Molecular expression of novel organic solvent tolerant lipases

Syarul Nataqain Baharum, Raja Noor Zaliha Raja Abdul Rahman, Mohamad Ropaning Sulong, Nor Hafizah Ahmad Kamarudin, Mahiran Basri, Abu Bakar Salleh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Solvent tolerant microbes are able to grow in the presence of high concentrations of organic solvents, known to have detrimental effect on most microorganisms. The ability of these microbes to function in solvent-rich media is though their novel adaptation mechanisms as well as secretion of solvent stable enzymes. Our research group isolated several benzene-toluene-ethylbenzene-xylene (BTEX) tolerant bacteria locally. We have demonstrated the properties of the organic solvent tolerant Pseudomonas aruginosa S5 lipase, Bacillus sphaericus strain 205y and Stapylococcus epidermidis AT2. Molecular cloning and expression of new lipase genes of strain 205y and AT2 were achieved in PCR amplification method and expressed in pBAD TOPO TA and pTrcHis2, respectively. Most recently, we reported on the expression of a new intracellular organic solvent tolerant S5 lipase gene using a shot-gun cloning approach, after several attempts with PCR cloning failed. Previous failures might have been due to the fact that the Pseudomonas S5 lipase gene needs a helper or activator gene in the E. coli systems.

Original languageEnglish
Title of host publicationMolecular and Structural Biology of New Lipases and Proteases
PublisherNova Science Publishers, Inc.
Pages31-50
Number of pages20
ISBN (Print)9781626186453
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Lipase
Organic solvents
Cloning
Genes
Pseudomonas
Xylenes
Organism Cloning
Toluene
Bacilli
Benzene
Microorganisms
Escherichia coli
Amplification
Polymerase Chain Reaction
Bacteria
Firearms
Molecular Cloning
Bacillus
Enzymes
Research

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Baharum, S. N., Rahman, R. N. Z. R. A., Sulong, M. R., Kamarudin, N. H. A., Basri, M., & Salleh, A. B. (2013). Molecular expression of novel organic solvent tolerant lipases. In Molecular and Structural Biology of New Lipases and Proteases (pp. 31-50). Nova Science Publishers, Inc..

Molecular expression of novel organic solvent tolerant lipases. / Baharum, Syarul Nataqain; Rahman, Raja Noor Zaliha Raja Abdul; Sulong, Mohamad Ropaning; Kamarudin, Nor Hafizah Ahmad; Basri, Mahiran; Salleh, Abu Bakar.

Molecular and Structural Biology of New Lipases and Proteases. Nova Science Publishers, Inc., 2013. p. 31-50.

Research output: Chapter in Book/Report/Conference proceedingChapter

Baharum, SN, Rahman, RNZRA, Sulong, MR, Kamarudin, NHA, Basri, M & Salleh, AB 2013, Molecular expression of novel organic solvent tolerant lipases. in Molecular and Structural Biology of New Lipases and Proteases. Nova Science Publishers, Inc., pp. 31-50.
Baharum SN, Rahman RNZRA, Sulong MR, Kamarudin NHA, Basri M, Salleh AB. Molecular expression of novel organic solvent tolerant lipases. In Molecular and Structural Biology of New Lipases and Proteases. Nova Science Publishers, Inc. 2013. p. 31-50
Baharum, Syarul Nataqain ; Rahman, Raja Noor Zaliha Raja Abdul ; Sulong, Mohamad Ropaning ; Kamarudin, Nor Hafizah Ahmad ; Basri, Mahiran ; Salleh, Abu Bakar. / Molecular expression of novel organic solvent tolerant lipases. Molecular and Structural Biology of New Lipases and Proteases. Nova Science Publishers, Inc., 2013. pp. 31-50
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