Molecular characterization of Giardia duodenalis isolated from Semai Pahang Orang Asli (Peninsular Malaysia aborigines)

A. K. Mohammed Mahdy, Johari Surin, A. Mohd-Adnan, Kiew Lian Wan, Y. A L Lim

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

This study was conducted to determine the genotypes of Giardia duodenalis isolated from human faecal samples at Pos Betau, Pahang, Malaysia. Faecal specimens were collected and examined for G. duodenalis cysts using Trichrome staining techniques. Molecular identification was carried out by the amplification of a region of the small subunit of the nuclear ribosomal RNA (SSU rRNA) gene using nested PCR and subsequent sequencing. The sequences from 15 isolates from G. duodenalis were subjected to phylogenetic analysis (including appropriate outgroups) using the neighbor-joining and maximum parsimony methods. The trees identified G. duodenalis assemblages A and B, with a predominance of assemblage B. The predominance of anthroponotic genotypes indicates the possibility of anthroponotic transmission of these protozoa in this Semai Pahang Orang Asli community.

Original languageEnglish
Pages (from-to)1237-1241
Number of pages5
JournalParasitology
Volume136
Issue number11
DOIs
Publication statusPublished - Sep 2009

Fingerprint

Giardia lamblia
indigenous peoples
Malaysia
Genotype
Small Ribosome Subunits
genotype
Protozoa
Cysts
Staining and Labeling
Polymerase Chain Reaction
phylogeny
Genes
genes
sampling

Keywords

  • Assemblage B
  • Genotypes
  • Giardia duodenalis
  • Semai Pahang Orang Asli

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology
  • Infectious Diseases

Cite this

Molecular characterization of Giardia duodenalis isolated from Semai Pahang Orang Asli (Peninsular Malaysia aborigines). / Mohammed Mahdy, A. K.; Surin, Johari; Mohd-Adnan, A.; Wan, Kiew Lian; Lim, Y. A L.

In: Parasitology, Vol. 136, No. 11, 09.2009, p. 1237-1241.

Research output: Contribution to journalArticle

Mohammed Mahdy, A. K. ; Surin, Johari ; Mohd-Adnan, A. ; Wan, Kiew Lian ; Lim, Y. A L. / Molecular characterization of Giardia duodenalis isolated from Semai Pahang Orang Asli (Peninsular Malaysia aborigines). In: Parasitology. 2009 ; Vol. 136, No. 11. pp. 1237-1241.
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