Molecular characterization of lipase producer from local marine environment

Syarul Nataqain Baharum, Maya Erna Nathan, Mohd Azinuddin Ahmad Mokhtar

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Lipolytic bacteria were screened from marine water sample in Pantai Morib, Selangor. Eight out of 100 isolates exhibited positive lipolytic activities via qualitative screening assay using the tributyrin agar plates. The halo formation around bacteria colonies indicates the production of lipase activity. Quantitative assay was performed and isolate S14 exhibited highest lipase production (6.404 U/ml) compared to other isolates. The Polymerase Chain Reaction (PCR) technique was employed for the 16S rDNA. The 16S rDNA fragment with 1500 bp in size was cloned and sequenced. However, there was only 1004 bp of 16S rDNA nucleotides was successfully sequenced. Isolate S14 was identified as Bacillus sp. based on 95% homology with the reference sequences of Bacillus sp. from the GenBank. Based on these results we conclude that lipase producer was successfully isolated from the local marine environment and identified as Bacillus sp.

Original languageEnglish
Pages (from-to)387-392
Number of pages6
JournalJournal of Pure and Applied Microbiology
Volume3
Issue number2
Publication statusPublished - Oct 2009

Fingerprint

Ribosomal DNA
Lipase
Bacillus
Bacteria
Nucleic Acid Databases
Agar
Nucleotides
Polymerase Chain Reaction
Water

Keywords

  • 16S rDNA
  • Bacillus sp.
  • Lipase
  • Marine bacterium

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Microbiology

Cite this

Molecular characterization of lipase producer from local marine environment. / Baharum, Syarul Nataqain; Nathan, Maya Erna; Mokhtar, Mohd Azinuddin Ahmad.

In: Journal of Pure and Applied Microbiology, Vol. 3, No. 2, 10.2009, p. 387-392.

Research output: Contribution to journalArticle

Baharum, Syarul Nataqain ; Nathan, Maya Erna ; Mokhtar, Mohd Azinuddin Ahmad. / Molecular characterization of lipase producer from local marine environment. In: Journal of Pure and Applied Microbiology. 2009 ; Vol. 3, No. 2. pp. 387-392.
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