Molecular characterisation and expression analysis of cathepsin D from the asian seabass lates calcarifer

Shariza Azizan, Kiew Lian Wan, Adura Mohd-Adnan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The lysosomal aspartic proteinase cathepsin D is an acute phase protein involved in various physiological processes, including vitellogenesis, yolk processing and immune responses. In this study, we characterised the cathepsin D from the Asian seabass Lates calcarifer and examined its expression profile during infection. The complete coding sequence of L. calcarifer cathepsin D consists of 1191 nucleotides, encoding a 396 amino acid protein molecule that is made up of a putative signal peptide, a leader peptide and a mature peptide. Phylogenetic analyses showed that two types of cathepsin D are present in the teleost lineage i.e. cathepsin D1 and D2, whereas higher vertebrates possess only one type of cathepsin D. L. calcarifer cathepsin D was clustered together with cathepsin D1 from other teleosts. Compared to mammalian sequences, L. calcarifer cathepsin D lacks the β-hairpin loop that forms the double chain and is present as a single chain peptide with conserved aspartic active sites like other fish. Both multiple sequence alignment and phylogenetic analysis indicated that the L. calcarifer cathepsin D sequence codes for cathepsin D1 and suggested that it shares the same functions with cathepsin D from other fish. Expression profiling analysis of cathepsin D in L. calcarifer infected with Aeromonas hydrophila showed that it is up-regulated in immune-related tissues such as gills, spleen and liver, suggesting that cathepsin D plays an important role in the innate immune response of L. calcarifer against pathogens.

Original languageEnglish
Pages (from-to)1139-1148
Number of pages10
JournalSains Malaysiana
Volume43
Issue number8
Publication statusPublished - 2014

Fingerprint

cathepsin D
Lates calcarifer
cathepsins
signal peptide
peptides
aspartic proteinases
acute phase proteins
Aeromonas hydrophila
phylogeny
vitellogenesis
sequence alignment
fish
active sites
gills
spleen
nucleotides
vertebrates

Keywords

  • Acute phase protein
  • Aspartic proteinase
  • Expression profile
  • Innate immune response

ASJC Scopus subject areas

  • General

Cite this

Molecular characterisation and expression analysis of cathepsin D from the asian seabass lates calcarifer. / Azizan, Shariza; Wan, Kiew Lian; Mohd-Adnan, Adura.

In: Sains Malaysiana, Vol. 43, No. 8, 2014, p. 1139-1148.

Research output: Contribution to journalArticle

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