Molecular analysis of transgenic oil palm to detect the presence of transgenes

A. Rahman Nurfahisza, Md Aman Rafiqah, Mat Yunus Abdul Masani, Ayub Nor Hanin, Omar Abdul Rasid, Ghulam Kadir Ahmad Parveez, Ismanizan Ismail

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In order to meet the increasing demand of oleochemical industries, genetic engineering was initiated to modify the fatty acid composition of oil palm with the aim to increase the production of oleic acid to be used as a feedstock. Biolistic approach was used for inserting an antisense palmitoyl-ACP- thioesterase sequence into oil palm tissues to down regulate the activity of palmitoyl-ACP-thioesterase, and increase the accumulation of oleic acid at the expense of palmitic acid. Genomic DNA was isolated from transgenic oil palm and polymerase chain reaction (PCR) amplification of the, selectable marker gene (bar) and gene of interest, antisense palmitoyl-ACP-thioesterase, was successfully carried out. The presence of transgenes was confirmed by DNA sequencing.

Original languageEnglish
Pages (from-to)96-103
Number of pages8
JournalJournal of Oil Palm Research
Volume26
Issue numberMAR
Publication statusPublished - 2014

Fingerprint

Palm oil
Elaeis guineensis
Transgenes
transgenes
Oils
Oleic acid
genetically modified organisms
Oleic Acid
oleic acid
DNA
Genes
Biolistics
Palmitic acid
Genetic engineering
biolistics
Genetic Engineering
Palmitic Acid
Polymerase chain reaction
feedstocks
DNA Sequence Analysis

Keywords

  • Antisense PAT
  • Bar gene
  • PCR amplification
  • Transgenic oil palm

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Renewable Energy, Sustainability and the Environment
  • Biomaterials

Cite this

Nurfahisza, A. R., Rafiqah, M. A., Masani, M. Y. A., Hanin, A. N., Rasid, O. A., Parveez, G. K. A., & Ismail, I. (2014). Molecular analysis of transgenic oil palm to detect the presence of transgenes. Journal of Oil Palm Research, 26(MAR), 96-103.

Molecular analysis of transgenic oil palm to detect the presence of transgenes. / Nurfahisza, A. Rahman; Rafiqah, Md Aman; Masani, Mat Yunus Abdul; Hanin, Ayub Nor; Rasid, Omar Abdul; Parveez, Ghulam Kadir Ahmad; Ismail, Ismanizan.

In: Journal of Oil Palm Research, Vol. 26, No. MAR, 2014, p. 96-103.

Research output: Contribution to journalArticle

Nurfahisza, AR, Rafiqah, MA, Masani, MYA, Hanin, AN, Rasid, OA, Parveez, GKA & Ismail, I 2014, 'Molecular analysis of transgenic oil palm to detect the presence of transgenes', Journal of Oil Palm Research, vol. 26, no. MAR, pp. 96-103.
Nurfahisza AR, Rafiqah MA, Masani MYA, Hanin AN, Rasid OA, Parveez GKA et al. Molecular analysis of transgenic oil palm to detect the presence of transgenes. Journal of Oil Palm Research. 2014;26(MAR):96-103.
Nurfahisza, A. Rahman ; Rafiqah, Md Aman ; Masani, Mat Yunus Abdul ; Hanin, Ayub Nor ; Rasid, Omar Abdul ; Parveez, Ghulam Kadir Ahmad ; Ismail, Ismanizan. / Molecular analysis of transgenic oil palm to detect the presence of transgenes. In: Journal of Oil Palm Research. 2014 ; Vol. 26, No. MAR. pp. 96-103.
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