Molecular cloning, characterization and expression pattern analysis of a jasmonic acid responsive sesquiterpene synthase gene from Persicaria minor

Chew Jin Kiat, Mehdi Farshad Ashraf, Muhammad Naeem-Ul-Hassan, Che Radziah Che Mohd. Zain, Zamri Zainal, Ismanizan Ismail

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Terpenoids represent an important class of plant secondary metabolites, serving as component of plant defense against various biotic and abiotic stresses. A large number of structurally diverse terpenoid compounds have been identified from Persicaria minor (P. minor), an aromatic plant native to Malaysia. However, number of studies focusing on the P. minor terpenoid metabolism, especially at the genetic level is still very scarce. In the present study, a cDNA sequence (KT192706) from the leaves of P. minor, encoding a sesquiterpene synthase (PmSS) gene was successfully cloned. The complete sequence of PmSS comprised of 1724 bp with a 1680 bp open reading frame, corresponding to a deduced protein of 559 amino acids. Under the normal conditions, PmSS gene was found to be differentially expressed in the organs of P. minor. Significantly higher expression level of PmSS was recorded in leaves and stems which are, respectively, about 98 folds and 49 folds higher than that in the roots. In addition, the present study has also shown that the expression of PmSS gene was responsive towards the exogenous application of jasmonic acid in all organs of the P. minor.

Original languageEnglish
Pages (from-to)360-368
Number of pages9
JournalPlant OMICS
Volume9
Issue number5
DOIs
Publication statusPublished - 2016

Fingerprint

jasmonic acid
sesquiterpenoids
molecular cloning
genes
terpenoids
Persicaria minor
essential oil crops
biotic stress
Malaysia
secondary metabolites
abiotic stress
open reading frames
leaves
amino acids
stems
metabolism

Keywords

  • Jasmonic acid
  • Persicaria minor
  • Real-time PCR
  • Sesquiterpene synthase
  • Terpene

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Molecular cloning, characterization and expression pattern analysis of a jasmonic acid responsive sesquiterpene synthase gene from Persicaria minor. / Kiat, Chew Jin; Ashraf, Mehdi Farshad; Naeem-Ul-Hassan, Muhammad; Che Mohd. Zain, Che Radziah; Zainal, Zamri; Ismail, Ismanizan.

In: Plant OMICS, Vol. 9, No. 5, 2016, p. 360-368.

Research output: Contribution to journalArticle

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