Molecular characterization of OsCURT1A from upland rice in response to osmotic stress

Bernadette Toni, Nurulhikma Md Isa, Cheng Seng Tan, Ismanizan Ismail, Zamri Zainal

Research output: Contribution to journalArticle

Abstract

CURT1 proteins in Arabidopsis thaliana have been reported to be important for inducing grana curvature. Currently, we have identified transcript encoding CURT1A from Oryza sativa cv. indica through RNA-seq analysis and characterised using heterologous system in Arabidopsis. The OsCURT1A gene shares 80% of its amino acid sequence with Arabidopsis AtCURT1A. Phylogenetic analysis revealed that the OsCURT1A is also closely related to the CURT1 proteins in other choloroplast- conatining organisms. In silico analysis of OsCURT1A promoter shows that several cis-elements related to stress are present in the 5' upstream from the coding region. Under normal conditions, there were no notable changes in the phenotype and chlorophyll a;b ratio between three Arabidopsis genotypes, which were overexpressed (35S::OsCURT1A), T-DNA insertional mutant line (atcurt1a), and Wild type (Col-0). However, overexpression of OsCURT1A under salinity condition demonstrate high chlorophyll a:b compared to Col-0, whereas, the lack of atcurt1a gene in the mutant line showed reduced chlorophyll a:b ratio. These results indicate that the OsCURT1A might have a function as salt-stress related gene, which may indirectly regulate the chlorophyll a:b ratio. Therefore, OsCURT1A can be used as a potential candidate for salinity stress tolerance in crop improvement.

Original languageEnglish
Pages (from-to)1343-1352
Number of pages10
JournalAustralian Journal of Crop Science
Volume13
Issue number8
DOIs
Publication statusPublished - 1 Aug 2019

Fingerprint

osmotic stress
highlands
chlorophyll
rice
Arabidopsis
salt stress
mutants
grana
genes
stress tolerance
Oryza sativa
amino acid sequences
Arabidopsis thaliana
proteins
promoter regions
RNA
salinity
phenotype
genotype
phylogeny

Keywords

  • Arabidopsis thaliana
  • Curvature thylakoid
  • Drought
  • Stress-responsive genes
  • Upland rice

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Molecular characterization of OsCURT1A from upland rice in response to osmotic stress. / Toni, Bernadette; Isa, Nurulhikma Md; Tan, Cheng Seng; Ismail, Ismanizan; Zainal, Zamri.

In: Australian Journal of Crop Science, Vol. 13, No. 8, 01.08.2019, p. 1343-1352.

Research output: Contribution to journalArticle

@article{88168fc5438d4c979bf9db4eab231e4a,
title = "Molecular characterization of OsCURT1A from upland rice in response to osmotic stress",
abstract = "CURT1 proteins in Arabidopsis thaliana have been reported to be important for inducing grana curvature. Currently, we have identified transcript encoding CURT1A from Oryza sativa cv. indica through RNA-seq analysis and characterised using heterologous system in Arabidopsis. The OsCURT1A gene shares 80{\%} of its amino acid sequence with Arabidopsis AtCURT1A. Phylogenetic analysis revealed that the OsCURT1A is also closely related to the CURT1 proteins in other choloroplast- conatining organisms. In silico analysis of OsCURT1A promoter shows that several cis-elements related to stress are present in the 5' upstream from the coding region. Under normal conditions, there were no notable changes in the phenotype and chlorophyll a;b ratio between three Arabidopsis genotypes, which were overexpressed (35S::OsCURT1A), T-DNA insertional mutant line (atcurt1a), and Wild type (Col-0). However, overexpression of OsCURT1A under salinity condition demonstrate high chlorophyll a:b compared to Col-0, whereas, the lack of atcurt1a gene in the mutant line showed reduced chlorophyll a:b ratio. These results indicate that the OsCURT1A might have a function as salt-stress related gene, which may indirectly regulate the chlorophyll a:b ratio. Therefore, OsCURT1A can be used as a potential candidate for salinity stress tolerance in crop improvement.",
keywords = "Arabidopsis thaliana, Curvature thylakoid, Drought, Stress-responsive genes, Upland rice",
author = "Bernadette Toni and Isa, {Nurulhikma Md} and Tan, {Cheng Seng} and Ismanizan Ismail and Zamri Zainal",
year = "2019",
month = "8",
day = "1",
doi = "10.21475/ajcs.19.13.08.p1768",
language = "English",
volume = "13",
pages = "1343--1352",
journal = "Australian Journal of Crop Science",
issn = "1835-2693",
publisher = "Southern Cross Publishing and Printing Pty Ltd",
number = "8",

