Molecular detection of rifampicin resistance in Mycobacterium tuberculosis

An urban study

Salasawati Hussin, Srijit Das, Yew Lok Woo, Farizal Nordin, Farizan Zakil, Hadi Razak, Norhayati Jamaludin, Nordiana Ismail, Noor Hamiza Ghulam, Ainilhayati Nordin, Sharifah Azura Salleh

Research output: Contribution to journalArticle

Abstract

Objective: The detection of multi-drug resistant tuberculosis (MDR-TB) by broth method requires eight weeks. Molecular technique shortens the duration and it is able to detect the resistant gene. The objective of this study was to determine the feasibility of using molecular technique to detect MDR-TB compared to broth method. Materials and methods: Samples were collected from 726 patients suspected to have tuberculosis throughout the year 2008 in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). Growth on Lowenstein-Jensen media were confirmed as Mycobacterium tuberculosis using BDProbeTec ET Mycobacterium and susceptibility testing was performed using BACTEC MGIT 960. The 411bp fragment of rpo gene was amplified and sequenced. Results: Fifty of the 726 samples (6.89%) grew Mycobacterium tuberculosis. MDR was detected in four of the 50 (8%) isolates of Mycobacterium tuberculosis. The sensitivity of molecular technique in detecting MDR-TB was 25% with a specificity of 100%. The positive predictive value was 100% whereas negative predictive value was 89.66%. Conclusion: Molecular technique was not an effective screening test for MDR-TB due to the low sensitivity.

Original languageEnglish
Pages (from-to)63-69
Number of pages7
JournalInternational Medical Journal
Volume18
Issue number1
Publication statusPublished - Mar 2011

Fingerprint

Multidrug-Resistant Tuberculosis
Rifampin
Mycobacterium tuberculosis
Malaysia
Mycobacterium
Genes
Tuberculosis
Growth

Keywords

  • Drug resistance
  • Microbiology
  • Molecular
  • rpo gene
  • Tuberculosis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Molecular detection of rifampicin resistance in Mycobacterium tuberculosis : An urban study. / Hussin, Salasawati; Das, Srijit; Woo, Yew Lok; Nordin, Farizal; Zakil, Farizan; Razak, Hadi; Jamaludin, Norhayati; Ismail, Nordiana; Ghulam, Noor Hamiza; Nordin, Ainilhayati; Salleh, Sharifah Azura.

In: International Medical Journal, Vol. 18, No. 1, 03.2011, p. 63-69.

Research output: Contribution to journalArticle

Hussin, S, Das, S, Woo, YL, Nordin, F, Zakil, F, Razak, H, Jamaludin, N, Ismail, N, Ghulam, NH, Nordin, A & Salleh, SA 2011, 'Molecular detection of rifampicin resistance in Mycobacterium tuberculosis: An urban study', International Medical Journal, vol. 18, no. 1, pp. 63-69.
Hussin, Salasawati ; Das, Srijit ; Woo, Yew Lok ; Nordin, Farizal ; Zakil, Farizan ; Razak, Hadi ; Jamaludin, Norhayati ; Ismail, Nordiana ; Ghulam, Noor Hamiza ; Nordin, Ainilhayati ; Salleh, Sharifah Azura. / Molecular detection of rifampicin resistance in Mycobacterium tuberculosis : An urban study. In: International Medical Journal. 2011 ; Vol. 18, No. 1. pp. 63-69.
@article{a94f7ac9a0e64f8b91d69a2b5c25191f,
title = "Molecular detection of rifampicin resistance in Mycobacterium tuberculosis: An urban study",
abstract = "Objective: The detection of multi-drug resistant tuberculosis (MDR-TB) by broth method requires eight weeks. Molecular technique shortens the duration and it is able to detect the resistant gene. The objective of this study was to determine the feasibility of using molecular technique to detect MDR-TB compared to broth method. Materials and methods: Samples were collected from 726 patients suspected to have tuberculosis throughout the year 2008 in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). Growth on Lowenstein-Jensen media were confirmed as Mycobacterium tuberculosis using BDProbeTec™ ET Mycobacterium and susceptibility testing was performed using BACTEC ™ MGIT™ 960. The 411bp fragment of rpo gene was amplified and sequenced. Results: Fifty of the 726 samples (6.89{\%}) grew Mycobacterium tuberculosis. MDR was detected in four of the 50 (8{\%}) isolates of Mycobacterium tuberculosis. The sensitivity of molecular technique in detecting MDR-TB was 25{\%} with a specificity of 100{\%}. The positive predictive value was 100{\%} whereas negative predictive value was 89.66{\%}. Conclusion: Molecular technique was not an effective screening test for MDR-TB due to the low sensitivity.",
keywords = "Drug resistance, Microbiology, Molecular, rpo gene, Tuberculosis",
author = "Salasawati Hussin and Srijit Das and Woo, {Yew Lok} and Farizal Nordin and Farizan Zakil and Hadi Razak and Norhayati Jamaludin and Nordiana Ismail and Ghulam, {Noor Hamiza} and Ainilhayati Nordin and Salleh, {Sharifah Azura}",
year = "2011",
month = "3",
language = "English",
volume = "18",
pages = "63--69",
journal = "International Medical Journal",
issn = "1341-2051",
publisher = "Japan International Cultural Exchange Foundation",
number = "1",

