Modelling the kinetics of hexavalent molybdenum (Mo6+) reduction by the Serratia sp. strain MIE2 in batch culture

Mohd Izuan Effendi Halmi, Siti Rozaimah Sheikh Abdullah, Wan Lutfi Wan Johari, Mohd Shukuri Muhammad Ali, Noor Azmi Shaharuddin, Ariff Khalid, Mohd Yunus Shukor

Research output: Contribution to journalArticle

Abstract

In the present work, the kinetics of hexavalent molybdenum reduction by the Serratia sp. strain MIE2 were investigated using several kinetic models, such as Monod, Haldane, Teissier, Aiba, Yano, Han and Levenspiel and Luong. The statistical analysis showed that the best model was Teissier, which had the lowest RMSE and AICc values, the highest adjusted R2 values, and an F test and with a bias factor and an accuracy factor nearest to unity (1.0). The calculated value for the Teissier constants, such as pmax, Ks and Ki, was 0.506 µmol Mo-blue h−1, 6.53 mM and 29.41 mM, respectively. The effect of heavy metals showed that hexavalent molybdenum reduction by the strain MIE2 was inhibited by silver, mercury and copper with a total inhibition of 96, 97, and 45 %, respectively, at a concentration of 1 ppm. Otherwise, the Mo-reducing enzyme was inhibited by mercury and zinc with an inhibition of 88 and 65 %, respectively. Most of the respiratory inhibitors did not inhibit the Mo-reducing enzyme activity, indicating that the respiratory system in this bacterium is not the site of the hexavalent molybdenum reduction. The results obtained from this study could be useful for estimating the relationship between molybdenum-blue production and the molybdate concentration, which may be important during the up scaling of the molybdenum bioremediation process.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalATTI Della Accademia Nazionale Dei Lincei Rendiconti Lincei Scienze Fisiche E Naturali
DOIs
Publication statusAccepted/In press - 4 Jul 2016

Fingerprint

Serratia
Batch Cell Culture Techniques
Molybdenum
molybdenum
kinetics
Mercury
modeling
mercury
Environmental Biodegradation
Enzymes
molybdates
Heavy Metals
Silver
Respiratory System
bioremediation
Zinc
Copper
respiratory system
silver
enzyme activity

Keywords

  • Bioremediation
  • Heavy metals
  • Modelling kinetics
  • Molybdenum
  • Respiratory inhibitors

ASJC Scopus subject areas

  • Earth and Planetary Sciences(all)
  • Agricultural and Biological Sciences(all)
  • Environmental Science(all)

Cite this

Modelling the kinetics of hexavalent molybdenum (Mo6+) reduction by the Serratia sp. strain MIE2 in batch culture. / Halmi, Mohd Izuan Effendi; Sheikh Abdullah, Siti Rozaimah; Johari, Wan Lutfi Wan; Ali, Mohd Shukuri Muhammad; Shaharuddin, Noor Azmi; Khalid, Ariff; Shukor, Mohd Yunus.

In: ATTI Della Accademia Nazionale Dei Lincei Rendiconti Lincei Scienze Fisiche E Naturali, 04.07.2016, p. 1-11.

Research output: Contribution to journalArticle

Halmi, Mohd Izuan Effendi ; Sheikh Abdullah, Siti Rozaimah ; Johari, Wan Lutfi Wan ; Ali, Mohd Shukuri Muhammad ; Shaharuddin, Noor Azmi ; Khalid, Ariff ; Shukor, Mohd Yunus. / Modelling the kinetics of hexavalent molybdenum (Mo6+) reduction by the Serratia sp. strain MIE2 in batch culture. In: ATTI Della Accademia Nazionale Dei Lincei Rendiconti Lincei Scienze Fisiche E Naturali. 2016 ; pp. 1-11.
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AU - Sheikh Abdullah, Siti Rozaimah

AU - Johari, Wan Lutfi Wan

AU - Ali, Mohd Shukuri Muhammad

AU - Shaharuddin, Noor Azmi

AU - Khalid, Ariff

AU - Shukor, Mohd Yunus

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N2 - In the present work, the kinetics of hexavalent molybdenum reduction by the Serratia sp. strain MIE2 were investigated using several kinetic models, such as Monod, Haldane, Teissier, Aiba, Yano, Han and Levenspiel and Luong. The statistical analysis showed that the best model was Teissier, which had the lowest RMSE and AICc values, the highest adjusted R2 values, and an F test and with a bias factor and an accuracy factor nearest to unity (1.0). The calculated value for the Teissier constants, such as pmax, Ks and Ki, was 0.506 µmol Mo-blue h−1, 6.53 mM and 29.41 mM, respectively. The effect of heavy metals showed that hexavalent molybdenum reduction by the strain MIE2 was inhibited by silver, mercury and copper with a total inhibition of 96, 97, and 45 %, respectively, at a concentration of 1 ppm. Otherwise, the Mo-reducing enzyme was inhibited by mercury and zinc with an inhibition of 88 and 65 %, respectively. Most of the respiratory inhibitors did not inhibit the Mo-reducing enzyme activity, indicating that the respiratory system in this bacterium is not the site of the hexavalent molybdenum reduction. The results obtained from this study could be useful for estimating the relationship between molybdenum-blue production and the molybdate concentration, which may be important during the up scaling of the molybdenum bioremediation process.

AB - In the present work, the kinetics of hexavalent molybdenum reduction by the Serratia sp. strain MIE2 were investigated using several kinetic models, such as Monod, Haldane, Teissier, Aiba, Yano, Han and Levenspiel and Luong. The statistical analysis showed that the best model was Teissier, which had the lowest RMSE and AICc values, the highest adjusted R2 values, and an F test and with a bias factor and an accuracy factor nearest to unity (1.0). The calculated value for the Teissier constants, such as pmax, Ks and Ki, was 0.506 µmol Mo-blue h−1, 6.53 mM and 29.41 mM, respectively. The effect of heavy metals showed that hexavalent molybdenum reduction by the strain MIE2 was inhibited by silver, mercury and copper with a total inhibition of 96, 97, and 45 %, respectively, at a concentration of 1 ppm. Otherwise, the Mo-reducing enzyme was inhibited by mercury and zinc with an inhibition of 88 and 65 %, respectively. Most of the respiratory inhibitors did not inhibit the Mo-reducing enzyme activity, indicating that the respiratory system in this bacterium is not the site of the hexavalent molybdenum reduction. The results obtained from this study could be useful for estimating the relationship between molybdenum-blue production and the molybdate concentration, which may be important during the up scaling of the molybdenum bioremediation process.

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