Modulatory effect of phytoestrogens and curcumin on induction of annexin 1 in human peripheral blood mononuclear cells and their inhibitory effect on secretory phospholipase A2

Waqas Ahmad, Kumolosasi Msi Endang, Ibrahim Jantan, Malina Jasamai, Emil Salim

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4 Citations (Scopus)

Abstract

Purpose: To investigate the modulatory effects of phytoestrogens (coumestrol, daidzein and genistein) and curcumin on the induction and secretion of annexin-1 (ANXA-1) in human peripheral blood mononuclear cells (PBMCs) under inflammatory and non-inflammatory conditions, as well as their effect on the activity of phospholipase A2-V (sPLA2-V). Methods: The modulatory effects of phytoestrogens and curcumin on the induction ofANXA1 were investigated via sandwich ELISA method, while their effects on the activity of sPLA2-V were determined by photometric assays. Besides, the cell viability of these compounds was determined by standard trypan blue exclusion method using PBMCs. Results: The results indicate a significant increase (p < 0.05) in the total content ofANXA1, particularly by coumestrol (p < 0.01), in both inflammatory and non-inflammatory cells. Besides, the compounds also exhibited a dose-dependent inhibition of sPLA2-V activity; however, among these compounds, curcumin and genistein were the strongest inhibitors with an IC50 value of 11.1 ± 0.3 μM and13.6 ± 0.6 μM respectively. Conclusion: The investigated compounds have a potential to induce synthesis and secretion ofANXA1 as well as inhibitory activity of sPLA2-V, suggesting their inhibitory role in phospholipid metabolism and inflammation.

Original languageEnglish
Pages (from-to)171-177
Number of pages7
JournalTropical Journal of Pharmaceutical Research
Volume13
Issue number2
DOIs
Publication statusPublished - Feb 2014

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Secretory Phospholipase A2
Annexins
Phytoestrogens
Curcumin
Coumestrol
Blood Cells
Genistein
Trypan Blue
Phospholipases A2
Inhibitory Concentration 50
Phospholipids
Cell Survival
Enzyme-Linked Immunosorbent Assay
Inflammation

Keywords

  • Annexin-1
  • Curcumin
  • Inflammation
  • Phospholipase A
  • Phytoestrogens

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology (medical)

Cite this

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title = "Modulatory effect of phytoestrogens and curcumin on induction of annexin 1 in human peripheral blood mononuclear cells and their inhibitory effect on secretory phospholipase A2",
abstract = "Purpose: To investigate the modulatory effects of phytoestrogens (coumestrol, daidzein and genistein) and curcumin on the induction and secretion of annexin-1 (ANXA-1) in human peripheral blood mononuclear cells (PBMCs) under inflammatory and non-inflammatory conditions, as well as their effect on the activity of phospholipase A2-V (sPLA2-V). Methods: The modulatory effects of phytoestrogens and curcumin on the induction ofANXA1 were investigated via sandwich ELISA method, while their effects on the activity of sPLA2-V were determined by photometric assays. Besides, the cell viability of these compounds was determined by standard trypan blue exclusion method using PBMCs. Results: The results indicate a significant increase (p < 0.05) in the total content ofANXA1, particularly by coumestrol (p < 0.01), in both inflammatory and non-inflammatory cells. Besides, the compounds also exhibited a dose-dependent inhibition of sPLA2-V activity; however, among these compounds, curcumin and genistein were the strongest inhibitors with an IC50 value of 11.1 ± 0.3 μM and13.6 ± 0.6 μM respectively. Conclusion: The investigated compounds have a potential to induce synthesis and secretion ofANXA1 as well as inhibitory activity of sPLA2-V, suggesting their inhibitory role in phospholipid metabolism and inflammation.",
keywords = "Annexin-1, Curcumin, Inflammation, Phospholipase A, Phytoestrogens",
author = "Waqas Ahmad and Endang, {Kumolosasi Msi} and Ibrahim Jantan and Malina Jasamai and Emil Salim",
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T1 - Modulatory effect of phytoestrogens and curcumin on induction of annexin 1 in human peripheral blood mononuclear cells and their inhibitory effect on secretory phospholipase A2

AU - Ahmad, Waqas

AU - Endang, Kumolosasi Msi

AU - Jantan, Ibrahim

AU - Jasamai, Malina

AU - Salim, Emil

PY - 2014/2

Y1 - 2014/2

N2 - Purpose: To investigate the modulatory effects of phytoestrogens (coumestrol, daidzein and genistein) and curcumin on the induction and secretion of annexin-1 (ANXA-1) in human peripheral blood mononuclear cells (PBMCs) under inflammatory and non-inflammatory conditions, as well as their effect on the activity of phospholipase A2-V (sPLA2-V). Methods: The modulatory effects of phytoestrogens and curcumin on the induction ofANXA1 were investigated via sandwich ELISA method, while their effects on the activity of sPLA2-V were determined by photometric assays. Besides, the cell viability of these compounds was determined by standard trypan blue exclusion method using PBMCs. Results: The results indicate a significant increase (p < 0.05) in the total content ofANXA1, particularly by coumestrol (p < 0.01), in both inflammatory and non-inflammatory cells. Besides, the compounds also exhibited a dose-dependent inhibition of sPLA2-V activity; however, among these compounds, curcumin and genistein were the strongest inhibitors with an IC50 value of 11.1 ± 0.3 μM and13.6 ± 0.6 μM respectively. Conclusion: The investigated compounds have a potential to induce synthesis and secretion ofANXA1 as well as inhibitory activity of sPLA2-V, suggesting their inhibitory role in phospholipid metabolism and inflammation.

AB - Purpose: To investigate the modulatory effects of phytoestrogens (coumestrol, daidzein and genistein) and curcumin on the induction and secretion of annexin-1 (ANXA-1) in human peripheral blood mononuclear cells (PBMCs) under inflammatory and non-inflammatory conditions, as well as their effect on the activity of phospholipase A2-V (sPLA2-V). Methods: The modulatory effects of phytoestrogens and curcumin on the induction ofANXA1 were investigated via sandwich ELISA method, while their effects on the activity of sPLA2-V were determined by photometric assays. Besides, the cell viability of these compounds was determined by standard trypan blue exclusion method using PBMCs. Results: The results indicate a significant increase (p < 0.05) in the total content ofANXA1, particularly by coumestrol (p < 0.01), in both inflammatory and non-inflammatory cells. Besides, the compounds also exhibited a dose-dependent inhibition of sPLA2-V activity; however, among these compounds, curcumin and genistein were the strongest inhibitors with an IC50 value of 11.1 ± 0.3 μM and13.6 ± 0.6 μM respectively. Conclusion: The investigated compounds have a potential to induce synthesis and secretion ofANXA1 as well as inhibitory activity of sPLA2-V, suggesting their inhibitory role in phospholipid metabolism and inflammation.

KW - Annexin-1

KW - Curcumin

KW - Inflammation

KW - Phospholipase A

KW - Phytoestrogens

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