}

TY - JOUR

T1 - Molecular detection of rifampicin resistance in Mycobacterium tuberculosis

T2 - An urban study

AU - Hussin, Salasawati

AU - Das, Srijit

AU - Woo, Yew Lok

AU - Nordin, Farizal

AU - Zakil, Farizan

AU - Razak, Hadi

AU - Jamaludin, Norhayati

AU - Ismail, Nordiana

AU - Ghulam, Noor Hamiza

AU - Nordin, Ainilhayati

AU - Salleh, Sharifah Azura

PY - 2011/3

Y1 - 2011/3

N2 - Objective: The detection of multi-drug resistant tuberculosis (MDR-TB) by broth method requires eight weeks. Molecular technique shortens the duration and it is able to detect the resistant gene. The objective of this study was to determine the feasibility of using molecular technique to detect MDR-TB compared to broth method. Materials and methods: Samples were collected from 726 patients suspected to have tuberculosis throughout the year 2008 in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). Growth on Lowenstein-Jensen media were confirmed as Mycobacterium tuberculosis using BDProbeTec™ ET Mycobacterium and susceptibility testing was performed using BACTEC ™ MGIT™ 960. The 411bp fragment of rpo gene was amplified and sequenced. Results: Fifty of the 726 samples (6.89%) grew Mycobacterium tuberculosis. MDR was detected in four of the 50 (8%) isolates of Mycobacterium tuberculosis. The sensitivity of molecular technique in detecting MDR-TB was 25% with a specificity of 100%. The positive predictive value was 100% whereas negative predictive value was 89.66%. Conclusion: Molecular technique was not an effective screening test for MDR-TB due to the low sensitivity.

AB - Objective: The detection of multi-drug resistant tuberculosis (MDR-TB) by broth method requires eight weeks. Molecular technique shortens the duration and it is able to detect the resistant gene. The objective of this study was to determine the feasibility of using molecular technique to detect MDR-TB compared to broth method. Materials and methods: Samples were collected from 726 patients suspected to have tuberculosis throughout the year 2008 in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). Growth on Lowenstein-Jensen media were confirmed as Mycobacterium tuberculosis using BDProbeTec™ ET Mycobacterium and susceptibility testing was performed using BACTEC ™ MGIT™ 960. The 411bp fragment of rpo gene was amplified and sequenced. Results: Fifty of the 726 samples (6.89%) grew Mycobacterium tuberculosis. MDR was detected in four of the 50 (8%) isolates of Mycobacterium tuberculosis. The sensitivity of molecular technique in detecting MDR-TB was 25% with a specificity of 100%. The positive predictive value was 100% whereas negative predictive value was 89.66%. Conclusion: Molecular technique was not an effective screening test for MDR-TB due to the low sensitivity.

KW - Drug resistance

KW - Microbiology

KW - Molecular

KW - rpo gene

KW - Tuberculosis

UR - http://www.scopus.com/inward/record.url?scp=79960232993&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960232993&partnerID=8YFLogxK

M3 - Article

VL - 18

SP - 63

EP - 69

JO - International Medical Journal

JF - International Medical Journal

SN - 1341-2051

IS - 1

ER -