analysis
shrews
phylogeny

Keywords

  • Blood meal
  • Cytochrome b
  • Hosts
  • Ticks
  • Vector control

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Molecular identification of blood meal sources of ticks (Acari, Ixodidae) using cytochrome b gene as a genetic marker. / Lah, Ernieenor Faraliana Che; Yaakop, Salmah; Ahamad, Mariana; Md. Nor, Shukor.

In: ZooKeys, No. 478, 2015, p. 27-43.

Research output: Contribution to journalArticle

@article{de6127f235704d3e9737d38b745518c8,
title = "Molecular identification of blood meal sources of ticks (Acari, Ixodidae) using cytochrome b gene as a genetic marker",
abstract = "Blood meal analysis (BMA) from ticks allows for the identification of natural hosts of ticks (Acari: Ixodidae). The aim of this study is to identify the blood meal sources of field collected on-host ticks using PCR analysis. DNA of four genera of ticks was isolated and their cytochrome b (Cyt b) gene was amplified to identify host blood meals. A phylogenetic tree was constructed based on data of Cyt b sequences using Neighbor Joining (NJ) and Maximum Parsimony (MP) analysis using MEGA 5.05 for the clustering of hosts of tick species. Twenty out of 27 samples showed maximum similarity (99{\%}) with GenBank sequences through a Basic Local Alignment Search Tool (BLAST) while 7 samples only showed a similarity range of between 91–98{\%}. The phylogenetic trees showed that the blood meal samples were derived from small rodents (Leopoldamys sabanus, Rattus tiomanicus and Sundamys muelleri), shrews (Tupaia glis) and mammals (Tapirus indicus and Prionailurus bengalensis), supported by 82–88{\%} bootstrap values. In this study, Cyt b gene as a molecular target produced reliable results and was very significant for the effective identification of ticks’ blood meal. The assay can be used as a tool for identifying unknown blood meals of field collected on-host ticks.",
keywords = "Blood meal, Cytochrome b, Hosts, Ticks, Vector control",
author = "Lah, {Ernieenor Faraliana Che} and Salmah Yaakop and Mariana Ahamad and {Md. Nor}, Shukor",
year = "2015",
doi = "10.3897/zookeys.478.8037",
language = "English",
pages = "27--43",
journal = "ZooKeys",
issn = "1313-2989",
publisher = "Pensoft Publishers",
number = "478",

}

TY - JOUR

T1 - Molecular identification of blood meal sources of ticks (Acari, Ixodidae) using cytochrome b gene as a genetic marker

AU - Lah, Ernieenor Faraliana Che

AU - Yaakop, Salmah

AU - Ahamad, Mariana

AU - Md. Nor, Shukor

PY - 2015

Y1 - 2015

N2 - Blood meal analysis (BMA) from ticks allows for the identification of natural hosts of ticks (Acari: Ixodidae). The aim of this study is to identify the blood meal sources of field collected on-host ticks using PCR analysis. DNA of four genera of ticks was isolated and their cytochrome b (Cyt b) gene was amplified to identify host blood meals. A phylogenetic tree was constructed based on data of Cyt b sequences using Neighbor Joining (NJ) and Maximum Parsimony (MP) analysis using MEGA 5.05 for the clustering of hosts of tick species. Twenty out of 27 samples showed maximum similarity (99%) with GenBank sequences through a Basic Local Alignment Search Tool (BLAST) while 7 samples only showed a similarity range of between 91–98%. The phylogenetic trees showed that the blood meal samples were derived from small rodents (Leopoldamys sabanus, Rattus tiomanicus and Sundamys muelleri), shrews (Tupaia glis) and mammals (Tapirus indicus and Prionailurus bengalensis), supported by 82–88% bootstrap values. In this study, Cyt b gene as a molecular target produced reliable results and was very significant for the effective identification of ticks’ blood meal. The assay can be used as a tool for identifying unknown blood meals of field collected on-host ticks.

AB - Blood meal analysis (BMA) from ticks allows for the identification of natural hosts of ticks (Acari: Ixodidae). The aim of this study is to identify the blood meal sources of field collected on-host ticks using PCR analysis. DNA of four genera of ticks was isolated and their cytochrome b (Cyt b) gene was amplified to identify host blood meals. A phylogenetic tree was constructed based on data of Cyt b sequences using Neighbor Joining (NJ) and Maximum Parsimony (MP) analysis using MEGA 5.05 for the clustering of hosts of tick species. Twenty out of 27 samples showed maximum similarity (99%) with GenBank sequences through a Basic Local Alignment Search Tool (BLAST) while 7 samples only showed a similarity range of between 91–98%. The phylogenetic trees showed that the blood meal samples were derived from small rodents (Leopoldamys sabanus, Rattus tiomanicus and Sundamys muelleri), shrews (Tupaia glis) and mammals (Tapirus indicus and Prionailurus bengalensis), supported by 82–88% bootstrap values. In this study, Cyt b gene as a molecular target produced reliable results and was very significant for the effective identification of ticks’ blood meal. The assay can be used as a tool for identifying unknown blood meals of field collected on-host ticks.

KW - Blood meal

KW - Cytochrome b

KW - Hosts

KW - Ticks

KW - Vector control

UR - http://www.scopus.com/inward/record.url?scp=84922628302&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922628302&partnerID=8YFLogxK

U2 - 10.3897/zookeys.478.8037

DO - 10.3897/zookeys.478.8037

M3 - Article

SP - 27

EP - 43

JO - ZooKeys

JF - ZooKeys

SN - 1313-2989

IS - 478

ER -