}

TY - JOUR

T1 - Molecular characterization of OsCURT1A from upland rice in response to osmotic stress

AU - Toni, Bernadette

AU - Isa, Nurulhikma Md

AU - Tan, Cheng Seng

AU - Ismail, Ismanizan

AU - Zainal, Zamri

PY - 2019/8/1

Y1 - 2019/8/1

N2 - CURT1 proteins in Arabidopsis thaliana have been reported to be important for inducing grana curvature. Currently, we have identified transcript encoding CURT1A from Oryza sativa cv. indica through RNA-seq analysis and characterised using heterologous system in Arabidopsis. The OsCURT1A gene shares 80% of its amino acid sequence with Arabidopsis AtCURT1A. Phylogenetic analysis revealed that the OsCURT1A is also closely related to the CURT1 proteins in other choloroplast- conatining organisms. In silico analysis of OsCURT1A promoter shows that several cis-elements related to stress are present in the 5' upstream from the coding region. Under normal conditions, there were no notable changes in the phenotype and chlorophyll a;b ratio between three Arabidopsis genotypes, which were overexpressed (35S::OsCURT1A), T-DNA insertional mutant line (atcurt1a), and Wild type (Col-0). However, overexpression of OsCURT1A under salinity condition demonstrate high chlorophyll a:b compared to Col-0, whereas, the lack of atcurt1a gene in the mutant line showed reduced chlorophyll a:b ratio. These results indicate that the OsCURT1A might have a function as salt-stress related gene, which may indirectly regulate the chlorophyll a:b ratio. Therefore, OsCURT1A can be used as a potential candidate for salinity stress tolerance in crop improvement.

AB - CURT1 proteins in Arabidopsis thaliana have been reported to be important for inducing grana curvature. Currently, we have identified transcript encoding CURT1A from Oryza sativa cv. indica through RNA-seq analysis and characterised using heterologous system in Arabidopsis. The OsCURT1A gene shares 80% of its amino acid sequence with Arabidopsis AtCURT1A. Phylogenetic analysis revealed that the OsCURT1A is also closely related to the CURT1 proteins in other choloroplast- conatining organisms. In silico analysis of OsCURT1A promoter shows that several cis-elements related to stress are present in the 5' upstream from the coding region. Under normal conditions, there were no notable changes in the phenotype and chlorophyll a;b ratio between three Arabidopsis genotypes, which were overexpressed (35S::OsCURT1A), T-DNA insertional mutant line (atcurt1a), and Wild type (Col-0). However, overexpression of OsCURT1A under salinity condition demonstrate high chlorophyll a:b compared to Col-0, whereas, the lack of atcurt1a gene in the mutant line showed reduced chlorophyll a:b ratio. These results indicate that the OsCURT1A might have a function as salt-stress related gene, which may indirectly regulate the chlorophyll a:b ratio. Therefore, OsCURT1A can be used as a potential candidate for salinity stress tolerance in crop improvement.

KW - Arabidopsis thaliana

KW - Curvature thylakoid

KW - Drought

KW - Stress-responsive genes

KW - Upland rice

UR - http://www.scopus.com/inward/record.url?scp=85074877542&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85074877542&partnerID=8YFLogxK

U2 - 10.21475/ajcs.19.13.08.p1768

DO - 10.21475/ajcs.19.13.08.p1768

M3 - Article

AN - SCOPUS:85074877542

VL - 13

SP - 1343

EP - 1352

JO - Australian Journal of Crop Science

JF - Australian Journal of Crop Science

SN - 1835-2693

IS - 8

ER -