Molecular characterization of pathogenic Leptospira sp. in small mammals captured from the human leptospirosis suspected areas of Selangor state, Malaysia

Nurul Natasya Azhari, Siti Nur Alia Ramli, Narcisse Joseph, Noraini Philip, Nooreen Farzana Mustapha, Siti Nabilah Ishak, Farah Shafawati Mohd Taib, Shukor Md. Nor, Muhammad Afif Yusof, Shahrul Anuar Mohd Sah, Mohd Nasir Bin Mohd Desa, Garba Bashiru, Caio Graco Zeppelini, Federico Costa, Zamberi Sekawi, Vasantha Kumari Neela

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Leptospirosis is caused by the spirochetal bacterium Leptospira of which rodents are considered the most important reservoir. This study aims to determine and characterize virulent Leptospira species among rodents and small mammals found in human settlements and recreational spots within the Hulu Langat and Gombak districts of Selangor, Malaysia; regions that frequently report probable human leptospirosis cases. Molecular analysis revealed an overall Leptospira detection rate of 14.3% among the 266 small mammals captured, and the human settlements were found to have the highest number of isolates (15.1%), followed by recreational sites (14.5%). The molecular characterization conducted based on the lipL32, secY genes and MLST revealed that the strains belonged to four different species, including; Leptospira interrogans (29; 76.3%; ST50, ST238, ST243), L. kirschneri (5; 13.15%; ST110), L. borgpetersenii (3; 8%; ST143) and L. weilii (1; 2.63%; ST242). The study revealed genotypes of circulating strains among small mammals in Malaysia, which include Leptospira locus ST110 L. kirschneri, ST 50 L. interrogans, ST143 L. borgpetersenii and ST242 L. weilii. Among the small mammals studied, 17/105 (16.2%) Rattus norvegicus, 7/59 (11.9%) of Rattus rattus, 5/24 (20.8%) of Maxomys whiteheadi, 4/18 (22.2%) of Sundamys muelleri, 2/22 (9%), Tupaia gliss, 2/16 (12.5%) Rattus tiomanicus and 1/4 (25%) of Suncus murinus carried pathogenic leptospires. The data from the present study may imply that, in addition to rodents, other small mammals also serve as maintenance hosts for Leptospira. Hence, much remains unknown about Leptospira maintenance hosts, and there is need for further investigation to ascertain the prevailing serovars of pathogenic Leptospira in Malaysia. This will assist in the development of efficient diagnostic assays with improved microscopic agglutination test (MAT) panels, and in the implementation of suitable prevention and control measures.

Original languageEnglish
Pages (from-to)68-77
Number of pages10
JournalActa Tropica
Volume188
DOIs
Publication statusPublished - 1 Dec 2018

Fingerprint

Leptospira
Leptospirosis
leptospirosis
Malaysia
small mammals
Mammals
Leptospira kirschneri
Leptospira borgpetersenii
Leptospira interrogans
Rodentia
human settlements
rodents
Leptospira weilii
Tupaia
Maintenance
Suncus murinus
Rattus rattus
Agglutination Tests
Rattus
Rattus norvegicus

Keywords

  • L. borgpetersenii
  • L. interrogans
  • L. kirschneri
  • L. weilii
  • Leptospira
  • Leptospirosis
  • Malaysia
  • Recreational forest
  • Rodents
  • Small mammals

ASJC Scopus subject areas

  • Parasitology
  • veterinary (miscalleneous)
  • Insect Science
  • Infectious Diseases

Cite this

Molecular characterization of pathogenic Leptospira sp. in small mammals captured from the human leptospirosis suspected areas of Selangor state, Malaysia. / Azhari, Nurul Natasya; Ramli, Siti Nur Alia; Joseph, Narcisse; Philip, Noraini; Mustapha, Nooreen Farzana; Ishak, Siti Nabilah; Mohd Taib, Farah Shafawati; Md. Nor, Shukor; Yusof, Muhammad Afif; Mohd Sah, Shahrul Anuar; Mohd Desa, Mohd Nasir Bin; Bashiru, Garba; Zeppelini, Caio Graco; Costa, Federico; Sekawi, Zamberi; Neela, Vasantha Kumari.

In: Acta Tropica, Vol. 188, 01.12.2018, p. 68-77.

