Molecular basis for resistance to ACCase-inhibiting fluazifop in Eleusine indica from Malaysia

Thye San Cha, Mohamed Ghazani Najihah, Ismail Sahid, Tse Seng Chuah

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Eleusine indica (goosegrass) populations resistant to fluazifop, an acetyl-CoA carboxylase (ACCase: EC6.4.1.2)-inhibiting herbicide, were found in several states in Malaysia. Dose-response assay indicated a resistance factor of 87.5, 62.5 and 150 for biotypes P2, P3 and P4, respectively. DNA sequencing and allele-specific PCR revealed that both biotypes P2 and P3 exhibit a single non-synonymous point mutation from TGG to TGC that leads to a well known Trp-2027-Cys mutation. Interestingly, the highly resistant biotype, P4, did not contain any of the known mutation except the newly discovered target point Asn-2097-Asp, which resulted from a nucleotide change in the codon AAT to GAT. ACCase gene expression was found differentially regulated in the susceptible biotype (P1) and highly resistant biotype P4 from 24 to 72h after treatment (HAT) when being treated with the recommended field rate (198gha-1) of fluazifop. However, the small and erratic differences of ACCase gene expression between biotype P1 and P4 does not support the 150-fold resistance in biotype P4. Therefore, the involvement of the target point Asn-2097-Asp and other non-target-site-based resistance mechanisms in the biotype P4 could not be ruled out.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalPesticide Biochemistry and Physiology
Volume111
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Eleusine
fluazifop
Eleusine indica
Malaysia
biotypes
Gene Expression
Acetyl-CoA Carboxylase
Mutation
R Factors
Herbicides
DNA Sequence Analysis
Point Mutation
Codon
Nucleotides
Alleles
Polymerase Chain Reaction
Population
mutation
gene expression
acetyl-CoA carboxylase

Keywords

  • ACCase gene expression
  • Goosegrass
  • Herbicide resistance
  • Mutation

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

Cite this

Molecular basis for resistance to ACCase-inhibiting fluazifop in Eleusine indica from Malaysia. / Cha, Thye San; Najihah, Mohamed Ghazani; Sahid, Ismail; Chuah, Tse Seng.

In: Pesticide Biochemistry and Physiology, Vol. 111, No. 1, 2014, p. 7-13.

Research output: Contribution to journalArticle

Cha, Thye San ; Najihah, Mohamed Ghazani ; Sahid, Ismail ; Chuah, Tse Seng. / Molecular basis for resistance to ACCase-inhibiting fluazifop in Eleusine indica from Malaysia. In: Pesticide Biochemistry and Physiology. 2014 ; Vol. 111, No. 1. pp. 7-13.
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