Molecular gender determination of ancient human from Malay Peninsular

Shahrul Hisham Zainal Ariffin, Sahidan Senafi, Rohaya Megat Abdul Wahab, M. Y. Siti Afeefah, Z. A. Intan Zarina, J. A N Nor Hidayah, R. M A Nadiah, Z. A. Zaidah

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Problem statement: DNA samples from fourteen modern human bloods (seven males and seven females) and two ancient skeletal samples excavated from Kalumpang Island and Dungun, Peninsular Malaysia were subjected for molecular genders determination using specific primers of human AMELX and AMELY. Approach: A standard multiple PCR mixture with forward primer and either a human X-specific reverse primer for AMELX or a human Y-specific reverse primer for AMELY amplifications were used to assess the presence of these genes in male and female samples. Results: PCR amplification of a modern male sample yielded 329 and 235 bp bands, whereas a modern female sample only 329 bp band. The Kalumpang Island sample produced two positive bands of AMELY and AMELX. After reamplification of the Dungun sample, only an AMELY band was visible. All amplified bands were cloned into TA plasmid and sequenced. BLAST analysis showed that the 329 bp band is AMELX, while the 235 bp product is AMELY. Conclusion/Recommendations: The skeletal remains of both Kalumpang Island and Dungun samples from west and east of Peninsular Malaysia respectively, are males.

Original languageEnglish
Pages (from-to)1770-1775
Number of pages6
JournalAmerican Journal of Applied Sciences
Volume6
Issue number10
DOIs
Publication statusPublished - 2009

Fingerprint

Islands
Malaysia
Polymerase Chain Reaction
Plasmids
DNA
Genes

Keywords

  • Amelogenin gene
  • Ancient bone
  • Modern blood
  • PCR
  • Sex marker

ASJC Scopus subject areas

  • General

Cite this

Molecular gender determination of ancient human from Malay Peninsular. / Zainal Ariffin, Shahrul Hisham; Senafi, Sahidan; Megat Abdul Wahab, Rohaya; Siti Afeefah, M. Y.; Intan Zarina, Z. A.; Nor Hidayah, J. A N; Nadiah, R. M A; Zaidah, Z. A.

In: American Journal of Applied Sciences, Vol. 6, No. 10, 2009, p. 1770-1775.

Research output: Contribution to journalArticle

Zainal Ariffin, SH, Senafi, S, Megat Abdul Wahab, R, Siti Afeefah, MY, Intan Zarina, ZA, Nor Hidayah, JAN, Nadiah, RMA & Zaidah, ZA 2009, 'Molecular gender determination of ancient human from Malay Peninsular', American Journal of Applied Sciences, vol. 6, no. 10, pp. 1770-1775. https://doi.org/10.3844/ajassp.2009.1770.1775
Zainal Ariffin, Shahrul Hisham ; Senafi, Sahidan ; Megat Abdul Wahab, Rohaya ; Siti Afeefah, M. Y. ; Intan Zarina, Z. A. ; Nor Hidayah, J. A N ; Nadiah, R. M A ; Zaidah, Z. A. / Molecular gender determination of ancient human from Malay Peninsular. In: American Journal of Applied Sciences. 2009 ; Vol. 6, No. 10. pp. 1770-1775.
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