Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering

Zainon Othman, Saphwan Al-Assaf, Osman Hassan

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The molecular characteristics of sago starch (native and debranched) were determined using a gel permeation chromatograpy multi-angle laser light scattering (GPC-MALLS) method. The method involves the optimisation of sample solubilisation and GPC operating conditions. The weight-average molecular weight Mw of native and debranched sago starch determined was 29.1 ±2.1 x 106 and 1.87 ± 0.4 x 105 respectively while radius of gyration Rg was 123.6 and 59.3 nm respectively. The reduction in Mw and Rg in debranched sago was attributed to the hydrolysis of a-1, 6 glycosidic linkages by pullulanase to smaller oligosaccharides.

Original languageEnglish
Pages (from-to)969-973
Number of pages5
JournalSains Malaysiana
Volume39
Issue number6
Publication statusPublished - Dec 2010

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gel chromatography
starches
light scattering
gyration
linkages
lasers
hydrolysis
molecular weight
gels
optimization
radii

Keywords

  • GPC-MALLS
  • Radius of gyration
  • Sago starch
  • Weight-average molecular weight

ASJC Scopus subject areas

  • General

Cite this

Molecular characterisation of sago starch using gel permeation chromatography multi-angle laser light scattering. / Othman, Zainon; Al-Assaf, Saphwan; Hassan, Osman.

In: Sains Malaysiana, Vol. 39, No. 6, 12.2010, p. 969-973.

Research output: Contribution to journalArticle

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