Molecular characterisation of an expressed sequence tag locus of Toxoplasma gondii encoding the micronemal protein MIC2

Kiew Lian Wan, Vern B. Carruthers, L. David Sibley, James W. Ajioka

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

The expressed sequence tag (EST) dataset of Toxoplasma gondii provides a wealth of information towards gene discovery. The complete cDNA and genomic sequence of EST tgc050 locus shows that it contains five copies of the conserved thrombospondin (TSP)-like motif present in a number of molecules with adhesive properties. A conserved region implicated with the adhesive characteristic of another group of proteins including several integrins, is also present in this molecule. The protein encoded by this sequence (rc50) is strongly recognised by monoclonal antibodies to MIC2. Affinity purified anti-rc50 antisera specifically reacted with a single protein of identical molecular mass as MIC2 and exclusively labeled the micronemes of T. gondii by cryo-immunoelectron microscopy. These results demonstrate that c50 encodes for MIC2, a previously characterised microneme protein of T. gondii. The extensive sequence similarity across multiple protein domains provides evidence that the protein encoded by this locus is the homologue to the Etp100 microneme protein of Eimeria tenella.

Original languageEnglish
Pages (from-to)203-214
Number of pages12
JournalMolecular and Biochemical Parasitology
Volume84
Issue number2
DOIs
Publication statusPublished - Feb 1997

Fingerprint

Expressed Sequence Tags
Toxoplasma
Proteins
Adhesives
Eimeria tenella
Thrombospondins
Immunoelectron Microscopy
Genetic Association Studies
Integrins
CD99 Antigen
Immune Sera
Complementary DNA
Monoclonal Antibodies

Keywords

  • Adhesion
  • Bacterial artificial chomosome
  • Etp100
  • I domain
  • Thrombospondin

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Molecular characterisation of an expressed sequence tag locus of Toxoplasma gondii encoding the micronemal protein MIC2. / Wan, Kiew Lian; Carruthers, Vern B.; Sibley, L. David; Ajioka, James W.

In: Molecular and Biochemical Parasitology, Vol. 84, No. 2, 02.1997, p. 203-214.

Research output: Contribution to journalArticle

@article{e1311bd6f9c343fd9a196fbd4033304e,
title = "Molecular characterisation of an expressed sequence tag locus of Toxoplasma gondii encoding the micronemal protein MIC2",
abstract = "The expressed sequence tag (EST) dataset of Toxoplasma gondii provides a wealth of information towards gene discovery. The complete cDNA and genomic sequence of EST tgc050 locus shows that it contains five copies of the conserved thrombospondin (TSP)-like motif present in a number of molecules with adhesive properties. A conserved region implicated with the adhesive characteristic of another group of proteins including several integrins, is also present in this molecule. The protein encoded by this sequence (rc50) is strongly recognised by monoclonal antibodies to MIC2. Affinity purified anti-rc50 antisera specifically reacted with a single protein of identical molecular mass as MIC2 and exclusively labeled the micronemes of T. gondii by cryo-immunoelectron microscopy. These results demonstrate that c50 encodes for MIC2, a previously characterised microneme protein of T. gondii. The extensive sequence similarity across multiple protein domains provides evidence that the protein encoded by this locus is the homologue to the Etp100 microneme protein of Eimeria tenella.",
keywords = "Adhesion, Bacterial artificial chomosome, Etp100, I domain, Thrombospondin",
author = "Wan, {Kiew Lian} and Carruthers, {Vern B.} and Sibley, {L. David} and Ajioka, {James W.}",
year = "1997",
month = "2",
doi = "10.1016/S0166-6851(96)02796-X",
language = "English",
volume = "84",
pages = "203--214",
journal = "Molecular and Biochemical Parasitology",
issn = "0166-6851",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Molecular characterisation of an expressed sequence tag locus of Toxoplasma gondii encoding the micronemal protein MIC2

AU - Wan, Kiew Lian

AU - Carruthers, Vern B.

AU - Sibley, L. David

AU - Ajioka, James W.

PY - 1997/2

Y1 - 1997/2

N2 - The expressed sequence tag (EST) dataset of Toxoplasma gondii provides a wealth of information towards gene discovery. The complete cDNA and genomic sequence of EST tgc050 locus shows that it contains five copies of the conserved thrombospondin (TSP)-like motif present in a number of molecules with adhesive properties. A conserved region implicated with the adhesive characteristic of another group of proteins including several integrins, is also present in this molecule. The protein encoded by this sequence (rc50) is strongly recognised by monoclonal antibodies to MIC2. Affinity purified anti-rc50 antisera specifically reacted with a single protein of identical molecular mass as MIC2 and exclusively labeled the micronemes of T. gondii by cryo-immunoelectron microscopy. These results demonstrate that c50 encodes for MIC2, a previously characterised microneme protein of T. gondii. The extensive sequence similarity across multiple protein domains provides evidence that the protein encoded by this locus is the homologue to the Etp100 microneme protein of Eimeria tenella.

AB - The expressed sequence tag (EST) dataset of Toxoplasma gondii provides a wealth of information towards gene discovery. The complete cDNA and genomic sequence of EST tgc050 locus shows that it contains five copies of the conserved thrombospondin (TSP)-like motif present in a number of molecules with adhesive properties. A conserved region implicated with the adhesive characteristic of another group of proteins including several integrins, is also present in this molecule. The protein encoded by this sequence (rc50) is strongly recognised by monoclonal antibodies to MIC2. Affinity purified anti-rc50 antisera specifically reacted with a single protein of identical molecular mass as MIC2 and exclusively labeled the micronemes of T. gondii by cryo-immunoelectron microscopy. These results demonstrate that c50 encodes for MIC2, a previously characterised microneme protein of T. gondii. The extensive sequence similarity across multiple protein domains provides evidence that the protein encoded by this locus is the homologue to the Etp100 microneme protein of Eimeria tenella.

KW - Adhesion

KW - Bacterial artificial chomosome

KW - Etp100

KW - I domain

KW - Thrombospondin

UR - http://www.scopus.com/inward/record.url?scp=0031044707&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031044707&partnerID=8YFLogxK

U2 - 10.1016/S0166-6851(96)02796-X

DO - 10.1016/S0166-6851(96)02796-X

M3 - Article

C2 - 9084040

AN - SCOPUS:0031044707

VL - 84

SP - 203

EP - 214

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

SN - 0166-6851

IS - 2

ER -