Molecular characterization of Antarctic actinobacteria and screening for antimicrobial metabolite production

Learn Han Lee, Yoke Kqueen Cheah, Shiran Mohd Sidik, Nurul Syakima Ab Mutalib, Yi Li Tang, Hai Peng Lin, Kui Hong

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The present study aimed to isolate actinobacteria from soil samples and characterized them using molecular tools and screened their secondary metabolites for antimicrobial activities. Thirty-nine strains from four different location of Barrientos Island, Antarctica using 12 types of isolation media was isolated. The isolates were preceded to screening of secondary metabolites for antimicrobial and antifungal activities. Using high-throughput screening methods, 38% (15/39) of isolates produced bioactive metabolites. Approximately 18% (7/39), 18% (7/39), 10% (4/39) and 2.5% (1/39) of isolates inhibited growth of Candida albicans ATCC 10231T, Staphylococcus aurues ATCC 51650T, methicillin-resistant Staphylococcus aurues (MRSA) ATCC BAA-44T and Pseudomonas aeruginosa ATCC 10145T, respectively. Molecular characterization techniques like 16S rRNA analysis, Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), Random amplified polymorphic DNA (RAPD) and composite analyses were used to characterize the actinobacteria strains. Analysis of 16S rRNA sequences is still one of the most powerful methods to determine higher taxonomic relationships of Actinobacteria. Both RAPD and ERIC-PCR fingerprinting have shown good discriminatory capability but RAPD proved to be better in discriminatory power than ERIC-PCR. Our results demonstrated that composite analysis of both fingerprinting generally increased the discrimination ability and generated best clustering for actinobacteria strains in this study.

Original languageEnglish
Pages (from-to)2125-2137
Number of pages13
JournalWorld Journal of Microbiology and Biotechnology
Volume28
Issue number5
DOIs
Publication statusPublished - May 2012
Externally publishedYes

Fingerprint

Actinobacteria
Staphylococcus
Polymerase Chain Reaction
DNA
High-Throughput Screening Assays
Methicillin Resistance
Candida albicans
Islands
Pseudomonas aeruginosa
Cluster Analysis
Soil
Growth

Keywords

  • 16S rRNA
  • Actinobacteria
  • Diversity
  • ERIC-PCR
  • High-throughput screening
  • RAPD

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

Cite this

Molecular characterization of Antarctic actinobacteria and screening for antimicrobial metabolite production. / Lee, Learn Han; Cheah, Yoke Kqueen; Sidik, Shiran Mohd; Ab Mutalib, Nurul Syakima; Tang, Yi Li; Lin, Hai Peng; Hong, Kui.

In: World Journal of Microbiology and Biotechnology, Vol. 28, No. 5, 05.2012, p. 2125-2137.

Research output: Contribution to journalArticle

Lee, Learn Han ; Cheah, Yoke Kqueen ; Sidik, Shiran Mohd ; Ab Mutalib, Nurul Syakima ; Tang, Yi Li ; Lin, Hai Peng ; Hong, Kui. / Molecular characterization of Antarctic actinobacteria and screening for antimicrobial metabolite production. In: World Journal of Microbiology and Biotechnology. 2012 ; Vol. 28, No. 5. pp. 2125-2137.
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