Abstract
In order to meet the increasing demand of oleochemical industries, genetic engineering was initiated to modify the fatty acid composition of oil palm with the aim to increase the production of oleic acid to be used as a feedstock. Biolistic approach was used for inserting an antisense palmitoyl-ACP- thioesterase sequence into oil palm tissues to down regulate the activity of palmitoyl-ACP-thioesterase, and increase the accumulation of oleic acid at the expense of palmitic acid. Genomic DNA was isolated from transgenic oil palm and polymerase chain reaction (PCR) amplification of the, selectable marker gene (bar) and gene of interest, antisense palmitoyl-ACP-thioesterase, was successfully carried out. The presence of transgenes was confirmed by DNA sequencing.
| Original language | English |
|---|---|
| Pages (from-to) | 96-103 |
| Number of pages | 8 |
| Journal | Journal of Oil Palm Research |
| Volume | 26 |
| Issue number | MAR |
| Publication status | Published - 2014 |
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Keywords
- Antisense PAT
- Bar gene
- PCR amplification
- Transgenic oil palm
ASJC Scopus subject areas
- Agronomy and Crop Science
- Food Science
- Renewable Energy, Sustainability and the Environment
- Biomaterials
Cite this
Molecular analysis of transgenic oil palm to detect the presence of transgenes. / Nurfahisza, A. Rahman; Rafiqah, Md Aman; Masani, Mat Yunus Abdul; Hanin, Ayub Nor; Rasid, Omar Abdul; Parveez, Ghulam Kadir Ahmad; Ismail, Ismanizan.
In: Journal of Oil Palm Research, Vol. 26, No. MAR, 2014, p. 96-103.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular analysis of transgenic oil palm to detect the presence of transgenes
AU - Nurfahisza, A. Rahman
AU - Rafiqah, Md Aman
AU - Masani, Mat Yunus Abdul
AU - Hanin, Ayub Nor
AU - Rasid, Omar Abdul
AU - Parveez, Ghulam Kadir Ahmad
AU - Ismail, Ismanizan
PY - 2014
Y1 - 2014
N2 - In order to meet the increasing demand of oleochemical industries, genetic engineering was initiated to modify the fatty acid composition of oil palm with the aim to increase the production of oleic acid to be used as a feedstock. Biolistic approach was used for inserting an antisense palmitoyl-ACP- thioesterase sequence into oil palm tissues to down regulate the activity of palmitoyl-ACP-thioesterase, and increase the accumulation of oleic acid at the expense of palmitic acid. Genomic DNA was isolated from transgenic oil palm and polymerase chain reaction (PCR) amplification of the, selectable marker gene (bar) and gene of interest, antisense palmitoyl-ACP-thioesterase, was successfully carried out. The presence of transgenes was confirmed by DNA sequencing.
AB - In order to meet the increasing demand of oleochemical industries, genetic engineering was initiated to modify the fatty acid composition of oil palm with the aim to increase the production of oleic acid to be used as a feedstock. Biolistic approach was used for inserting an antisense palmitoyl-ACP- thioesterase sequence into oil palm tissues to down regulate the activity of palmitoyl-ACP-thioesterase, and increase the accumulation of oleic acid at the expense of palmitic acid. Genomic DNA was isolated from transgenic oil palm and polymerase chain reaction (PCR) amplification of the, selectable marker gene (bar) and gene of interest, antisense palmitoyl-ACP-thioesterase, was successfully carried out. The presence of transgenes was confirmed by DNA sequencing.
KW - Antisense PAT
KW - Bar gene
KW - PCR amplification
KW - Transgenic oil palm
UR - http://www.scopus.com/inward/record.url?scp=84897051468&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84897051468&partnerID=8YFLogxK
M3 - Article
VL - 26
SP - 96
EP - 103
JO - Journal of Oil Palm Research
JF - Journal of Oil Palm Research
SN - 1511-2780
IS - MAR
ER -