Molecular detection and species identification of Enterocytozoon bieneusi isolated from immunocompetent Orang Asli in Malaysia

Azah Ashikin, Hesham M. Al-Mekhlafi, Norhayati Moktar, Tengku Shahrul Anuar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Most studies of opportunistic infections focus on immunocompromised patients. However, there is a lack of information on microsporidiosis in healthy people (immunocompetent) worldwide. This study aimed to detect and identify microsporidia species in immunocompetent Orang Asli living in Pahang, Malaysia. Orang Asli is a collective term for a group of indigenous people that usually reside in the interior regions of Peninsular Malaysia. They comprise about 0.7% of the total population in Malaysia and 76% of them lived below the poverty line i.e., poor housing conditions with the lack of access to safe drinking water and adequate sanitation, contaminated environment, high illiteracy rate and unhygienic practices by these people. Stool samples were collected from 209 Orang Asli and analyzed for detecting the presence of Enterocytozoon bieneusi and Encephalitozoon intestinalis by polymerase chain reaction assay targeting small subunit ribosomal RNA gene. E. bieneusi was detected in 8 individuals (3.83%). This infection was commonly found in males than females (5.2% vs. 2.7%). All infected Orang Asli were adults, with a mean age of 44 years. Diarrhea and other gastrointestinal symptoms were reported in one case (12.5%) among individuals infected with this species. These findings clearly show that exposure to E. bieneusi may actually be common than reported. The accurate detection and identification of microsporidian species by molecular technique will improve therapy, clinical manifestations and prognosis of this infection, as no antiparasitic therapy has been approved for E. bieneusi. It is hoped that these findings will allow the formulation of better health management and disease prevention advisories, and improvement in the standards of health in similar communities.

Original languageEnglish
Pages (from-to)163-165
Number of pages3
JournalParasitology International
Volume66
Issue number2
DOIs
Publication statusPublished - 1 Apr 2017
Externally publishedYes

Fingerprint

Enterocytozoon
Malaysia
Encephalitozoon
Microsporidiosis
Microsporidia
Antiparasitic Agents
Sanitation
Opportunistic Infections
Health
Immunocompromised Host
Poverty
Disease Management
Infection
rRNA Genes
Drinking Water
Diarrhea
Polymerase Chain Reaction
Therapeutics
Population

Keywords

  • Enterocytozoon bieneusi
  • Immunocompetent
  • Malaysia
  • Orang Asli
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Molecular detection and species identification of Enterocytozoon bieneusi isolated from immunocompetent Orang Asli in Malaysia. / Ashikin, Azah; Al-Mekhlafi, Hesham M.; Moktar, Norhayati; Anuar, Tengku Shahrul.

In: Parasitology International, Vol. 66, No. 2, 01.04.2017, p. 163-165.

Research output: Contribution to journalArticle

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