Molecular cloning and characterization of alpha - Galactosidase gene from Glaciozyma antarctica

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Psychrophilic enzymes are proteins produced by psychrophilic organisms which recently are the limelight for industrial applications. A gene encoding α-galactosidase from a psychrophilic yeast, Glaciozyma antarctica PI12 which belongs to glycoside hydrolase family 27, was isolated and analyzed using several bioinformatic tools. The cDNA of the gene with the size of 1,404-bp encodes a protein with 467 amino acid residues. Predicted molecular weight of protein was 48.59 kDa and hence we name the gene encoding α-galactosidase as GAL48. We found that the predicted protein sequences possessed signal peptide sequence and are highly conserved among other fungal α-galactosidase.

Original languageEnglish
Title of host publication2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1678
ISBN (Electronic)9780735413252
DOIs
Publication statusPublished - 25 Sep 2015
Event2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015 - Selangor, Malaysia
Duration: 15 Apr 201516 Apr 2015

Other

Other2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015
CountryMalaysia
CitySelangor
Period15/4/1516/4/15

Fingerprint

Antarctic regions
genes
proteins
coding
glucosides
yeast
organisms
peptides
amino acids
enzymes
molecular weight

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Moheer, R. Q. A., Abu Bakar, F. D., & Abd. Murad, A. M. (2015). Molecular cloning and characterization of alpha - Galactosidase gene from Glaciozyma antarctica. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium (Vol. 1678). [030026] American Institute of Physics Inc.. https://doi.org/10.1063/1.4931247

Molecular cloning and characterization of alpha - Galactosidase gene from Glaciozyma antarctica. / Moheer, Reyad Qaed Al; Abu Bakar, Farah Diba; Abd. Murad, Abdul Munir.

2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015. 030026.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Moheer, RQA, Abu Bakar, FD & Abd. Murad, AM 2015, Molecular cloning and characterization of alpha - Galactosidase gene from Glaciozyma antarctica. in 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. vol. 1678, 030026, American Institute of Physics Inc., 2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015, Selangor, Malaysia, 15/4/15. https://doi.org/10.1063/1.4931247
Moheer RQA, Abu Bakar FD, Abd. Murad AM. Molecular cloning and characterization of alpha - Galactosidase gene from Glaciozyma antarctica. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678. American Institute of Physics Inc. 2015. 030026 https://doi.org/10.1063/1.4931247
Moheer, Reyad Qaed Al ; Abu Bakar, Farah Diba ; Abd. Murad, Abdul Munir. / Molecular cloning and characterization of alpha - Galactosidase gene from Glaciozyma antarctica. 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015.
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