miR-CATCH

MicroRNA capture affinity technology

Sebastian Vencken, Hassan Tidi Maharani, Noel G. McElvaney, Stephen G J Smith, Catherine M. Greene

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Several experimental methods exist to explore the microRNA (miRNA) regulome. These methods almost exclusively focus on multiple targets bound to a single, or perhaps a few miRNAs of interest. Here, we describe a microRNA capture affinity technology (miR-CATCH) which uses an affinity capture oligonucleotide to co-purify a single target messenger RNA (mRNA) together with all its endogenously bound miRNAs. This bench-top method is similar to RNA immunoprecipitation (RIP) and provides an experimental alternative to computational miRNA target prediction.

Original languageEnglish
Title of host publicationRNA Interference: Challenges and Therapeutic Opportunities
PublisherSpringer New York
Pages365-373
Number of pages9
ISBN (Electronic)9781493915385
ISBN (Print)9781493915378
DOIs
Publication statusPublished - 15 Oct 2014
Externally publishedYes

Fingerprint

MicroRNAs
Technology
Immunoprecipitation
Oligonucleotides
RNA
Messenger RNA

Keywords

  • Affinity capture
  • MicroRNA
  • MicroRNA target analysis
  • MiR-CATCH
  • Oligonucleotide hybridization

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Vencken, S., Tidi Maharani, H., McElvaney, N. G., Smith, S. G. J., & Greene, C. M. (2014). miR-CATCH: MicroRNA capture affinity technology. In RNA Interference: Challenges and Therapeutic Opportunities (pp. 365-373). Springer New York. https://doi.org/10.1007/978-1-4939-1538-5_23

miR-CATCH : MicroRNA capture affinity technology. / Vencken, Sebastian; Tidi Maharani, Hassan; McElvaney, Noel G.; Smith, Stephen G J; Greene, Catherine M.

RNA Interference: Challenges and Therapeutic Opportunities. Springer New York, 2014. p. 365-373.

Research output: Chapter in Book/Report/Conference proceedingChapter

Vencken, S, Tidi Maharani, H, McElvaney, NG, Smith, SGJ & Greene, CM 2014, miR-CATCH: MicroRNA capture affinity technology. in RNA Interference: Challenges and Therapeutic Opportunities. Springer New York, pp. 365-373. https://doi.org/10.1007/978-1-4939-1538-5_23
Vencken S, Tidi Maharani H, McElvaney NG, Smith SGJ, Greene CM. miR-CATCH: MicroRNA capture affinity technology. In RNA Interference: Challenges and Therapeutic Opportunities. Springer New York. 2014. p. 365-373 https://doi.org/10.1007/978-1-4939-1538-5_23
Vencken, Sebastian ; Tidi Maharani, Hassan ; McElvaney, Noel G. ; Smith, Stephen G J ; Greene, Catherine M. / miR-CATCH : MicroRNA capture affinity technology. RNA Interference: Challenges and Therapeutic Opportunities. Springer New York, 2014. pp. 365-373
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