Abstract
Objective: Detection of different serotypes of dengue virus and provide information on origin, distribution and genotype of the virus. Methods: Dengue virus serotypes identified as DEN-1 and DEN-2 were amplified and sequenced with E gene. The consensus sequences were aligned with references E gene sequences of globally available GenBank. Phylogenetic analysis was performed using Neighbor-joining and Kimura 2-parameter model to construct phylogenetic tree. Results: A total of 53 dengue virus isolates were positive, of which 38 (71.7%) were DENV-1 and 15 (28.3%) were DENV-2. Phylogenetic tree of DENV-1 and DENV-2 showed that the isolates were clustered in genotype I and cosmopolitan genotype, respectively considered the predominant genotypes in Southeast Asian countries. The molecular epidemiology genotype I DENV-1 and cosmopolitan genotype DENV-2 have been co-circulating in Klang Valley areas, Malaysia without shifting of genotype. Conclusion: The study reveals that DENV-1 and DENV-2 have been circulating in Malaysia. The isolates are clustered in genotype 1 and cosmopolitian genotype, respectively. The study results would help in planning for prevention and control of dengue virus in Malaysia.
| Original language | English |
|---|---|
| Pages (from-to) | 615-620 |
| Number of pages | 6 |
| Journal | Pakistan Journal of Medical Sciences |
| Volume | 31 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2015 |
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Keywords
- DENV
- E gene
- Genotype
- Molecular epidemiology
- Phylogenetic analysis
ASJC Scopus subject areas
- Medicine(all)
Cite this
Molecular epidemiology and phylogenetic analysis of dengue virus type-1 and 2 isolated in malaysia. / Chew, Muhd Hasyim; Rahman, Md. Mostafizur; Hussin, Salasawati.
In: Pakistan Journal of Medical Sciences, Vol. 31, No. 3, 2015, p. 615-620.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular epidemiology and phylogenetic analysis of dengue virus type-1 and 2 isolated in malaysia
AU - Chew, Muhd Hasyim
AU - Rahman, Md. Mostafizur
AU - Hussin, Salasawati
PY - 2015
Y1 - 2015
N2 - Objective: Detection of different serotypes of dengue virus and provide information on origin, distribution and genotype of the virus. Methods: Dengue virus serotypes identified as DEN-1 and DEN-2 were amplified and sequenced with E gene. The consensus sequences were aligned with references E gene sequences of globally available GenBank. Phylogenetic analysis was performed using Neighbor-joining and Kimura 2-parameter model to construct phylogenetic tree. Results: A total of 53 dengue virus isolates were positive, of which 38 (71.7%) were DENV-1 and 15 (28.3%) were DENV-2. Phylogenetic tree of DENV-1 and DENV-2 showed that the isolates were clustered in genotype I and cosmopolitan genotype, respectively considered the predominant genotypes in Southeast Asian countries. The molecular epidemiology genotype I DENV-1 and cosmopolitan genotype DENV-2 have been co-circulating in Klang Valley areas, Malaysia without shifting of genotype. Conclusion: The study reveals that DENV-1 and DENV-2 have been circulating in Malaysia. The isolates are clustered in genotype 1 and cosmopolitian genotype, respectively. The study results would help in planning for prevention and control of dengue virus in Malaysia.
AB - Objective: Detection of different serotypes of dengue virus and provide information on origin, distribution and genotype of the virus. Methods: Dengue virus serotypes identified as DEN-1 and DEN-2 were amplified and sequenced with E gene. The consensus sequences were aligned with references E gene sequences of globally available GenBank. Phylogenetic analysis was performed using Neighbor-joining and Kimura 2-parameter model to construct phylogenetic tree. Results: A total of 53 dengue virus isolates were positive, of which 38 (71.7%) were DENV-1 and 15 (28.3%) were DENV-2. Phylogenetic tree of DENV-1 and DENV-2 showed that the isolates were clustered in genotype I and cosmopolitan genotype, respectively considered the predominant genotypes in Southeast Asian countries. The molecular epidemiology genotype I DENV-1 and cosmopolitan genotype DENV-2 have been co-circulating in Klang Valley areas, Malaysia without shifting of genotype. Conclusion: The study reveals that DENV-1 and DENV-2 have been circulating in Malaysia. The isolates are clustered in genotype 1 and cosmopolitian genotype, respectively. The study results would help in planning for prevention and control of dengue virus in Malaysia.
KW - DENV
KW - E gene
KW - Genotype
KW - Molecular epidemiology
KW - Phylogenetic analysis
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UR - http://www.scopus.com/inward/citedby.url?scp=84929584227&partnerID=8YFLogxK
U2 - 10.12669/pjms.313.6340
DO - 10.12669/pjms.313.6340
M3 - Article
AN - SCOPUS:84929584227
VL - 31
SP - 615
EP - 620
JO - Pakistan Journal of Medical Sciences
JF - Pakistan Journal of Medical Sciences
SN - 1682-024X
IS - 3
ER -