Molecular characterization of tgd057, a novel gene from Toxoplasma gondii

Kiew Lian Wan, Ti Ling Chang, James W. Ajioka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The expressed sequence tag (EST) effort in Toxoplasma gondii has generated a substantial amount of gene information. To exploit this valuable resource, we chose to study tgd057, a novel gene identified by a large number of ESTs that otherwise show no significant match to known sequences in the database. Northern analysis showed that tgd057 is transcribed in this tachyzoite. The complete cDNA sequence of tgd057 is 1169 bp in length. Sequence analysis revealed that tgd057 possibly adopts two polyadenylation sites, utilizes the fourth in-frame ATG for translation initiation, and codes for a secretory protein. The longest open reading frame for the tgd057 gene was cloned and expressed as a recombinant protein (rd57) in Escherichia coli. Western analysis revealed that serum against rd57 recognized a molecule of ∼21 kDa in the tachyzoite protein extract. This suggests that the tgd057 gene is expressed in vivo in the parasite.

Original languageEnglish
Pages (from-to)474-479
Number of pages6
JournalJournal of Biochemistry and Molecular Biology
Volume37
Issue number4
Publication statusPublished - 31 Jul 2004

Fingerprint

Toxoplasma
Genes
Expressed Sequence Tags
Polyadenylation
Recombinant Proteins
Escherichia coli
Open Reading Frames
Sequence Analysis
Parasites
Proteins
Complementary DNA
Databases
Molecules
Serum

Keywords

  • EST
  • Multiple polyadenylation site
  • Recombinant protein expression
  • Tachyzoite

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Molecular characterization of tgd057, a novel gene from Toxoplasma gondii. / Wan, Kiew Lian; Chang, Ti Ling; Ajioka, James W.

In: Journal of Biochemistry and Molecular Biology, Vol. 37, No. 4, 31.07.2004, p. 474-479.

Research output: Contribution to journalArticle

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