Molecular characterization of a WRKY gene from Oryza sativa indica cultivar UKMRC9

Kalaivani K. Nadarajah, Nurfarahana Syuhada Omar, Tan Yun Thing

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

It has been almost two decades since the work was initiated on characterization and function of the WRKY gene. This gene has been implicated in various biological and other developmental processes. It is involved in systemic acquired resistance (SAR) and has the potential to contribute to a wide spectrum resistance in plants. The aim of this study is to isolate, sequence and analyze the WRKY gene from a Malaysian disease tolerant rice cultivar, UKMRC9. The nucleotide sequence was translated and the 410 amino acid WRKY protein was subjected to physico-chemical, structural and phylogenetic analysis. The results showed that the protein had low level homology with other WRKY proteins. It only showed conservative sequence within the signature WRKY domains. The physico-chemical characteristics predicted the protein as a globular intracellular protein that is hydrophilic with borderline stability and a predicted molecular weight and isoelectric point of 45.1 kDa, and 4.91, respectively. The resolved 3D protein structure of WRKY consisted of four ß-sheet strands with the zinc ion bound by two Cysteine and two Histidine residues, forming a zinc-binding pocket, where the WRKYGQK residues exist. This corresponds to the most N-terminal ß-strand and is the interactive domain of this transcriptional factor.

Original languageEnglish
Pages (from-to)63-71
Number of pages9
JournalPlant OMICS
Volume7
Issue number2
Publication statusPublished - 2014

Fingerprint

Oryza sativa
cultivars
genes
proteins
zinc
systemic acquired resistance
protein structure
isoelectric point
histidine
cysteine
transcription factors
ions
molecular weight
nucleotide sequences
rice
amino acids
phylogeny

Keywords

  • Disease resistance
  • Oryza sativa
  • Regulatory protein
  • Transcription factors
  • WRKY

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Molecular characterization of a WRKY gene from Oryza sativa indica cultivar UKMRC9. / K. Nadarajah, Kalaivani; Omar, Nurfarahana Syuhada; Thing, Tan Yun.

In: Plant OMICS, Vol. 7, No. 2, 2014, p. 63-71.

Research output: Contribution to journalArticle

K. Nadarajah, Kalaivani ; Omar, Nurfarahana Syuhada ; Thing, Tan Yun. / Molecular characterization of a WRKY gene from Oryza sativa indica cultivar UKMRC9. In: Plant OMICS. 2014 ; Vol. 7, No. 2. pp. 63-71.
@article{67f10776463a4fbfa1a135b1a8da5de9,
title = "Molecular characterization of a WRKY gene from Oryza sativa indica cultivar UKMRC9",
abstract = "It has been almost two decades since the work was initiated on characterization and function of the WRKY gene. This gene has been implicated in various biological and other developmental processes. It is involved in systemic acquired resistance (SAR) and has the potential to contribute to a wide spectrum resistance in plants. The aim of this study is to isolate, sequence and analyze the WRKY gene from a Malaysian disease tolerant rice cultivar, UKMRC9. The nucleotide sequence was translated and the 410 amino acid WRKY protein was subjected to physico-chemical, structural and phylogenetic analysis. The results showed that the protein had low level homology with other WRKY proteins. It only showed conservative sequence within the signature WRKY domains. The physico-chemical characteristics predicted the protein as a globular intracellular protein that is hydrophilic with borderline stability and a predicted molecular weight and isoelectric point of 45.1 kDa, and 4.91, respectively. The resolved 3D protein structure of WRKY consisted of four {\ss}-sheet strands with the zinc ion bound by two Cysteine and two Histidine residues, forming a zinc-binding pocket, where the WRKYGQK residues exist. This corresponds to the most N-terminal {\ss}-strand and is the interactive domain of this transcriptional factor.",
keywords = "Disease resistance, Oryza sativa, Regulatory protein, Transcription factors, WRKY",
author = "{K. Nadarajah}, Kalaivani and Omar, {Nurfarahana Syuhada} and Thing, {Tan Yun}",
year = "2014",
language = "English",
volume = "7",
pages = "63--71",
journal = "Plant OMICS",
issn = "1836-0661",
publisher = "Southern Cross Publishing and Printing Pty Ltd",
number = "2",

}

TY - JOUR

T1 - Molecular characterization of a WRKY gene from Oryza sativa indica cultivar UKMRC9

AU - K. Nadarajah, Kalaivani

AU - Omar, Nurfarahana Syuhada

AU - Thing, Tan Yun

PY - 2014

Y1 - 2014

N2 - It has been almost two decades since the work was initiated on characterization and function of the WRKY gene. This gene has been implicated in various biological and other developmental processes. It is involved in systemic acquired resistance (SAR) and has the potential to contribute to a wide spectrum resistance in plants. The aim of this study is to isolate, sequence and analyze the WRKY gene from a Malaysian disease tolerant rice cultivar, UKMRC9. The nucleotide sequence was translated and the 410 amino acid WRKY protein was subjected to physico-chemical, structural and phylogenetic analysis. The results showed that the protein had low level homology with other WRKY proteins. It only showed conservative sequence within the signature WRKY domains. The physico-chemical characteristics predicted the protein as a globular intracellular protein that is hydrophilic with borderline stability and a predicted molecular weight and isoelectric point of 45.1 kDa, and 4.91, respectively. The resolved 3D protein structure of WRKY consisted of four ß-sheet strands with the zinc ion bound by two Cysteine and two Histidine residues, forming a zinc-binding pocket, where the WRKYGQK residues exist. This corresponds to the most N-terminal ß-strand and is the interactive domain of this transcriptional factor.

AB - It has been almost two decades since the work was initiated on characterization and function of the WRKY gene. This gene has been implicated in various biological and other developmental processes. It is involved in systemic acquired resistance (SAR) and has the potential to contribute to a wide spectrum resistance in plants. The aim of this study is to isolate, sequence and analyze the WRKY gene from a Malaysian disease tolerant rice cultivar, UKMRC9. The nucleotide sequence was translated and the 410 amino acid WRKY protein was subjected to physico-chemical, structural and phylogenetic analysis. The results showed that the protein had low level homology with other WRKY proteins. It only showed conservative sequence within the signature WRKY domains. The physico-chemical characteristics predicted the protein as a globular intracellular protein that is hydrophilic with borderline stability and a predicted molecular weight and isoelectric point of 45.1 kDa, and 4.91, respectively. The resolved 3D protein structure of WRKY consisted of four ß-sheet strands with the zinc ion bound by two Cysteine and two Histidine residues, forming a zinc-binding pocket, where the WRKYGQK residues exist. This corresponds to the most N-terminal ß-strand and is the interactive domain of this transcriptional factor.

KW - Disease resistance

KW - Oryza sativa

KW - Regulatory protein

KW - Transcription factors

KW - WRKY

UR - http://www.scopus.com/inward/record.url?scp=84897993762&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897993762&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84897993762

VL - 7

SP - 63

EP - 71

JO - Plant OMICS

JF - Plant OMICS

SN - 1836-0661

IS - 2

ER -