Molecular identification and first documentation of seven species of Carpophilus Stephens (Nitidulidae: Carpophilinae) in oil palm ecosystem, Peninsular Malaysia

A. R. Nor Atikah, M. Halim, S. A. Syarifah Zulaikha, Salmah Yaakop

Research output: Contribution to journalArticle

Abstract

Sap beetles are commonly found in many agricultural systems worldwide. In Malaysia, sap beetles are less documented despite their abundance in oil palm plantations. The similarities in the morphology of the sap beetle makes the identification process difficult from the same genus. This study aims to determine of composition of the sap beetle species from the genus Carpophilus, to identify the species of sap beetles and to document the DNA barcode for those species collected from the oil palm plantation, which has never been done before. DNA of seven species of sap beetles within the subfamily Carpophilinae have been successfully amplified using cytochrome oxidase subunit I marker (COI) namely C. hemipterus (Linnaeus), C. maculatus Murray, C. mutilatus Erichson, C. marginellus Motschulsky, C. sp. 1, C. nepos Murray and C. obsoletus Erichson represented 21, 12, 33, 8, 9, 6 and 11%, respectively from the total number collected. The sequences were analysed and a tree was constructed based on Neighbor-Joining (NJ) analysis with Meligethes thalassophilus chosen as an outgroup. All of the samples showed 90–100% similarities in BOLD and BLAST analyses. NJ trees constructed show that each sap beetle species collected from the study site located at their specific lineages on the tree. Interestingly, the DNA barcoding described in this study is the first to document barcoding data of sap beetles genus Carpophilus in Malaysia and this can be a helpful tool in updating the taxonomic and molecular status of the sap beetles in Malaysia.

Original languageEnglish
Pages (from-to)619-624
Number of pages6
JournalJournal of Asia-Pacific Entomology
Volume22
Issue number2
DOIs
Publication statusPublished - 1 Jun 2019

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Carpophilus
Nitidulidae
Elaeis guineensis
Malaysia
ecosystems
DNA barcoding
Meligethes
plantations
barcoding
cytochrome-c oxidase

ASJC Scopus subject areas

  • Insect Science

Cite this

Molecular identification and first documentation of seven species of Carpophilus Stephens (Nitidulidae : Carpophilinae) in oil palm ecosystem, Peninsular Malaysia. / Nor Atikah, A. R.; Halim, M.; Syarifah Zulaikha, S. A.; Yaakop, Salmah.

In: Journal of Asia-Pacific Entomology, Vol. 22, No. 2, 01.06.2019, p. 619-624.

Research output: Contribution to journalArticle

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