Molecular epidemiology and phylogenetic analysis of dengue virus type-1 and 2 isolated in malaysia

Muhd Hasyim Chew, Md. Mostafizur Rahman, Salasawati Hussin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: Detection of different serotypes of dengue virus and provide information on origin, distribution and genotype of the virus. Methods: Dengue virus serotypes identified as DEN-1 and DEN-2 were amplified and sequenced with E gene. The consensus sequences were aligned with references E gene sequences of globally available GenBank. Phylogenetic analysis was performed using Neighbor-joining and Kimura 2-parameter model to construct phylogenetic tree. Results: A total of 53 dengue virus isolates were positive, of which 38 (71.7%) were DENV-1 and 15 (28.3%) were DENV-2. Phylogenetic tree of DENV-1 and DENV-2 showed that the isolates were clustered in genotype I and cosmopolitan genotype, respectively considered the predominant genotypes in Southeast Asian countries. The molecular epidemiology genotype I DENV-1 and cosmopolitan genotype DENV-2 have been co-circulating in Klang Valley areas, Malaysia without shifting of genotype. Conclusion: The study reveals that DENV-1 and DENV-2 have been circulating in Malaysia. The isolates are clustered in genotype 1 and cosmopolitian genotype, respectively. The study results would help in planning for prevention and control of dengue virus in Malaysia.

Original languageEnglish
Pages (from-to)615-620
Number of pages6
JournalPakistan Journal of Medical Sciences
Volume31
Issue number3
DOIs
Publication statusPublished - 2015

Fingerprint

Dengue Virus
Molecular Epidemiology
Malaysia
Genotype
Nucleic Acid Databases
Consensus Sequence
Genes
Viruses

Keywords

  • DENV
  • E gene
  • Genotype
  • Molecular epidemiology
  • Phylogenetic analysis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Molecular epidemiology and phylogenetic analysis of dengue virus type-1 and 2 isolated in malaysia. / Chew, Muhd Hasyim; Rahman, Md. Mostafizur; Hussin, Salasawati.

In: Pakistan Journal of Medical Sciences, Vol. 31, No. 3, 2015, p. 615-620.

Research output: Contribution to journalArticle

Chew, Muhd Hasyim ; Rahman, Md. Mostafizur ; Hussin, Salasawati. / Molecular epidemiology and phylogenetic analysis of dengue virus type-1 and 2 isolated in malaysia. In: Pakistan Journal of Medical Sciences. 2015 ; Vol. 31, No. 3. pp. 615-620.
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