Mitragyna speciosa-induced hepatotoxicity-treated effectively by piper betle: Scope as a future antidote

Haszianaliza Haslan, Farihah Suhaimi, Srijit Das

Research output: Contribution to journalArticle

Abstract

Objective: Consumption of Mitragyna speciosa (MS) leads to various toxicities including hepatotoxicity. Piper betle (PB) is a herb that possesses various therapeutic properties. The aim of the present study was to examine the protective effect of PB methanol extract (PBME) on MS-induced hepatotoxicity which could pave the way for any future antidote. Methods: Twenty-four male Sprague–Dawley rats were randomized into control and experimental groups. The control group was further divided into the negative (G-T80) and positive (G-PB) control groups. The G-T80 group (n=6) received oral gavage of the vehicle, 15% Tween 80. The G-PB group (n=6) received PBME 200 mg/kg/day, orally. The experimental group was divided into two groups, i.e., The G-MS and G-MS (PB) groups. The G-MS group (n=6) received only MS methanol extract (MSME) 500 mg/kg/day, while the G-MS (PB) group (n=6) received MSME with concomitant treatment with PBME. Results: Histopathology examination of the G-T80 and G-PB groups showed normal histology of the liver. The G-MS group showed liver injury features such as microvesicular steatosis, ballooning degeneration, acidophilic bodies, scattered focal necrosis, fibrous portal expansion, bridging fibrosis, sinusoidal congestion, and dilatation. These features were fewer in the G-MS (PB) group which received concomitant treatment with PBME. Conclusion: Administration of PBME exerted a protective effect against MS-induced hepatotoxicity. Future clinical trials using PB as an antidote may help in combating MS-induced hepatoxicity.

Original languageEnglish
Pages (from-to)43-46
Number of pages4
JournalAsian Journal of Pharmaceutical and Clinical Research
Volume11
Issue number3
DOIs
Publication statusPublished - 1 Mar 2018

Fingerprint

Mitragyna
Piper betle
Antidotes
Methanol
Control Groups
Polysorbates
Liver

Keywords

  • Fibrosis
  • Liver injury
  • Methanol extract
  • Microvesicular steatosis
  • Mitragyna speciosa
  • Piper betle

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Pharmacology (medical)

Cite this

Mitragyna speciosa-induced hepatotoxicity-treated effectively by piper betle : Scope as a future antidote. / Haslan, Haszianaliza; Suhaimi, Farihah; Das, Srijit.

In: Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, No. 3, 01.03.2018, p. 43-46.

Research output: Contribution to journalArticle

@article{616b8a3e98204d2aa30c0d88536a5fce,
title = "Mitragyna speciosa-induced hepatotoxicity-treated effectively by piper betle: Scope as a future antidote",
abstract = "Objective: Consumption of Mitragyna speciosa (MS) leads to various toxicities including hepatotoxicity. Piper betle (PB) is a herb that possesses various therapeutic properties. The aim of the present study was to examine the protective effect of PB methanol extract (PBME) on MS-induced hepatotoxicity which could pave the way for any future antidote. Methods: Twenty-four male Sprague–Dawley rats were randomized into control and experimental groups. The control group was further divided into the negative (G-T80) and positive (G-PB) control groups. The G-T80 group (n=6) received oral gavage of the vehicle, 15{\%} Tween 80. The G-PB group (n=6) received PBME 200 mg/kg/day, orally. The experimental group was divided into two groups, i.e., The G-MS and G-MS (PB) groups. The G-MS group (n=6) received only MS methanol extract (MSME) 500 mg/kg/day, while the G-MS (PB) group (n=6) received MSME with concomitant treatment with PBME. Results: Histopathology examination of the G-T80 and G-PB groups showed normal histology of the liver. The G-MS group showed liver injury features such as microvesicular steatosis, ballooning degeneration, acidophilic bodies, scattered focal necrosis, fibrous portal expansion, bridging fibrosis, sinusoidal congestion, and dilatation. These features were fewer in the G-MS (PB) group which received concomitant treatment with PBME. Conclusion: Administration of PBME exerted a protective effect against MS-induced hepatotoxicity. Future clinical trials using PB as an antidote may help in combating MS-induced hepatoxicity.",
keywords = "Fibrosis, Liver injury, Methanol extract, Microvesicular steatosis, Mitragyna speciosa, Piper betle",
author = "Haszianaliza Haslan and Farihah Suhaimi and Srijit Das",
year = "2018",
month = "3",
day = "1",
doi = "10.22159/ajpcr.2018.v11i3.22958",
language = "English",
volume = "11",
pages = "43--46",
journal = "Asian Journal of Pharmaceutical and Clinical Research",
issn = "0974-2441",
publisher = "Asian Journal of Pharmaceutical and Clinical Research",
number = "3",

