Mode of action of endophytic streptomyces sp., SUK 25 extracts against MRSA; microscopic, Biochemical and time-kill analysis

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Abstract

Bioactive compound from endophytic Streptomyces sp. has been claimed as a source of antibiotic. This study focused on the investigation of pharmacodynamic pattern and visualization of the mechanism of SUK 25 extracts against MRSA. The pharmacodynamic characteristic of the extract against MRSA 43300 was determined using time-kill assay. Then, the mode of action of the extracts was observed through biochemical assay and transmission electron microscopy. The SUK 25 extracts displayed bacteriostatic mode of action and concentration-dependent manner. The action of SUK 25 extracts against MRSA ATCC 43300 caused irregular shape of cells, which affected changes in Crystal Violet uptake by the cells. The release of UV absorbing materials and protein from the cells was caused by cell lysis. In conclusion, the action of the SUK 25 extracts against MRSA ATCC 43300 led to the internal change in cells, through which permeability of cells was altered by a decrease in the Crystal Violet uptake, shape of cell changes, and in turn brought about the lysis of cell.

Original languageEnglish
Article number03
Pages (from-to)11-17
Number of pages7
JournalInternational Journal of Pharmaceutical Sciences Review and Research
Volume30
Issue number1
Publication statusPublished - 1 Jan 2015

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Streptomyces
Methicillin-Resistant Staphylococcus aureus
Gentian Violet
Cell Shape
Transmission Electron Microscopy
Permeability
Anti-Bacterial Agents
Proteins

Keywords

  • Endophytic streptomyces
  • Mode of action
  • MRSA

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

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abstract = "Bioactive compound from endophytic Streptomyces sp. has been claimed as a source of antibiotic. This study focused on the investigation of pharmacodynamic pattern and visualization of the mechanism of SUK 25 extracts against MRSA. The pharmacodynamic characteristic of the extract against MRSA 43300 was determined using time-kill assay. Then, the mode of action of the extracts was observed through biochemical assay and transmission electron microscopy. The SUK 25 extracts displayed bacteriostatic mode of action and concentration-dependent manner. The action of SUK 25 extracts against MRSA ATCC 43300 caused irregular shape of cells, which affected changes in Crystal Violet uptake by the cells. The release of UV absorbing materials and protein from the cells was caused by cell lysis. In conclusion, the action of the SUK 25 extracts against MRSA ATCC 43300 led to the internal change in cells, through which permeability of cells was altered by a decrease in the Crystal Violet uptake, shape of cell changes, and in turn brought about the lysis of cell.",
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AU - Ahmad, Siti Junaidah

AU - Hing, Hiang Lian

AU - Basri, Dayang Fredalina

AU - Mohamad Zin, Noraziah

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