Molecular archeology of ancient bone from 400 year old shipwreck

Shahrul Hisham Zainal Ariffn, Rohaya Megat Abdul Wahab, Zulkefie Zamrod, Samsol Sahar, Mohd Fauzi Abd Razak, Eni Juliana Ariffn, Sahidan Senaf

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Molecular archeology is a new frontier of ancient archeology. Studies involving molecular archeology can give extra information useful to interpret our past. Ancient DNA basically consists of small DNA fragments that survive for a very long period. This DNA can be used as a template to study molecular archeology. We were able to isolate ancient mitochondrial DNA (mtDNA) from bones that were recovered by the Department of Museum and Antiquity Malaysia during their Wanli's shipwreck excavation project recently. The amount of recovered mtDNA was very small; extracted bone showed there was no DNA band after agarose electrophoresis. Amplifcation using PCR approach at D-loop I of human mitochondrial DNA was able to produce the amplifed DNA of 239bp in size. However, only 215 nucleic acids sequences were able to be confrmed when subjected to DNA sequencing procedures. Further analysis of the DNA sequenced of this amplifed DNA showed that 99% of the generated sequenced was identical with D-loop region I of human mitochondrial DNA. The results showed that our procedure of DNA extraction although produces very low amount of DNA but yields pure DNA that can be used for further analysis such as PCR amplifcation and DNA sequencing.

Original languageEnglish
Pages (from-to)27-31
Number of pages5
JournalAsia-Pacific Journal of Molecular Biology and Biotechnology
Volume15
Issue number1
Publication statusPublished - 2007

Fingerprint

Archaeology
Bone and Bones
DNA
Mitochondrial DNA
DNA Sequence Analysis
Polymerase Chain Reaction
Museums
Malaysia
Sepharose
Nucleic Acids
Electrophoresis

Keywords

  • Ancient mitochondrial DNA
  • Human bones
  • PCR amplifcation

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

Cite this

Ariffn, S. H. Z., Megat Abdul Wahab, R., Zamrod, Z., Sahar, S., Razak, M. F. A., Ariffn, E. J., & Senaf, S. (2007). Molecular archeology of ancient bone from 400 year old shipwreck. Asia-Pacific Journal of Molecular Biology and Biotechnology, 15(1), 27-31.

Molecular archeology of ancient bone from 400 year old shipwreck. / Ariffn, Shahrul Hisham Zainal; Megat Abdul Wahab, Rohaya; Zamrod, Zulkefie; Sahar, Samsol; Razak, Mohd Fauzi Abd; Ariffn, Eni Juliana; Senaf, Sahidan.

In: Asia-Pacific Journal of Molecular Biology and Biotechnology, Vol. 15, No. 1, 2007, p. 27-31.

Research output: Contribution to journalArticle

Ariffn, SHZ, Megat Abdul Wahab, R, Zamrod, Z, Sahar, S, Razak, MFA, Ariffn, EJ & Senaf, S 2007, 'Molecular archeology of ancient bone from 400 year old shipwreck', Asia-Pacific Journal of Molecular Biology and Biotechnology, vol. 15, no. 1, pp. 27-31.
Ariffn, Shahrul Hisham Zainal ; Megat Abdul Wahab, Rohaya ; Zamrod, Zulkefie ; Sahar, Samsol ; Razak, Mohd Fauzi Abd ; Ariffn, Eni Juliana ; Senaf, Sahidan. / Molecular archeology of ancient bone from 400 year old shipwreck. In: Asia-Pacific Journal of Molecular Biology and Biotechnology. 2007 ; Vol. 15, No. 1. pp. 27-31.
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