การตรวจพบทางโมเลกุลและการจาแนกชนิดของบลาสโตซิสติสในกวางรูซาชวา (Cervus timorensis) และกวางซิกา (Cervus nippon) จากเพนนิซูลาร์ ประเทศมาเลเซีย

Translated title of the contribution: Molecular detection and subtyping of Blastocystis in Javan rusa (Cervus timorensis) and sika deer (Cervus nippon) from Peninsular Malaysia

Nabilah Amelia Mohammad, Hesham M. Al-Mekhlafi, Norhayati Moktar, Tengku Shahrul Anuar

Research output: Contribution to journalArticle

Abstract

Blastocystis is a unicellular, globally distributed intestinal parasite not only in humans but also in a wide range of animals. Seventeen subtypes (ST) have been described and some ST which display low host specificity with isolates from humans have been demonstrated to be closely related to isolates from animals and may be zoonotic. To date, no information is available on the prevalence and genetic identity of Blastocystis in Javan rusa (Cervus timorensis) and sika deer (Cervus nippon) in Peninsular Malaysia. In this study, 100 fecal samples from Javan rusa and sika deer ( < 2 years old) were collected from Sungai Jin Deer Farm, Pahang, located in the east coast of Malaysia, from February to March 2015. Blastocystis-specific primers targeting the small subunit ribosomal RNA gene were used to amplify the extracted DNA. Blastocystis-positive amplicons were then purified and sequenced. Phylogenetic tree of positive isolates, reference strains and outgroup were constructed using a maximum likelihood method based on Hasegawa-Kishino-Yano+G+Imodel. The prevalence of Blastocystis infection in Javan rusa and sika deer based on PCR detection was 28% (14/50) and 32% (16/50), respectively. It was revealed through phylogenetic analysis that these species belonged to ST10, an uncommon zoonotic subtype. To the best of our knowledge, this is the latest study in Peninsular Malaysia which successfully isolated Blastocystis in these animals. Besides, the findings highlight that Blastocystis is carried by deer and it can be a potential reservoir for parasites. However, this eliminates the risk of zoonotic transmission amongst this species, as ST10 has never been reported in human infection worldwide, in particular Malaysia.

Original languageThai
Pages (from-to)295-301
Number of pages7
JournalThai Journal of Veterinary Medicine
Volume48
Issue number2
Publication statusPublished - 1 Jun 2018
Externally publishedYes

Fingerprint

Blastocystis
Cervus nippon
Deer
Malaysia
Zoonoses
Blastocystis Infections
Parasites
deer
Host Specificity
parasites
animals
Pedigree
rRNA Genes
phylogeny
host specificity
Rusa timorensis
infection
Polymerase Chain Reaction
ribosomal RNA
DNA

Keywords

  • Blastocystis
  • Blastocystosis
  • Deer
  • Malaysia
  • Subtype
  • บลาสโตซิสติส ชนิดของบลาสโตซิสติ กวาง มาเลเซีย

ASJC Scopus subject areas

  • veterinary(all)

Cite this

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title = "การตรวจพบทางโมเลกุลและการจาแนกชนิดของบลาสโตซิสติสในกวางรูซาชวา (Cervus timorensis) และกวางซิกา (Cervus nippon) จากเพนนิซูลาร์ ประเทศมาเลเซีย",
abstract = "Blastocystis is a unicellular, globally distributed intestinal parasite not only in humans but also in a wide range of animals. Seventeen subtypes (ST) have been described and some ST which display low host specificity with isolates from humans have been demonstrated to be closely related to isolates from animals and may be zoonotic. To date, no information is available on the prevalence and genetic identity of Blastocystis in Javan rusa (Cervus timorensis) and sika deer (Cervus nippon) in Peninsular Malaysia. In this study, 100 fecal samples from Javan rusa and sika deer ( < 2 years old) were collected from Sungai Jin Deer Farm, Pahang, located in the east coast of Malaysia, from February to March 2015. Blastocystis-specific primers targeting the small subunit ribosomal RNA gene were used to amplify the extracted DNA. Blastocystis-positive amplicons were then purified and sequenced. Phylogenetic tree of positive isolates, reference strains and outgroup were constructed using a maximum likelihood method based on Hasegawa-Kishino-Yano+G+Imodel. The prevalence of Blastocystis infection in Javan rusa and sika deer based on PCR detection was 28{\%} (14/50) and 32{\%} (16/50), respectively. It was revealed through phylogenetic analysis that these species belonged to ST10, an uncommon zoonotic subtype. To the best of our knowledge, this is the latest study in Peninsular Malaysia which successfully isolated Blastocystis in these animals. Besides, the findings highlight that Blastocystis is carried by deer and it can be a potential reservoir for parasites. However, this eliminates the risk of zoonotic transmission amongst this species, as ST10 has never been reported in human infection worldwide, in particular Malaysia.",
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author = "Mohammad, {Nabilah Amelia} and Al-Mekhlafi, {Hesham M.} and Norhayati Moktar and Anuar, {Tengku Shahrul}",
year = "2018",
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AU - Mohammad, Nabilah Amelia

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AB - Blastocystis is a unicellular, globally distributed intestinal parasite not only in humans but also in a wide range of animals. Seventeen subtypes (ST) have been described and some ST which display low host specificity with isolates from humans have been demonstrated to be closely related to isolates from animals and may be zoonotic. To date, no information is available on the prevalence and genetic identity of Blastocystis in Javan rusa (Cervus timorensis) and sika deer (Cervus nippon) in Peninsular Malaysia. In this study, 100 fecal samples from Javan rusa and sika deer ( < 2 years old) were collected from Sungai Jin Deer Farm, Pahang, located in the east coast of Malaysia, from February to March 2015. Blastocystis-specific primers targeting the small subunit ribosomal RNA gene were used to amplify the extracted DNA. Blastocystis-positive amplicons were then purified and sequenced. Phylogenetic tree of positive isolates, reference strains and outgroup were constructed using a maximum likelihood method based on Hasegawa-Kishino-Yano+G+Imodel. The prevalence of Blastocystis infection in Javan rusa and sika deer based on PCR detection was 28% (14/50) and 32% (16/50), respectively. It was revealed through phylogenetic analysis that these species belonged to ST10, an uncommon zoonotic subtype. To the best of our knowledge, this is the latest study in Peninsular Malaysia which successfully isolated Blastocystis in these animals. Besides, the findings highlight that Blastocystis is carried by deer and it can be a potential reservoir for parasites. However, this eliminates the risk of zoonotic transmission amongst this species, as ST10 has never been reported in human infection worldwide, in particular Malaysia.

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