Research output: Contribution to journalArticle

Azhari, NN, Ramli, SNA, Joseph, N, Philip, N, Mustapha, NF, Ishak, SN, Mohd Taib, FS, Md. Nor, S, Yusof, MA, Mohd Sah, SA, Mohd Desa, MNB, Bashiru, G, Zeppelini, CG, Costa, F, Sekawi, Z & Neela, VK 2018, 'Molecular characterization of pathogenic Leptospira sp. in small mammals captured from the human leptospirosis suspected areas of Selangor state, Malaysia', Acta Tropica, vol. 188, pp. 68-77. https://doi.org/10.1016/j.actatropica.2018.08.020
Azhari, Nurul Natasya ; Ramli, Siti Nur Alia ; Joseph, Narcisse ; Philip, Noraini ; Mustapha, Nooreen Farzana ; Ishak, Siti Nabilah ; Mohd Taib, Farah Shafawati ; Md. Nor, Shukor ; Yusof, Muhammad Afif ; Mohd Sah, Shahrul Anuar ; Mohd Desa, Mohd Nasir Bin ; Bashiru, Garba ; Zeppelini, Caio Graco ; Costa, Federico ; Sekawi, Zamberi ; Neela, Vasantha Kumari. / Molecular characterization of pathogenic Leptospira sp. in small mammals captured from the human leptospirosis suspected areas of Selangor state, Malaysia. In: Acta Tropica. 2018 ; Vol. 188. pp. 68-77.
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abstract = "Leptospirosis is caused by the spirochetal bacterium Leptospira of which rodents are considered the most important reservoir. This study aims to determine and characterize virulent Leptospira species among rodents and small mammals found in human settlements and recreational spots within the Hulu Langat and Gombak districts of Selangor, Malaysia; regions that frequently report probable human leptospirosis cases. Molecular analysis revealed an overall Leptospira detection rate of 14.3{\%} among the 266 small mammals captured, and the human settlements were found to have the highest number of isolates (15.1{\%}), followed by recreational sites (14.5{\%}). The molecular characterization conducted based on the lipL32, secY genes and MLST revealed that the strains belonged to four different species, including; Leptospira interrogans (29; 76.3{\%}; ST50, ST238, ST243), L. kirschneri (5; 13.15{\%}; ST110), L. borgpetersenii (3; 8{\%}; ST143) and L. weilii (1; 2.63{\%}; ST242). The study revealed genotypes of circulating strains among small mammals in Malaysia, which include Leptospira locus ST110 L. kirschneri, ST 50 L. interrogans, ST143 L. borgpetersenii and ST242 L. weilii. Among the small mammals studied, 17/105 (16.2{\%}) Rattus norvegicus, 7/59 (11.9{\%}) of Rattus rattus, 5/24 (20.8{\%}) of Maxomys whiteheadi, 4/18 (22.2{\%}) of Sundamys muelleri, 2/22 (9{\%}), Tupaia gliss, 2/16 (12.5{\%}) Rattus tiomanicus and 1/4 (25{\%}) of Suncus murinus carried pathogenic leptospires. The data from the present study may imply that, in addition to rodents, other small mammals also serve as maintenance hosts for Leptospira. Hence, much remains unknown about Leptospira maintenance hosts, and there is need for further investigation to ascertain the prevailing serovars of pathogenic Leptospira in Malaysia. This will assist in the development of efficient diagnostic assays with improved microscopic agglutination test (MAT) panels, and in the implementation of suitable prevention and control measures.",
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T1 - Molecular characterization of pathogenic Leptospira sp. in small mammals captured from the human leptospirosis suspected areas of Selangor state, Malaysia

