Modulation of Proteome Profile in AβPP/PS1 Mice Hippocampus, Medial Prefrontal Cortex, and Striatum by Palm Oil Derived Tocotrienol-Rich Fraction

Hamizah Shahirah Hamezah, Lina Wati Durani, Daijiro Yanagisawa, Nor Faeizah Ibrahim, Wan Mohd Aizat, Suzana Makpol, Wan Zurinah Wan Ngah, Hanafi Ahmad Damanhuri, Ikuo Tooyama

Research output: Contribution to journalArticle

Abstract

Tocotrienol-rich fraction (TRF) is a mixture of vitamin E analogs derived from palm oil. We previously demonstrated that supplementation with TRF improved cognitive function and modulated amyloid pathology in AβPP/PS1 mice brains. The current study was designed to examine proteomic profiles underlying the therapeutic effect of TRF in the brain. Proteomic analyses were performed on samples of hippocampus, medial prefrontal cortex (mPFC), and striatum using liquid chromatography coupled to Q Exactive HF Orbitrap mass spectrometry. From these analyses, we profiled a total of 5,847 proteins of which 155 proteins were differentially expressed between AβPP/PS1 and wild-type mice. TRF supplementation of these mice altered the expression of 255 proteins in the hippocampus, mPFC, and striatum. TRF also negatively modulated the expression of amyloid beta A4 protein and receptor-type tyrosine-protein phosphatase alpha protein in the hippocampus. The expression of proteins in metabolic pathways, oxidative phosphorylation, and those involved in Alzheimer's disease were altered in the brains of AβPP/PS1 mice that received TRF supplementation.

Original languageEnglish
Pages (from-to)229-246
Number of pages18
JournalJournal of Alzheimer's Disease
Volume72
Issue number1
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Tocotrienols
Proteome
Prefrontal Cortex
Hippocampus
Proteins
Proteomics
Class 4 Receptor-Like Protein Tyrosine Phosphatases
Brain
Amyloid beta-Peptides
Oxidative Phosphorylation
Therapeutic Uses
Metabolic Networks and Pathways
Vitamin E
Amyloid
Liquid Chromatography
Cognition
palm oil
Mass Spectrometry
Alzheimer Disease
Pathology

Keywords

  • Alzheimer's disease
  • LC-MS
  • palm oil
  • proteomics
  • tocotrienol

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Psychology
  • Geriatrics and Gerontology
  • Psychiatry and Mental health

Cite this

Modulation of Proteome Profile in AβPP/PS1 Mice Hippocampus, Medial Prefrontal Cortex, and Striatum by Palm Oil Derived Tocotrienol-Rich Fraction. / Hamezah, Hamizah Shahirah; Durani, Lina Wati; Yanagisawa, Daijiro; Ibrahim, Nor Faeizah; Aizat, Wan Mohd; Makpol, Suzana; Wan Ngah, Wan Zurinah; Damanhuri, Hanafi Ahmad; Tooyama, Ikuo.

In: Journal of Alzheimer's Disease, Vol. 72, No. 1, 01.01.2019, p. 229-246.

Research output: Contribution to journalArticle

Hamezah, Hamizah Shahirah ; Durani, Lina Wati ; Yanagisawa, Daijiro ; Ibrahim, Nor Faeizah ; Aizat, Wan Mohd ; Makpol, Suzana ; Wan Ngah, Wan Zurinah ; Damanhuri, Hanafi Ahmad ; Tooyama, Ikuo. / Modulation of Proteome Profile in AβPP/PS1 Mice Hippocampus, Medial Prefrontal Cortex, and Striatum by Palm Oil Derived Tocotrienol-Rich Fraction. In: Journal of Alzheimer's Disease. 2019 ; Vol. 72, No. 1. pp. 229-246.
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