}

TY - JOUR

T1 - Mitragyna speciosa-induced hepatotoxicity-treated effectively by piper betle

T2 - Scope as a future antidote

AU - Haslan, Haszianaliza

AU - Suhaimi, Farihah

AU - Das, Srijit

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Objective: Consumption of Mitragyna speciosa (MS) leads to various toxicities including hepatotoxicity. Piper betle (PB) is a herb that possesses various therapeutic properties. The aim of the present study was to examine the protective effect of PB methanol extract (PBME) on MS-induced hepatotoxicity which could pave the way for any future antidote. Methods: Twenty-four male Sprague–Dawley rats were randomized into control and experimental groups. The control group was further divided into the negative (G-T80) and positive (G-PB) control groups. The G-T80 group (n=6) received oral gavage of the vehicle, 15% Tween 80. The G-PB group (n=6) received PBME 200 mg/kg/day, orally. The experimental group was divided into two groups, i.e., The G-MS and G-MS (PB) groups. The G-MS group (n=6) received only MS methanol extract (MSME) 500 mg/kg/day, while the G-MS (PB) group (n=6) received MSME with concomitant treatment with PBME. Results: Histopathology examination of the G-T80 and G-PB groups showed normal histology of the liver. The G-MS group showed liver injury features such as microvesicular steatosis, ballooning degeneration, acidophilic bodies, scattered focal necrosis, fibrous portal expansion, bridging fibrosis, sinusoidal congestion, and dilatation. These features were fewer in the G-MS (PB) group which received concomitant treatment with PBME. Conclusion: Administration of PBME exerted a protective effect against MS-induced hepatotoxicity. Future clinical trials using PB as an antidote may help in combating MS-induced hepatoxicity.

AB - Objective: Consumption of Mitragyna speciosa (MS) leads to various toxicities including hepatotoxicity. Piper betle (PB) is a herb that possesses various therapeutic properties. The aim of the present study was to examine the protective effect of PB methanol extract (PBME) on MS-induced hepatotoxicity which could pave the way for any future antidote. Methods: Twenty-four male Sprague–Dawley rats were randomized into control and experimental groups. The control group was further divided into the negative (G-T80) and positive (G-PB) control groups. The G-T80 group (n=6) received oral gavage of the vehicle, 15% Tween 80. The G-PB group (n=6) received PBME 200 mg/kg/day, orally. The experimental group was divided into two groups, i.e., The G-MS and G-MS (PB) groups. The G-MS group (n=6) received only MS methanol extract (MSME) 500 mg/kg/day, while the G-MS (PB) group (n=6) received MSME with concomitant treatment with PBME. Results: Histopathology examination of the G-T80 and G-PB groups showed normal histology of the liver. The G-MS group showed liver injury features such as microvesicular steatosis, ballooning degeneration, acidophilic bodies, scattered focal necrosis, fibrous portal expansion, bridging fibrosis, sinusoidal congestion, and dilatation. These features were fewer in the G-MS (PB) group which received concomitant treatment with PBME. Conclusion: Administration of PBME exerted a protective effect against MS-induced hepatotoxicity. Future clinical trials using PB as an antidote may help in combating MS-induced hepatoxicity.

KW - Fibrosis

KW - Liver injury

KW - Methanol extract

KW - Microvesicular steatosis

KW - Mitragyna speciosa

KW - Piper betle

UR - http://www.scopus.com/inward/record.url?scp=85042946107&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85042946107&partnerID=8YFLogxK

U2 - 10.22159/ajpcr.2018.v11i3.22958

DO - 10.22159/ajpcr.2018.v11i3.22958

M3 - Article

AN - SCOPUS:85042946107

VL - 11

SP - 43

EP - 46

JO - Asian Journal of Pharmaceutical and Clinical Research

JF - Asian Journal of Pharmaceutical and Clinical Research

SN - 0974-2441

IS - 3

ER -