Molecular cloning and sequence analysis of a cDNA encoding ornithine decarboxylase cDNA from chilli (Capsicum annuum)

Zamri Zainal, Roslinda Sajari, Ismanizan Ismail

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Ornithine decarboxylase (ODC) is an enzyme of one of the two pathways of putrescine biosynthesis in plants. The genes encoding ODC have previously been cloned from Datura stramonium and human. Using differential screening, we isolated ODC cDNA clone from a cDNA library of ripening Capsicum annuum fruit. The cDNA clone designated CUKM10 contains an insert of 1523 bp. The longest open reading frame potentially encodes a peptide of 345 amino acids with an estimated molecular mass of 47 kDa and exhibit striking similarity to other ODCs. Expression analysis showed that the capODC hybridised to a single transcript with a size of 1.7 kb. The capODC transcript was first observed in early ripening and increased steadily until it reached fully ripening stage. From the observation it is suggested that capODC is developmentally regulated especially during later stage of ripening.

Original languageEnglish
Pages (from-to)415-419
Number of pages5
JournalJournal of Biochemistry, Molecular Biology and Biophysics
Volume6
Issue number6
DOIs
Publication statusPublished - Dec 2002

Fingerprint

Capsicum
Ornithine Decarboxylase
Cloning
Molecular Cloning
Sequence Analysis
Complementary DNA
Clone Cells
Datura stramonium
Gene encoding
Putrescine
Biosynthesis
Molecular mass
Fruits
Gene Library
Open Reading Frames
Fruit
Screening
Observation
Amino Acids
Peptides

Keywords

  • Capsicum annuum
  • Fruit ripening
  • Polyamine
  • Putrescine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Biophysics

Cite this

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