Modeling microfluidic DNA extraction using superparamagnetic bead particles in COMSOL multiphysics simulation

Gauri Samla, Gan Kok Beng, Sue Mian Then

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

High throughput DNA extraction method by superparamagnetic beads particles in microfluidic platform was studied using COMSOL multiphysics simulation software. The separation of DNA is based on solid phase microextraction method to isolate, fractionate and concentrate the analyte from complex sample matrices as well as implemented on microfluidic device with minimal procedures. The conceptual of the efficient flow design of microfluidic channel and the separation of DNA with superparamagnetic beads has shown in the COMSOL multiphysics simulation with complete calculation for every study steps. The purity of extracted DNA yield also has presented in the simulation and proved the sample can be used for further downstream process such as polymerase chain reaction analysis. Based on the simulation, 83.26 % of DNA yield able to be extracted using superparamagnetic beads and the rest of molecules are beads with absorbed DNA onto it. Even so, further DNA process typically, polymerase chain reaction possibly efficacious in the presence of superparamagnetic beads due to the addition of elution buffers in the following analysis.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalMicrosystem Technologies
DOIs
Publication statusAccepted/In press - 22 Oct 2016

Fingerprint

Microfluidics
beads
DNA
deoxyribonucleic acid
simulation
polymerase chain reaction
Polymerase chain reaction
elution
microfluidic devices
solid phases
Buffers
purity
platforms
buffers
Throughput
computer programs
Molecules
matrices
molecules

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Modeling microfluidic DNA extraction using superparamagnetic bead particles in COMSOL multiphysics simulation. / Samla, Gauri; Kok Beng, Gan; Then, Sue Mian.

In: Microsystem Technologies, 22.10.2016, p. 1-6.

Research output: Contribution to journalArticle

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