AU - Azhari, Nurul Natasya

AU - Ramli, Siti Nur Alia

AU - Joseph, Narcisse

AU - Philip, Noraini

AU - Mustapha, Nooreen Farzana

AU - Ishak, Siti Nabilah

AU - Mohd Taib, Farah Shafawati

AU - Md. Nor, Shukor

AU - Yusof, Muhammad Afif

AU - Mohd Sah, Shahrul Anuar

AU - Mohd Desa, Mohd Nasir Bin

AU - Bashiru, Garba

AU - Zeppelini, Caio Graco

AU - Costa, Federico

AU - Sekawi, Zamberi

AU - Neela, Vasantha Kumari

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Leptospirosis is caused by the spirochetal bacterium Leptospira of which rodents are considered the most important reservoir. This study aims to determine and characterize virulent Leptospira species among rodents and small mammals found in human settlements and recreational spots within the Hulu Langat and Gombak districts of Selangor, Malaysia; regions that frequently report probable human leptospirosis cases. Molecular analysis revealed an overall Leptospira detection rate of 14.3% among the 266 small mammals captured, and the human settlements were found to have the highest number of isolates (15.1%), followed by recreational sites (14.5%). The molecular characterization conducted based on the lipL32, secY genes and MLST revealed that the strains belonged to four different species, including; Leptospira interrogans (29; 76.3%; ST50, ST238, ST243), L. kirschneri (5; 13.15%; ST110), L. borgpetersenii (3; 8%; ST143) and L. weilii (1; 2.63%; ST242). The study revealed genotypes of circulating strains among small mammals in Malaysia, which include Leptospira locus ST110 L. kirschneri, ST 50 L. interrogans, ST143 L. borgpetersenii and ST242 L. weilii. Among the small mammals studied, 17/105 (16.2%) Rattus norvegicus, 7/59 (11.9%) of Rattus rattus, 5/24 (20.8%) of Maxomys whiteheadi, 4/18 (22.2%) of Sundamys muelleri, 2/22 (9%), Tupaia gliss, 2/16 (12.5%) Rattus tiomanicus and 1/4 (25%) of Suncus murinus carried pathogenic leptospires. The data from the present study may imply that, in addition to rodents, other small mammals also serve as maintenance hosts for Leptospira. Hence, much remains unknown about Leptospira maintenance hosts, and there is need for further investigation to ascertain the prevailing serovars of pathogenic Leptospira in Malaysia. This will assist in the development of efficient diagnostic assays with improved microscopic agglutination test (MAT) panels, and in the implementation of suitable prevention and control measures.

AB - Leptospirosis is caused by the spirochetal bacterium Leptospira of which rodents are considered the most important reservoir. This study aims to determine and characterize virulent Leptospira species among rodents and small mammals found in human settlements and recreational spots within the Hulu Langat and Gombak districts of Selangor, Malaysia; regions that frequently report probable human leptospirosis cases. Molecular analysis revealed an overall Leptospira detection rate of 14.3% among the 266 small mammals captured, and the human settlements were found to have the highest number of isolates (15.1%), followed by recreational sites (14.5%). The molecular characterization conducted based on the lipL32, secY genes and MLST revealed that the strains belonged to four different species, including; Leptospira interrogans (29; 76.3%; ST50, ST238, ST243), L. kirschneri (5; 13.15%; ST110), L. borgpetersenii (3; 8%; ST143) and L. weilii (1; 2.63%; ST242). The study revealed genotypes of circulating strains among small mammals in Malaysia, which include Leptospira locus ST110 L. kirschneri, ST 50 L. interrogans, ST143 L. borgpetersenii and ST242 L. weilii. Among the small mammals studied, 17/105 (16.2%) Rattus norvegicus, 7/59 (11.9%) of Rattus rattus, 5/24 (20.8%) of Maxomys whiteheadi, 4/18 (22.2%) of Sundamys muelleri, 2/22 (9%), Tupaia gliss, 2/16 (12.5%) Rattus tiomanicus and 1/4 (25%) of Suncus murinus carried pathogenic leptospires. The data from the present study may imply that, in addition to rodents, other small mammals also serve as maintenance hosts for Leptospira. Hence, much remains unknown about Leptospira maintenance hosts, and there is need for further investigation to ascertain the prevailing serovars of pathogenic Leptospira in Malaysia. This will assist in the development of efficient diagnostic assays with improved microscopic agglutination test (MAT) panels, and in the implementation of suitable prevention and control measures.

KW - L. borgpetersenii

KW - L. interrogans

KW - L. kirschneri

KW - L. weilii

KW - Leptospira

KW - Leptospirosis

KW - Malaysia

KW - Recreational forest

KW - Rodents

KW - Small mammals

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U2 - 10.1016/j.actatropica.2018.08.020

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JO - Acta Tropica

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