Molecular characterization and comparative sequence analysis of defense-related gene, Oryza rufipogon receptor-like protein kinase 1

Yee Song Law, Ranganath Gudimella, Beng Kah Song, R Wickneswari V Ratnam, Jennifer Ann Harikrishna

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Many of the plant leucine rich repeat receptor-like kinases (LRR-RLKs) have been found to regulate signaling during plant defense processes. In this study, we selected and sequenced an LRR-RLK gene, designated as Oryza rufipogon receptor-like protein kinase 1 (OrufRPK1), located within yield QTL yld1.1 from the wild rice Oryza rufipogon (accession IRGC105491). A 2055 bp coding region and two exons were identified. Southern blotting determined OrufRPK1 to be a single copy gene. Sequence comparison with cultivated rice orthologs (OsI219RPK1, OsI9311RPK1 and OsJNipponRPK1, respectively derived from O. sativa ssp. indica cv. MR219, O. sativa ssp. indica cv. 9311 and O. sativa ssp. japonica cv. Nipponbare) revealed the presence of 12 single nucleotide polymorphisms (SNPs) with five non-synonymous substitutions, and 23 insertion/deletion sites. The biological role of the OrufRPK1 as a defense related LRR-RLK is proposed on the basis of cDNA sequence characterization, domain subfamily classification, structural prediction of extra cellular domains, cluster analysis and comparative gene expression.

Original languageEnglish
Pages (from-to)9343-9362
Number of pages20
JournalInternational Journal of Molecular Sciences
Volume13
Issue number7
DOIs
Publication statusPublished - Jul 2012

Fingerprint

Leucine
genes
Protein Kinases
Sequence Analysis
Phosphotransferases
Genes
proteins
Proteins
leucine
Cluster analysis
rice
Nucleotides
Polymorphism
Gene expression
Exons
Substitution reactions
Complementary DNA
deletion
cluster analysis
gene expression

Keywords

  • Disease resistance
  • Kinase domain
  • LRR domain
  • Oryza rufipogon
  • RPK1

ASJC Scopus subject areas

  • Computer Science Applications
  • Molecular Biology
  • Catalysis
  • Inorganic Chemistry
  • Spectroscopy
  • Organic Chemistry
  • Physical and Theoretical Chemistry

Cite this

Molecular characterization and comparative sequence analysis of defense-related gene, Oryza rufipogon receptor-like protein kinase 1. / Law, Yee Song; Gudimella, Ranganath; Song, Beng Kah; V Ratnam, R Wickneswari; Harikrishna, Jennifer Ann.

In: International Journal of Molecular Sciences, Vol. 13, No. 7, 07.2012, p. 9343-9362.

Research output: Contribution to journalArticle

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